D Vitamini Yetersizliğinin Biyobelirteçleri: Sağlıklı Türk bireylerde D vitamini Metabolit Düzeyleri 25-Hidroksivitamin D2 düzeylerine bağlı değildir

Abstract

Amaç: Hastaya özgü faktörler yeterlilik için gerekli ek D vitamini dozunu etkileyebilir ve D vitamini takviyesi reçete etmek için hangi metabolitin klinik olarak anlamlı kabul edildiğini doğrulamak önemlidir. Bu çalışmada, sağlıklı bir Türk popülasyonunda 25(OH)D2 ve serbest D vitamini düzeyleri ile D vitamini eksikliği arasındaki ilişki değerlendirilmiştir. Yöntem: Her iki cinsiyeti temsil eden 18 yaş üstü 92 sağlıklı yetişkinden kan örnekleri alınmıştır (n = 38 erkek ve n = 54 kadın). Toplam 25(OH)D konsantrasyonları CMIA adı verilen bir immünoassay yöntemi kullanılarak belirlenmiştir. Ayrıca, serumdaki 25(OH)D3 ve D2 vitamini seviyeleri sıvı kromatografi-kütle spektrometresi (LC-MS) kullanılarak ölçülmüştür. Serbest 25(OH)D hesaplaması Bikle yöntemine göre yapılmıştır. Bulgular: Total 25(OH)D3 düzeyi ölçülen katılımcıların %54'ünde 20 ng/mL'nin altındaydı. Toplam 25(OH)D3 konsantrasyonu 20 ng/mL veya daha yüksek olanların ortalama serum 25(OH)D3 ve serbest D vitamini ölçüm seviyeleri 20 ng/mL'nin altında olanlara kıyasla anlamlı derecede yüksekti (P < 0.001). Buna karşılık, ortalama 25(OH)D2 konsantrasyonu D vitamini eksikliği kriterlerine göre önemli bir değişiklik göstermemiştir. Ayrıca, 25(OH)D2 serum seviyeleri, serbest D vitamini konsantrasyonlarından bağımsız olarak bireyler arasında tutarlıydı. Sonuç: LC-MS ile ölçülen ortalama serum 25(OH)D3 ve serbest D vitamini konsantrasyonları, otomatik bir sistemle ölçülen toplam 25(OH)D3 konsantrasyonunu etkiler ve D vitamini eksikliğini gösterir. Bununla birlikte, serumdaki ortalama 25(OH)D2 konsantrasyonu, D vitamini eksikliği kriterlerine ve serbest D vitamini konsantrasyonuna göre bireyler arasında farklılık göstermemiştir, bu nedenle doğrudan D vitamini eksikliğini göstermez.

Keywords

• D vitamini
• 25-Hidroksivitamin D2
• 25-Hidroksivitamin D3
• serbest D vitamini

Project Number

Herhangi bir projeden destek alınmamıştır.

Biomarkers of Vitamin D Sufficiency: Vitamin D metabolite levels do not depend on 25-Hydroxyvitamin D2 levels in healthy Turkish individuals

Abstract

Objective: Patient-specific factors may influence the dose of supplemental vitamin D necessary for adequacy, and it is important to verify which metabolite is considered clinically meaningful in order to prescribe vitamin D supplements. We assessed the relationship between 25(OH)D2 and free vitamin D levels in healthy Turkish population and those with vitamin D deficiency. Methods: Blood samples were taken from 92 healthy adults over the age of 18, representing both genders (n = 38 males and n = 54 females). Total 25(OH)D concentrations were determined using an immunoassay method called CMIA. In addition, 25(OH)D3 and vitamin D2 levels in serum were measured using liquid chromatography-mass spectrometry (LC-MS). Free 25(OH)D was measured and calculated according to the Bikle method. Results: Among the participants, 54% exhibited total 25(OH)D3 levels below 20 ng/mL. Those with total 25(OH)D3 concentrations of 20 ng/mL or higher had significantly elevated mean serum levels of 25(OH)D3 and free vitamin D compared to those with levels below 20 ng/mL (P < 0.001). In opposition, the mean concentration of 25(OH)D2 did not show significant variation based on the criteria for vitamin D sufficiency. Additionally, serum levels of 25(OH)D2 were consistent across individuals, regardless of their free vitamin D concentrations. Conclusion: The mean concentrations of serum 25(OH)D3 measured by LC-MS and free vitamin D affects the total 25(OH)D3 concentration as calculated by an automated system and indicates vitamin D deficiency. The mean concentration of 25(OH)D2 in serum, however, didn't differ among individuals according to vitamin D deficiency criteria and concentration of free vitamin D, thus does not directly indicate vitamin D deficiency.

Keywords

• D vitamini
• 25-Hidroksivitamin D2
• 25-Hidroksivitamin D3
• serbest D vitamini

Project Number

Herhangi bir projeden destek alınmamıştır.

Ethical Statement

Biruni Üniversitesi Girişimsel Olmayan Etik Kurul tarafından onay alınmıştır. Etik Kurul onay formuda sisteme yüklenmiştir.

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