Levels of Serum M30 and M65 Proteins as Biomarkers of Apoptosis in Children Exposed to Passive Smoking

Abstract

Background: DNA damage associated with passive smoking has been shown in children. Severe DNA damage can change the apoptosis process of the cell.  M30 and M65 biomarkers are markers showing the apoptosis process and can be quantitatively measured. We aimed to determine M30 and M65 levels as apoptosis biomarkers in children exposed to passive smoking.

Methods: A total of 79 children were included in the study. Urine cotinine level was measured with the chemiluminescence method and the levels of apoptosis biomarkers M30 and M65 were measured in peripheral venous blood with PEVIVA/ALEXIS ELISA kits.

Results: The mean serum M30 level of the group exposed to smoking was found to be significantly low compared to that of the control group (p=0.01). The serum M65 value was not significant in either group (p=0.1). No correlation was determined between the mean cotinine level and M30 (r=0.25, p=0.02) or M65 (r=0.16, p=0.15).

Conclusion: The results of this study showed that the apoptosis biomarker M30 levels of the children exposed to passive smoking were low compared to those of the control group which can be interpreted as apoptosis inhibition in exposure to cigarette smoke. Further clinical studies are needed.

Keywords

• Apoptosis,M30,M65,cotinine,nicotine

Pasif Sigara İçen Çocuklarda Apotozis Biyobelirteci Olarak Serum M30 ve M65 Protein Düzeyleri

Abstract

Amaç: Pasif sigara içiciliğine bağlı DNA hasarı çocuklarda gösterilmiştir. Ciddi DNA hasarı, hücrenin apoptoz sürecini değiştirebilir. M30 ve M65 biyobelirteçleri apoptoz sürecini gösteren belirteçlerdir ve kantitatif olarak ölçülebilir. Pasif sigara içiciliğine maruz kalan çocuklarda M30 ve M65 düzeylerini apoptoz biyobelirteçleri olarak belirlemeyi amaçladık.

Yöntemler: Çalışmaya toplam 79 çocuk dahil edildi. İdrar kotinin düzeyi kemilüminesans yöntemiyle ölçüldü ve periferik venöz kanda apoptoz biyobelirteçleri olan M30 ve M65 düzeyleri PEVIVA / ALEXIS ELISA kitleri ile ölçüldü.

Bulgular: Sigaraya maruz kalan grubun ortalama M30 düzeyi kontrol grubuna göre anlamlı derecede düşük bulundu (p = 0.01). Serum M65 değeri her iki grupta da anlamlı değildi (p = 0.1). Ortalama kotinin düzeyi ile M30 (r = 0.25, p = 0.02) veya M65 (r = 0.16, p = 0.15) arasında korelasyon saptanmadı.

Sonuç: Bu çalışmanın sonuçları, pasif sigara içiciliğine maruz kalan çocukların apoptoz biyobelirteçi olan M30 düzeylerinin, kontrol grubuna göre kıyaslandığında sigara dumanına maruziyette apoptoz inhibisyonu olarak yorumlanabilecek şekilde düşük olduğunu gösterdi. İleri klinik çalışmalara ihtiyaç duyulmaktadır.

Keywords

• Apoptoz,M30,M65,cotinine,nicotine

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