Toxicity of Some Entomopathogenic Fungi Combined with Boric Acid Againts Adults of American Cockroach Periplaneta americana (L.) (Blattodea: Blattidae)

Yıl 2023, Cilt: 26 Sayı: 4, 795 - 805, 31.08.2023

İbrahim Ersin Bozdoğan Hasan Tunaz

https://doi.org/10.18016/ksutarimdoga.vi.1221835

Öz

In this study, the pathogenicity of some entomopathogenic fungi on American cockroach Periplaneta americana (L.) adults and the effect of the most effective fungus isolate combined with boric acid on the virulence of the fungus were investigated in laboratory conditions. According to the results obtained from the experiments, the most effective isolate was found as Beauveria bassiana 5-4 and when it was used on insects, it caused 0%, 66.6% and 93.3% mortality at the dose of 0.1×109 conidia cm-2 at 72, 96 and 120 hours, respectively. This fungus also caused 0%, 20% and 20% mortality, respectively, at dose of 0.05×109 conidia cm-2 in the same time period. When boric acid was used on insects, it caused 6.6%, 40% and 66.6% mortality at dose of 1 mg cm-2 at 72, 96 and 120 hours, respectively. When 0.1×109 conidia cm-2 dose of B. bassiana 5-4 was used together with 1 mg cm-2 dose of boric acid, 33.3%, 60% and 93.3% mortality occurred at 72, 96 and 120 hours, respectively. When 0.05×109 conidia cm-2 dose of B. bassiana 5-4 was used together with 1 mg cm-2 dose of boric acid, 33.3%, 60% and 86.6% mortality occurred at 72, 96 and 120 hours, respectively. The results showed that the combined use of boric acid and B. bassiana 5-4 increased the virulence of the fungus at short exposure times (72 and 96 hours), but did not significantly affect the virulence of the fungus at long exposure times (120 hours and above).

Anahtar Kelimeler

Periplaneta americana, Beauveria bassiana, Boric acid, Entomopathogen fungus, Biological control

Teşekkür

This work is part of the first author's master's thesis. Thanks to Prof. Dr. Mehmet Kubilay ER and Dr. Cebrail BARIŞ for providing entomopathogenic fungal cultures.

Bazı Entomopatojen Fungusların Borik Asitle Birlikte Kullanımının Amerikan Hamamböceği Periplaneta americana (L.) (Blattodea: Blattidae) Erginlerine Karşı Toksisitesi

Öz

Bu çalışmada, laboratuvar koşullarında bazı entomopatojen fungusların Amerikan hamamböceği Periplaneta americana (L.) erginlerine patojenitesi ve en etkili fungus izolatının borik asit ile birlikte kullanımının fungusun virülensliği üzerindeki etkisi araştırılmıştır. Testlerden elde edilen sonuçlara göre en etkili izolat Beauveria bassiana 5-4 olmuştur ve böcekler üzerinde tek başına kullanıldığında 72, 96 ve 120 saatlerde 0.1×109 konidi cm-2 dozunda sırasıyla %0, %66.6 ve %93.3 ölüm meydana getirmiştir. Bu fungus aynı zaman aralığında 0.05×109 konidi cm-2 dozunda sırasıyla %0, %20 ve %20 ölüm meydana getirmiştir. Borik asit böcekler üzerinde tek başına kullanıldığında 72, 96 ve 120 saatlerde 1 mg cm-2 dozunda sırasıyla %6.6, %40 ve %66.6 ölüm meydana getirmiştir. B. bassiana 5-4’ün 0.1×109 konidi cm-2 dozu borik asidin 1 mg cm-2 dozu ile birlikte kullanıldığında 72, 96 ve 120 saatlerde sırasıyla %33.3, %60 ve %93.3 ölüm meydana getirmiştir. B. bassiana 5-4’ün 0.05×109 konidi cm-2 dozu borik asidin 1 mg cm-2 dozu ile birlikte kullanıldığında sırasıyla 72, 96 ve 120 saatlerde %33.3, %60 ve %86.6 ölüm meydana getirmiştir. İstatistiksel analizler borik asit ve B. bassiana 5-4’ün kombine kullanımının kısa maruz kalma sürelerinde (72 ve 96 saat) fungusun virülanslığını artırdığını ancak uzun maruz kalma sürelerinde (120 saat ve üzeri) fungusun virülanslığına önemli bir etki etmediğini göstermiştir.

Anahtar Kelimeler

Periplaneta americana, Beauveria bassiana, Borik asit, Entomopatojen fungus, Biyolojik kontrol

Kaynakça

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