Otizm Spektrum Bozukluğu ile Dikkat Eksikliği Hiperaktivite Bozukluğu Olan Çocuklarda Apoptotik Hücre Tabanlı Sistemik Nötrofil-Lenfosit Oranı

Abstract

Amaç: Son yıllarda, Nötrofil / Lenfosit oranının (NLR) sistemik inflamasyonun bir belirteci olduğu gösterilmiştir ve maligniteler ve kronik inflamatuar hastalıklar gibi birçok hastalıkta prognoz ile ilişkilidir. Önceki çalışmalara dayanarak, NLR ve hücresel morfolojik mekanizmanın inflamasyonla ilişkili hastalıklarda dikkat eksikliği hiperaktivite bozukluğu (DHB) ve otizm spektrum bozuklukları (ASD)’nın birlikte çalışıldığı bir bulgu yoktur. Araştırmamızda bu parametrelerin nörogelişimsel bozukluklar etiyopatogenezine etkisini ve ilişkisini değerlendirdik.

Gereç ve Yöntem: DHB ve ASD tanısı alan 30 sağlıklı ve 30 hasta, üçüncü basamak bir hastanede psikiyatri kliniğinde değerlendirildi. Tüm gruplarda hemogram profiline bakılarak NLR sayısı istatistiksel olarak karşılaştırıldı. Ayrıca hücrelerin apoptotik evresini belirlemek üzere 2 farklı yöntemle boyandı. DHB, ASD ve kontrol grubunun apoptotik mekanizması karşılaştırılabilir şekilde görüntülendi.

Bulgular: DHB ve ASD tanısı alan hastalarda NLR değerleri kontrole kıyasla anlamlı derecede yüksekti; lenfosit sayısı hasta gruplarında anlamlı olarak düşük bulundu. Apoptotik morfoloji, hastalık artışı derecesi ile korele olarak farklılık gösterdi.

Sonuç: Bu parametre, psikiyatrik hastalıklara yakalanma riski olan durumlarda, erken tanıda kolay uygulanabilir bir yöntem olarak kullanılabilir. Nötrofil/Lenfosit oranının apoptotik görüntüleme ile pozitif ilişkisi, nörodejeneratif patolojilerde hücresel bozulmanın bir belirtecini gösterir.

Keywords

• Hemogram profili
• ASD
• ADHD
• apoptotik tunel değişimi
• NLR

The systemic cell apoptotic-based neutrophil–lymphocyte ratio: Experience in children diagnosed with ADHD and Autism Spectrum Disorder

Abstract

Objective: In recent years, the rate of Neutrophil/Lymphocyte ratio (NLR) has been shown to be a marker of systemic inflammation and is associated with prognosis in many diseases like malignancies and chronic inflammatory diseases. Based on previous studies, there is not any finding that the NLR and cellular morphological mechanism studied together in inflammation-related diseases; attention deficit hyperactivity disorder(ADHD) and autism spectrum disorders(ASD). We assessed the effect and association of these parameters on the etiopathogenesis of neurodevelopmental disorders.

Material and Method: 30 healthy and 30 patients who were diagnosed with ADHD and ASD were evaluated in the psychiatry department at a tertiary hospital. In whole groups, the hemogram profile have been analyzed. NLR number was statistically compared. However, the apoptotic stage of cells were stained with 2 different methods. Apoptotic mechanisms of ADHD, ASD and control group were comparably displayed.

Results: NLR values in patients diagnosed with ADHD and ASD were significantly higher compare to control; lymphocyte count was found significantly lower level in patient groups. Apoptotic morphology becomes evident as the degree of disease increment.

Conclusion: This parameter can be used as an easily applicable method is estimated to be at risk for psychiatric diseases. The positive association of NLR with apoptotic imaging indicates a marker of cellular degradation with neurodegenerative disorders.

Keywords

• Hemogram profile
• ASD
• ADHD
• Apoptotic tunnel variation
• NLR

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