HISTOPATHOLOGICAL CHANGES IN THE STOMACH MUCOSA OF RATS FED WITH GREAT SCALLOP (PECTEN MAXIMUS)

Yıl 2018, Cilt: 2 Sayı: 3, 105 - 114, 31.07.2018

Aslı Muratlı Mehmet Rıza Gezen

Öz

Heavy metals, industrial and household wastes and
pesticides are threats for the aquatic ecosystem. Polluted water sources are
streaming into the seas and cause pollution in these systems. Dardanelles is
exposed to pollution from the Marmara and Black Sea. Our previous studies
demonstrated that the water and mollusc from certain regions of the Dardanelles
contained heavy metal salts. The purpose of the study is to demonstrate the
histopathologic changes in the gastric tissues of rats which are fed with great
scallop (Pecten maximus) that are collected from the Çardak region of the
Dardanelles. Four groups of rats are included in the study, group 1 (n=6),
control group fed with standard rat food, group 2 (n=6), 75%  great scallop and 25% standard rat food
daily, group 3 (n=6), 75% great scallop and 25% standard rat food every two
days, group 4 (n=6), 75%  great scallop
and 25% standard rat food every three days. After the routine histopathologic
processing all gastric tissue samples are evaluated in terms of 8-hydroxy-2'
-deoxyguanosine (8-OHdG) immunoreactivity with light microscopy and image
analysis software. No histopathologic differences found in standard hematoxylin-eosin
(H.E.) stained gastric tissue samples of the control group. Second group showed
active chronic gastritis, third group showed less inflamation and chronic
gastritis compared with the second group and fourth group showed less
mononuclear inflammation compared to the second and third groups. In
immunohistochemical staining, 8-OHdG immunoreactivity in gastric epithelial
cells. 8-OHdG immunoreactivity was negative in stomach tissues in all
groups.  There was no statistically
significant difference between the groups that were fed every day, every other
day and every three days with great scallop (p>0.05). The results of our
studies showed that rats fed more with great scallops could produce gastritis
in the stomach.

Anahtar Kelimeler

Dardanelles, 8-hydroxy-2' -deoxyguanosine, immunohistochemistry, stomach, great scallop

Kaynakça

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