Geleneksel Türk Yoğurtlarından İzole Edilen Lactobacillus delbrueckii subsp. bulgaricus ve Streptococcus thermophilus Bakterilerinde İntra-/Ekstrasellüler Folat Üretimi

Yıl 2024, Cilt: 27 Sayı: 3, 704 - 717

Meryem Nur Zeydanlı Zehranur Yuksekdag Berat Çınar Acar

https://doi.org/10.18016/ksutarimdoga.vi.1295846

Öz

Bu çalışmada, zengin folat içerikli yoğurt yapımında starter kültür olarak kullanılabilecek bakterilerin araştırılması hedeflenmiştir. Bu amaçla Türkiye’nin çeşitli ilçelerindeki geleneksel yöntemlerle yapılan yoğurtlardan 31 Lactobacillus spp. ve 32 Streptococcus spp. olmak üzere toplam 63 bakteri izole edilmiştir. Elde edilen bakterilerin API 50 CH kiti ile biyokimyasal tanımlaması gerçekleştirilmiştir. Tesadüfen seçilen 4 Laktobasil ve 4 Streptokok izolatının 16S rDNA gen bölgesi ile moleküler tanımlaması gerçekleştirilmiştir. Folat içermeyen kültür ortamı (FACM), skimmilk ve MRS/Elliker besi ortamlarında üç farklı tampon uygulamasında (potasyum fosfat, sodyum fosfat ve sodyum asetat) bakterilerin hücre içi ve hücre dışı folat üretim miktarları tespit edilmiştir. Kullanılan besiyerleri ve tampon çözeltiler arasında anlamlı bir farklılık olup olmadığı istatistiksel olarak incelenmiştir. Skimmilk besiyerinde potasyum fosfat tamponu uygulamasında, Lactobacillus delbrueckii subsp. bulgaricus ZN1181 suşu 105,6 µg/L değeri ile en yüksek intrasellüler folat üretimine sahip olduğu tespit edilirken, Streptococcus thermophilus Z651 suşu FACM besiyeri ve potasyum fosfat tamponunda en yüksek ekstrasellüler folat üretimi (79,9 µg/L) göstermiştir.

Anahtar Kelimeler

Intrasellüler Folat, Ekstrasellüler Folat, Lactobacillus delbrueckii subsp. bulgaricus, Streptococcus thermophilus

Destekleyen Kurum

Gazi Üniversitesi

Proje Numarası

05/2013-07

Teşekkür

Bu çalışma, Gazi Üniversitesi Fen Bilimleri Enstitüsü Biyoloji Anabilim Dalında, 2014 yılında tamamlanan “Geleneksel Yöntemlerle Yapılan Yoğurtlardan İzole Edilen Lactobacillus delbrueckii subsp. bulgaricus ve Streptococcus thermophilus Türlerinde Folat Üretimi” isimli yüksek lisans tezinden türetilmiştir. Ayrıca, çalışmamız Gazi Üniversitesi Bilimsel Araştırma Projeleri (BAP) Birimi tarafından 05/2013-07 kodlu proje ile desteklenmiştir.

Production of Intra-/Extracellular Folate in Lactobacillus delbrueckii subsp. bulgaricus and Streptococcus thermophilus Bacteria Isolated from Traditional Turkish Yoghurts

Öz

This study, it was aimed to examined the bacteria that can be used as starter cultures in the generation of yoghurt with rich folate content. For this purpose, 31 Lactobacillus spp. and 32 Streptococcus spp. a total of 63 bacteria was isolated from yogurt made with traditional methods in various districts of Turkey. Biochemical identification of obtained bacteria was made with API 50 CH kit. Molecular identification of randomly selected 4 Lactobacillus and 4 Streptococus isolates with 16S rDNA gene regions were performed. Intracellular and extracellular folate production amounts of bacteria were designated in three different buffer applications (potassium phosphate, sodium phosphate and sodium acetate) in folate-free culture medium (FACM), skim milk and MRS/Elliker broth. It was statistically examined whether there was a significant difference between the media and buffer solutions used. In the application of potassium phosphate buffer in Skimmilk medium, Lactobacillus delbrueckii subsp. bulgaricus ZN1181 strain was found to have the highest intracellular folate production with a value of 105.6 µg/L, while Streptococcus thermophilus Z651 strain showed the highest extracellular folate production (79.9 µg/L) in FACM medium and potassium phosphate buffer.

Anahtar Kelimeler

Intracellular Folate, Extracellular Folate, Lactobacillus delbrueckii subsp. bulgaricus, Streptococcus thermophilus

Proje Numarası

05/2013-07

Kaynakça

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