Paroksetin HCl'nin UV-VIS ile Tayin Edilmesi için Farklı Bir Analitik Yöntem

Yıl 2021, Cilt: 3 Sayı: 2, 120 - 132, 11.10.2021

Figen Erek

Öz

Paroxetine HCl (PRX) 'in tabletten belirlenmesi için duyarlı, kolay ve seçici bir spektrofotometrik yöntem geliştirilmiştir. Bu yöntem, ilaç etken maddesinin yapısındaki ikincil amin grubu üzerinden renkli bir Cu-ditiokarbamat kompleksi oluşturulmasına dayanmaktadır. Paroxetin HCl ile oluşturulan kompleks, UV-VIS spektrofotometresi ile metilizobutilketon çözücüsü içinde 435 nm'de maksimum absorbans vermektedir. Geliştirilen yöntemde, çözücü cinsi (ekstraksiyon çözücüsü), NH3 konsantrasyonu, CS2 konsantrasyonu, Cu2 + konsantyasyonu ve reaksiyon süresinin etkisi araştırılmıştır. Daha sonra yöntem istatistiksel olarak doğrulanmıştır. Yöntemin validasyonu için ilk olarak Lambert-Beer yasasına uyan çalışma aralığı belirlenmiştir. Bu aralığın 0.005-0.16 mmol.L-1 olduğu bulunmuştur. Bu çalışma aralığında 0.9987 korelasyon katsayısı ile elde edilen kalibrasyon eğrisinin denklemi y = 6.9023x-0.0147'dir. Bu yöntemde tespit limiti ve kantitatif limit sırasıyla 0.0013 mmol.L-1 ve 0.0045 mmol.L-1 olarak bulunmuştur. Önerilen yöntemin PRX'in tablet formülasyonuna uygulanabileceği tespit edilmiştir.

Anahtar Kelimeler

Paroksetin HCl, Spektrofotometrik tayin, Bakır, Ditiyokarbamat

Destekleyen Kurum

Dicle Üniversitesi Bilimsel Araştırma Koordinatörlüğü

Proje Numarası

ECZACILIK:19.001

A Different Analytical Method for Determination of Paroxetine HCl by UV-VIS

Öz

For the determination of Paroxetine HCl (PRX) from tablet form a sensitive, easy and selective spectrophotometric method was developed. This method is based on the formation of a colored Cu-dithiocarbamate derivative complex on the basis of the secondary amine group in the structure of the drug substance. The colored complex gives maximum absorbance at 435 nm in methylisobuthylketone by UV-VIS spectrometer. In the method, the effect of solvent, NH3 concentration, CS2 concentration, Cu2+ concentration and reaction time were investigated. Then, the method was statistically validated. For the validation of the method, the working range that obeyed to the Lambert-Beer law was determined, firstly. The range was found to be 0.005-0.16 mmolL-1. The equation of the calibration curve obtained with 0.9987 correlation coefficient in the specified working range is y = 6.9023x-0.0147. In the method, limit of detection and limit of quantitative were found to be 0.0013 mmolL-1, and 0.0045 mmolL-1, respectively. It has been seen that the proposed method is applicable to tablet formulation of PRX.

Anahtar Kelimeler

Paroxetine HCl, copper, Spectrophotometric determination, dithiocarbamate

Proje Numarası

ECZACILIK:19.001

Kaynakça

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