Interleukin-23R Gene Polymophisms in Patients with Diabetic Peripheral Polyneuropathy

Year 2022, , 406 - 410, 29.06.2022

Özgül Ocak , Fatma Sılan

https://doi.org/10.18521/ktd.1097853

Abstract

Objective: Proinflammatory and neurovascular changes are blamed in the pathogenesis of diabetic neuropathy. Although it is accepted that diabetes is a trigger for vascular inflammation, it has been suggested that inflammation itself may trigger diabetes. Interleukin-23 (IL-23) is a pro-inflammatory cytokine secreted by activated macrophages and dendritic cells. Interleukin-23R is known to have a critical role in chronic inflammatory diseases. The aim of this study is to determine the relationship between IL-23R polymorphism and diabetic peripheral neuropathy.
Method: 50 diabetic peripheral neuropathy patients who applied to Neurology outpatient clinic, and 52 healthy controls compatible with the patient group in terms of age and gender were included. Electromyography was performed on all of the volunteers, who agreed to participate in the study, and 2 ml of blood samples were taken into tubes with EDTA, and the IL-23R gene polymorphism was analyzed using the pyrosequencing method.
Results: IL-23R gene variants rs2201841, rs199542433, rs201052419, rs11209026 were analyzed in diabetic peripheral neuropathy (DPN) patients and control group. While we investigate IL23R polymorphisms we didn’t find any significant differences between patient and control groups. But when we use odds ratios, rs2201841 seems to have a protective role, and rs199542433 in both dominant and recessive models and rs11209026 only recessive model seem to be related 10 fold higher risks for DPN.
Conclusion: IL-23R gene polymorphism has been shown to be associated with many autoimmune and inflammatory diseases. It is known that inflammation has an important effect on diabetes. The frequency of IL-23R gene polymorphism was not significant in diabetic peripheral neuropathy. Our study is the only and first study investigating the role of IL-23R gene polymorphism in diabetic peripheral neuropathy. Ethnicity is very important in genetic studies, and it will give us more clear information for the future to carry out this study in patients with other ethnic origins and to recruit larger study groups.

Keywords

Diabetic peripheral neuropathy, inflammation, genetics, interleukin-23R (IL-23R) gene polymorphism

Supporting Institution

Çanakkale Onsekiz Mart University Scientific Research Projects Coordination Unit

Project Number

TSA-2020-3471

Diyabetik Periferik Polinöropatili Hastalarda İnterlökin-23R Gen Polimofizmleri

Abstract

Amaç: Diyabetik nöropatinin patogenezinde proinflamatuar ve nörovasküler değişiklikler suçlanmaktadır. Diyabetin vasküler inflamasyonu tetiklediği kabul edilse de inflamasyonun da diyabeti tetikleyebileceği öne sürülmüştür. İnterlökin-23 (IL-23) aktive makrofajlar ve dendritik hücreler tarafından salgılanan proinflamatuar bir sitokindir. Interleukin-23R'nin kronik inflamatuar hastalıklarda kritik bir rolü olduğu bilinmektedir. Bu çalışmanın amacı, IL-23R polimorfizmi ile diyabetik periferik nöropati arasındaki ilişkiyi incelemektir.
Yöntem: Nöroloji polikliniğine başvuran 50 diyabetik periferik nöropati hastası ve hasta grubuna yaş ve cinsiyet açısından uyumlu 52 sağlıklı kontrol çalışmaya dahil edildi. Çalışmaya katılmayı kabul eden gönüllülerin tamamına elektromiyografi uygulandı ve EDTA'lı tüplere 2 ml kan örneği alındı. Pyrosequencing yöntemi ile IL-23R gen polimorfizmi analiz edildi.
Bulgular: IL-23R gen varyantları rs2201841, rs199542433, rs201052419, rs11209026 diyabetik periferik nöropati (DPN) hastalarında ve kontrol grubunda analiz edildi. IL23R polimorfizmleri sıklıkları açısından hasta ve kontrol grupları arasında anlamlı bir fark saptanmadı. Ancak, odd’s oranlarına bakıldığında, rs2201841'in koruyucu rolü var gibi görünmekte, rs199542433 hem baskın hem de çekinik modellerde ve rs11209026 sadece çekinik modelde, DPN için 10 kata kadar daha yüksek risklerle ilişkili olabileceği görünmektedir.
Sonuç: IL-23R gen polimorfizminin birçok otoimmün ve inflamatuar hastalık ile ilişkili olduğu gösterilmiştir. İnflamasyonun diyabet üzerinde önemli bir etkisi olduğu bilinmektedir. Diyabetik periferik nöropatide IL-23R gen polimorfizminin sıklığı anlamlı değildi. Çalışmamız diyabetik periferik nöropatide IL-23R gen polimorfizminin rolünü araştıran tek ve ilk çalışmadır. Etnik köken, genetik çalışmalarda çok önemlidir ve bu çalışmanın başka etnik kökene sahip hastalarda yapılması ve daha geniş çalışma gruplarının alınması, bize ilerisi için daha net bilgiler verecektir.

Keywords

Diyabetik periferik nöropati, inflamasyon, genetik, interlökin-23R (IL-23R) gen polimorfizmi

Project Number

TSA-2020-3471

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