Evaluation of the Protective Effect of The Cup Therapy on the Epileptic Seizure in Rats

Year 2021, , 606 - 613, 18.10.2021

Ayhan Çetinkaya , Esra Fidan Selim Göksu Bihter Bozat Serif Demir

https://doi.org/10.18521/ktd.777484

Abstract

Objective: Cup therapy has an important place in traditional and complementary medicine applications. The purpose of our study, this is the first time to investigate the protective effect of cup therapy in rats on experimentally generated epileptic seizures in new rat modeling created by different anatomic regions.
Methods: In our study, 42 Wistar albino rats 3-4 months old (200-250 g) were used. Four other groups (n=7) except G3 and G4, 24 hours after cup therapy application, PTZ (35 mg/kg, sc., 0.2 cc) was injected, followed by behavioral epilepsy scoring.
Results: In the 'Open Area' and 'Elevated Plus Maze' tests, there was no behavioral difference between the cup therapy group and the control group (p > 0.05). Given all the parameters, the G4 group significantly reduces the seizure compared to other groups (p < 0.05). There is a significant difference in G2, G5 and G6 groups compared to G3 in the phases parameter (p < 0.05).
Conclusions: In this study, the new wet cup therapy (G2), which was applied for the first time, had a protective effect on seizures. G2, G5 and G6 groups are observed to suppress seizures compared to G3. Our findings are expected to contribute greatly to animal model analysis in the future.

Keywords

Epilepsy, Seizure, Cup Therapy, Hijama, Rat, Animal Model

Kupa Terapisinin Ratlarda İndüklenen Epileptik Nöbet Üzerine Koruyucu Etkisinin Değerlendirilmesi

Abstract

Amaç: Geleneksel ve tamamlayıcı tıp uygulamaları içerisinde kupa terapisi önemli bir yer almaktadır. Çalışmamızın amacı, ilk kez sıçanlarda kupa terapisinin farklı anatomik bölge tayini ile oluşturulan yeni rat modellemesinde, deneysel olarak oluşturulmuş epileptik nöbetler üzerine koruyucu etkisinin araştırılmasıdır.
Yöntem: Çalışmamızda, 42 adet Wistar albino cinsi 3-4 aylık (200-250 gr) sıçanlar kullanılmıştır. G3 ve G4 dışındaki diğer dört gruba (n=7), kupa terapisi uygulamasından 24 saat sonra, PTZ (35 mg/kg, sc., 0,2 cc) enjekte edildi ve ardından davranışsal epilepsi skorlaması yapılmıştır.
Bulgular: Toplamda 42 adet Wistar albino sıçan rastgele 6 gruba (n:7) ayrılmıştır. G1 grubuna "yeni kuru kupa", G2’ye ise "yeni yaş kupa" modeli uygulanmıştır. G3 grubunda, ''epilepsi modeli'' (PTZ, 35 mg/kg) oluşturulmuş ve G4’e diazepam (2,5 mg/kg) verilmiştir. G5 ve G6 gruplarına ise ''kuru kupa'' ve '' yaş kupa '' modelleri sırasıyla uygulanmıştır. Kupa terapilerinden 24 saat sonra, sıçanlara PTZ enjekte edilmiş ve daha sonra tüm gruplardaki sıçanların epilepsi davranış skorları 20-30 dakika boyunca kaydedilmiştir.
Sonuç: Bu çalışmada, ilk defa uygulanmış olan yeni yaş kupa terapisinin (G2) nöbetler üzerine koruyucu etkisi bulunmuştur. G2, G5 ve G6 gruplarının G3’e kıyasla nöbeti baskıladığı gözlemlenmektedir. Bulgularımızın ileride bu konuda yapılacak hayvan modeli analizlerine büyük ölçüde katkı sağlaması beklenmektedir.

Keywords

Epilepsi, Nöbet, Kupa terapisi, Hacamat, Rat, Hayvan modeli

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