GENTAMİSİN YÜKLÜ POLİ(VİNİL ALKOL)/JELATİN NANOFİBERLERİN ELEKTROEĞİRME YÖNTEMİYLE YARA ÖRTÜSÜ MALZEMESİ OLARAK ÜRETİLMESİ

Abstract

İyi antimikrobiyal performansa ve cilt yenileme kabiliyetine sahip nanofiber polimer yapı iskelelerine dayalı yara örtüleri, yara enfeksiyonunu önlemek ve yara iyileşmesini hızlandırmak için umut verici seçeneklerdir. Bu çalışmada, yara örtüsü uygulamaları için Gentamisin (GEN) yüklü polivinil alkol ve jelatin (PVA/JEL) nanofiberleri elektroeğirme yöntemi ile başarıyla üretilmiştir. Üretilen nanofiberlerin mekanik mukavemetini arttırmak ve hızlı bozulmalarını önlemek için glutaraldehit (GA) buharı ile çapraz bağlama yapılmıştır. Nanofiberlerin taramalı elektron mikroskobu (SEM) görüntüleri incelendiğinde, boncuksuz düzgün bir yapıya sahip oldukları ve yüzeyde ilaç kristalleri ve kümeleri görülmediği gözlemlendi. Ayrıca PVA/JEL, PVA/JEL/0.25GEN ve PVA/JEL/0.5GEN nanofiberlerinin ortalama çapları sırasıyla 278±40 nm, 373± 68 nm ve 493± 105 nm olarak ölçüldü. Nanofiber içerisindeki ilaç miktarındaki artış fiber çaplarında az bir artışa neden olsa da fiber morfolojilerini olumsuz etkilememiştir. Fourier-dönüşümlü kızılötesi spektroskopisi (FTIR) ile PVA, JEL ve GEN arasındaki etkileşim kanıtlanmıştır. Çekme testine göre, PVA/JEL nanofiberi, 6.31 ± 0.23 MPa' lık bir gerilme mukavemetine sahiptir. Ağırlıkça %0.25 ve %0.5 GEN, bu PVA/JEL nanofiberlerine ayrı ayrı yüklendiğinde, gerilme mukavemetleri sırasıyla 4.30 ± 0.58 ve 3.95 ± 0.24 MPa idi. GEN miktarı arttıkça mekanik mukavemetinin saf nanofibere göre zayıfladığı gözlemlenmiştir. Antimikrobiyal aktivite sonuçlarına bakıldığında, PVA/JEL/0.25GEN nanofiberinde herhangi bir antibakteriyel etki görülmezken PVA/JEL/0.5GEN nanofiberinde antibakteriyel etki görülmüştür. Antibakteriyel etki görülen PVA/JEL/0.5GEN nanofiberinde ilaç salım çalışmaları gerçekleştirilmiştir. İlaç salım sonuçlarına göre, GEN' in nanofiberden 264 saate kadar uzayan bir salım profili sergilediği gözlemlenmiştir. Tüm sonuçlar değerlendirildiğinde, GEN içeren nanofiberler, doku mühendisliği ve yara örtüsü uygulamalarında umut verici bir potansiyele sahip olabilir.

Keywords

• Yara örtüsü
• Nanofiber
• Polimerler
• Elektroeğirme
• Gentamisin

Production of Gentamycin-Loaded Poly(Vinyl Alcohol)/Gelatin Nanofiber by Electrospinning Method as Wound Dressing Material

Abstract

Wound dressings based on nanofiber polymer scaffolds with good antimicrobial capacity and skin regeneration ability are hopeful alternatives for hindering wound infection and speeding wound healing. In this study, Gentamicin (GEN) loaded polyvinyl alcohol and gelatin (PVA/GEL) nanofibers were successfully produced by electrospinning for wound dressing applications. In order to increase the mechanical strength of the produced nanofibers and to prevent their rapid deterioration, crosslinking was done with glutaraldehyde (GA) vapor. When the scanning electron microscopy (SEM) images of the nanofibers were examined, it was observed that they had a smooth structure without beads and no drug crystals and clusters were observed on the surface. In addition, the mean diameters of PVA/JEL, PVA/JEL/0.25GEN and PVA/JEL/0.5GEN nanofibers were measured as 278±40 nm, 373±68 nm and 493±105 nm, respectively. Although the increase in the amount of drug in the nanofiber caused a slight increase in the fiber diameters, it did not adversely affect the fiber morphologies. The interaction between Fourier-transform infrared spectroscopy (FTIR) and PVA, GEL, and GEN has been proven. According to the tensile test, the PVA/JEL nanofiber has a tensile strength of 6.31 ± 0.23 MPa. When 0.25% and 0.5% by weight GEN were loaded into these PVA/JEL nanofibers separately, their tensile strengths were 4.30 ± 0.58 and 3.95 ± 0.24 MPa, respectively. It was observed that as the amount of GEN increased, its mechanical strength weakened compared to the pure nanofiber. Considering the antimicrobial activity results, no antibacterial effect was observed in the PVA/GEL/0.25GEN nanofiber, while the antibacterial effect was observed in the PVA/GEL/0.5GEN nanofiber. Drug release studies have been carried out on PVA/GEL/0.5GEN nanofibers with antibacterial effects. Based on the drug release results, it was observed that GEN exhibited a release profile extending from nanofiber up to 264 hours per hour. Considering all the results, GEN-containing nanofibers may have promising potential in tissue engineering and wound dressing applications.

Keywords

• Wound dressing
• Polymers
• Electrospinning
• Gentamicin
• Yara örtüsü
• Nanofiber
• Polimerler
• Elektroeğirme
• Gentamisin

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