Arjirofilik Nükleolar Organize Edici Bölgeler Non ST Elevasyonlu Miyokard İnfarktüsünde Hipoksik Yanıtın Yeni bir Belirteci Olabilir mi?

Abstract

Amaç: Non-ST elevasyonlu miyokard enfarktüsü (NSTEMI), bir akut koroner sendrom türüdür ve görülme sıklığı ST elevasyonlu miyokard enfarktüsüne benzer şekilde yüksektir. Nükleolar organize edici bölgeler (NORs), akrosentrik kromozomun ikincil yapısında yer alır ve bazıları arjirofilik olan proteinlerden ve ribozomal DNA'dan (rDNA) oluşur. NSTEMI hastalarında, hipokside artan AgNOR proteinlerinin değişimini araştırmayı amaçladık. Gereç ve Yöntem: Toplam 125 katılımcı, 63 NSTEMI hastası ve herhangi bir akut koroner sendrom tanısı olmayan 62 gönüllü çalışmaya dahil edildi. Bütün hastalara ekokardiyografi yapıldı ve her birey için hem ortalama AgNOR sayısı hem de toplam AgNOR alanı/toplam nükleer alan (TAA/TNA) tespiti yapıldı. Bulgular: Ortalama AgNOR sayısı ve TAA/TNA oranı NSTEMI grubunda kontrole göre anlamlı derecede yüksekti (p<0.001). TAA/TNA ile, kreatinin, hemoglobin, WBC(μl/ml), monosit, nötrofil, nötrofil/lenfosit oranı, monosit/lenfosit oranı arasında istatistiksel olarak anlamlı ilişkiler tespit edildi (tümü için p<0.05). Ayrıca ortalama AgNOR sayısı ile, açlık kan şekeri, HDL, WBC(μl/ml), monosit, nötrofil ve EF arasında istatistiksel olarak anlamlı ilişkiler tespit edildi (p<0.001). Sonuç: Her iki AgNOR protein miktarı, NSTEMI için bir markır olarak kullanılabilir. NSTEMI'de bazı prognostik bilgiler sağlayarak sonuçların tahmin edilmesine de katkıda bulunabilir.

Keywords

• AGNOR
• Hipoksi
• NOR
• NSTEMI

May Argyrophilic Nucleolar Organizer Regions be the New Marker of a Hypoxic Response in Non ST Elevation Myocardial İnfarction?

Abstract

Objective: Non-ST elevation myocardial infarction (NSTEMI) is a type of acute coronary syndrome and its’ incidence is similarly high to ST-elevation myocardial infarction. Nucleolar organizing regions (NORs) are located of the secondary structure of acrocentric chromosome and composed of proteins and ribosomal DNA (rDNA) some of which are argyrophilic. We aimed to investigate the change of AgNOR proteins, which increase in hypoxia, in patients with NSTEMI. Methods: A total of 125 participants, 63 patients with NSTEMI and 62 volunteers without any acute coronary syndrome were included in the study. Echocardiography was performed and both mean AgNOR Number and total AgNOR area/total nuclear area (TAA/TNA) were detected for each individuals. Results: The mean AgNOR number and TAA/TNA ratio were significantly higher in the NSTEMI group than control (p<0.001). Also, statistically significant relations between TAA/TNA and all of creatinine, hemoglobin, WBC(μl/ml), monocyte, neutrophil, neutrophil / lymphocyte ratio, monocyte / lymphocyte ratio were detected (p<0.05 for all). Also, statistically significant relations between mean AgNOR number and all of fasting blood sugar, HDL, WBC(μl/ml), monocyte, neutrophil, EF were detected (p<0.001). Conclusions: Both AgNOR protein amounts may be used as a marker for NSTEMI. It may also contribute to the prediction of the outcomes by providing some prognostic information in NSTEMI.

Keywords

• AgNOR
• Hypoxia
• NOR
• NSTEMI.

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