Gül Yağının Sıçanlarda Penisilin İle Tetiklenen Epileptiform Aktivite Üzerine Etkileri: Elektrofizyolojik Bir Çalışma
Öz
Amaç: Gül yağı analjezik, antispazmodik, antioksidan ve nöroprotektif etkilere sahiptir. Anti-epileptik etki üzerine yapılan çalışmalar ise yetersizdir. Sunulan çalışmada, gül yağının sıçanlarda penisilin ile tetiklenen epilepsi modelinde epileptiform aktivite üzerine akut etkilerinin araştırılması amaçlanmıştır.
Gereç ve Yöntem: 230-260 gr ağırlığında kırk iki erkek wistar sıçan her grupta yedi olmak üzere altı gruba ayrıldı. Gruplar kontrol (penisilin), yalnız gül yağı, diazepam ve gül yağının 100, 500 ve 1000 mg/kg olmak üzere farklı dozlarından oluşmaktadır. Sıçanlara penisilin uygulamasından 30 dakika önce gül yağının farklı dozları uygulandı. Penisilin uygulamasından sonra 2 saat boyunca her bir hayvandan elektrokortikogram kayıtları alındı.
Bulgular: Gül yağının sadece 100 mg/kg dozu epileptiform aktivitenin epileptik diken-dalga frekansını önemli derecede azalttı. Bununla birlikte epileptiform aktivite latens ve diken-dalga amplitüdü açısından karşılaştırıldığında gruplar arasında anlamlı bir fark yoktu.
Sonuç: Gül yağının akut olarak uygulanması sıçanlarda, penisilin ile tetiklenen epileptiform aktivitenin diken-dalga frekansını azaltmaktadır. Böylece bulgularımız gül yağının anti-epileptik etkilere sahip olduğunu göstermektedir.
Anahtar Kelimeler
Kaynakça
- REFERENCES1. Thurman DJ, Beghi E, Begley CE et al. ILAE Commission on Epidemiology. Standards for epidemiologic studies and surveillance of epilepsy. Epilepsia. 2011; 52(7): 2–26. 2. Ngugi AK, Kariuki SM, Bottomley C, et al. Incidence of epilepsy: A systematic review and meta-analysis. Neurology. 2011; 77(10):1005-12. 3. Sucher NJ, Carles MC. A pharmacological basis of herbal medicines for epilepsy. Epilepsy Behav. 2015;52(Pt B):308-18.4. Zhu HL, Wan JB, Wang YT, et al. Medicinal compounds with antiepileptic/anticonvulsant activities. Epilepsia. 2014;55(1):3-16.5. White HS. Animal Models for Evaluating Antiepileptogenesis. In: Noebels JL, Avoli M, Rogawski MA, Olsen RW, Delgado-Escueta AV, editors. Jasper's Basic Mechanisms of the Epilepsies [Internet]. 4th edition. Bethesda (MD): National Center for Biotechnology Information (US); 2012. Available from http://www.ncbi.nlm.nih.gov/books/NBK98173/. (accessed January 23, 2018).6. Ankarali S, Beyazcicek E, Ankarali H, et al. The Effect of Rapamycin on Penicillin- Induced Epileptiform Activity in Rats: An Electrophysiological Study. Anatol Clin. 2016; 21(3): 197-206.7. Akdogan I, Adiguzel E, Yilmaz I, et al. Penicillin-induced epilepsy model in rats: Dose-dependant effect on hippocampal volume and neuron number. Brain Res. Bull. 2008;77:172–7. 8. Shi Y, Dong JW, Zhao JH, et al. Herbal Insomnia Medications that Target GABAergic Systems: A Review of the Psychopharmacological Evidence. Curr. Neuropharmacol. 2014; 12 : 289–302. 9. Campbell EL, Chebib M, Johnston GAR. The dietary flavonoids apigenin and (-)-pigallocatechin gallate enhance the positive modulation by diazepam of the activation by GABA of recombinant GABA A receptors. Biochem. Pharmacol. 2004; 68 ; 1631–38. 10. Hosseini M, Ghasemzadeh Rahbardar M, Sadeghnia HR, et al. Effects of different extracts of Rosa damascena on pentylenetetrazol-induced seizures in mice. Zhong Xi Yi Jie He Xue Bao. 2011; 9(10):1118-24. 11. Sayyah M, Beheshti S, Shokrgozar MA, et al. Antiepileptogenic and anticonvulsant activity of interleukin-1h in amygdala-kindled rats. Exp. Neurol. 2005; 191: 145–153. 12. Baydar H. Oil Bearing Rose ( Rosa damascena Mill .) Cultivation and Rose Oil Industry in Turkey. Euro Cosmetics. 2006; 14 (6):13-7.13. Abbasi Maleki N, Abbasi Maleki S, Bekhradi R. Suppressive effects of rosa damascena essential oil on naloxone- precipitated morphine withdrawal signs in male mice. Iran J Pharm Res. 2013;12(3):357-61.14. Boskabady MH, Kiani S, Rakhshandah H. Relaxant effects of Rosa damascena on guinea pig tracheal chains and its possible mechanism(s). J Ethnopharmacol. 2006; 106(3):377-82.15. Boskabady MH, Shafei MN, Saberi Z, et al. Pharmacological Effects of Rosa Damascena. Iranian Journal of Basic Medical Sciences. 2011;14(4):295-307.16. Basım E, Basım H. Note: Evaluation of antibacterial activity of essential oil of Rosa damascena on Erwinia amylovora. Phytoparasitica. 2004; 32:409–12. 17. Ozkan G, Sagdiç O, Baydar NG, et al. Note: Antioxidant and Antibacterial Activities of Rosa Damascena Flower Extracts. Food Sci Technol Int. 2004; 10: 277–81. 18. Rakhshandeh H, Vahdati-Mashhadian N, Dolati K, et al. Antinociceptive effect of Rosa damascena in mice. J Biol Sci. 2008; 8: 176–180.19. Fukada M, Kano E, Miyoshi M, et al. Effect of "rose essential oil" inhalation on stress-induced skin-barrier disruption in rats and humans. Chem Senses. 2012; 37(4):347-56. 20. Ashrafzadeh F, Rakhshandeh H, Mahmodi E. Rosa damascena oil: an adjunctive therapy for pediatric refractory seizures. Iran J Child Neurol. 2007; 1:13–7. 21. Ramezani R, Moghimi A, Rakhshandeh H, et al. The effect of Rosa damascena essential oil on the amygdala electrical kindling seizures in rat. Pak J Biol Sci. 2008; 11(5):746-51. 22. Kheirabadi M, Moghimi A, Rakhshande H, et al. Evaluation of the anticonvulsant activities of rosa damascena on the PTZ induced seizures in wistar rats. J Biol Sci. 2008; 8: 426-30. 23. Cakil D, Yildirim M, Ayyildiz M, et al. The effect of co-administration of the NMDA blocker with agonist and antagonist of CB1-receptor on penicillin-induced epileptiform activity in rats. Epilepsy Res. 2011; 93(2-3):128-37. 24. De Sousa DP, Gonçalves JCR, Quintans-Junior L. Study of anticonvulsant effect of citronellol, a monoterpene alcohol, in rodents. Neurosci Lett 2006; 401:231-5.25. Brito RG, Santos PL, Prado DS, et al. Citronellol reduces orofacial nociceptive behaviour in mice - evidence of involvement of retrosplenial cortex and periaqueductal grey areas. Basic Clin Pharmacol Toxicol. 2013;112(4):215-21.
The Effect of Rose Oil on Penicillin-Induced Epileptiform Activity in Rats: An Electrophysiological Study
Öz
Objective: Rose oil (from Rosa damascene) has several effects which are analgesic, antispasmodic, antioxidant and neuroprotective role. Its antiepileptic effect has not been yet studied enough. In the present study, it was aimed to investigate acute effects of rose oil on the epileptiform activity in penicillin-induced epilepsy model in rats.
Methods: Forty-two male Wistar rats weighing 230 to 260 g were divided into six groups with seven rats in each group. Control (+Penicillin), RO alone, Diazepam, and different doses of Rose oil including 100, 500 and 1000 mg/kg. Rats were pretreated with different doses of RO 30 min prior to penicillin treatment. Electrocorticogram recordings were taken from each animal for 2 hours after penicillin treatment.
Results: Only the dose of 100 mg/kg of rose oil reduced significantly epileptic spike-wave frequency of epileptiform activity. However, comparing in terms of latency and spike-wave amplitude of epileptiform activity, there were no significant difference between the groups.
Conclusions: In conclusion, acute administration of rose oil reduces spike-wave frequency of penicillin-induced epileptiform activity in rats. Therefore, these findings indicate that rose oil has antiepileptic effects.
Anahtar Kelimeler
Rose oil Epileptiform activity Epilepsy Electrocorticography
Kaynakça
- REFERENCES1. Thurman DJ, Beghi E, Begley CE et al. ILAE Commission on Epidemiology. Standards for epidemiologic studies and surveillance of epilepsy. Epilepsia. 2011; 52(7): 2–26. 2. Ngugi AK, Kariuki SM, Bottomley C, et al. Incidence of epilepsy: A systematic review and meta-analysis. Neurology. 2011; 77(10):1005-12. 3. Sucher NJ, Carles MC. A pharmacological basis of herbal medicines for epilepsy. Epilepsy Behav. 2015;52(Pt B):308-18.4. Zhu HL, Wan JB, Wang YT, et al. Medicinal compounds with antiepileptic/anticonvulsant activities. Epilepsia. 2014;55(1):3-16.5. White HS. Animal Models for Evaluating Antiepileptogenesis. In: Noebels JL, Avoli M, Rogawski MA, Olsen RW, Delgado-Escueta AV, editors. Jasper's Basic Mechanisms of the Epilepsies [Internet]. 4th edition. Bethesda (MD): National Center for Biotechnology Information (US); 2012. Available from http://www.ncbi.nlm.nih.gov/books/NBK98173/. (accessed January 23, 2018).6. Ankarali S, Beyazcicek E, Ankarali H, et al. The Effect of Rapamycin on Penicillin- Induced Epileptiform Activity in Rats: An Electrophysiological Study. Anatol Clin. 2016; 21(3): 197-206.7. Akdogan I, Adiguzel E, Yilmaz I, et al. Penicillin-induced epilepsy model in rats: Dose-dependant effect on hippocampal volume and neuron number. Brain Res. Bull. 2008;77:172–7. 8. Shi Y, Dong JW, Zhao JH, et al. Herbal Insomnia Medications that Target GABAergic Systems: A Review of the Psychopharmacological Evidence. Curr. Neuropharmacol. 2014; 12 : 289–302. 9. Campbell EL, Chebib M, Johnston GAR. The dietary flavonoids apigenin and (-)-pigallocatechin gallate enhance the positive modulation by diazepam of the activation by GABA of recombinant GABA A receptors. Biochem. Pharmacol. 2004; 68 ; 1631–38. 10. Hosseini M, Ghasemzadeh Rahbardar M, Sadeghnia HR, et al. Effects of different extracts of Rosa damascena on pentylenetetrazol-induced seizures in mice. Zhong Xi Yi Jie He Xue Bao. 2011; 9(10):1118-24. 11. Sayyah M, Beheshti S, Shokrgozar MA, et al. Antiepileptogenic and anticonvulsant activity of interleukin-1h in amygdala-kindled rats. Exp. Neurol. 2005; 191: 145–153. 12. Baydar H. Oil Bearing Rose ( Rosa damascena Mill .) Cultivation and Rose Oil Industry in Turkey. Euro Cosmetics. 2006; 14 (6):13-7.13. Abbasi Maleki N, Abbasi Maleki S, Bekhradi R. Suppressive effects of rosa damascena essential oil on naloxone- precipitated morphine withdrawal signs in male mice. Iran J Pharm Res. 2013;12(3):357-61.14. Boskabady MH, Kiani S, Rakhshandah H. Relaxant effects of Rosa damascena on guinea pig tracheal chains and its possible mechanism(s). J Ethnopharmacol. 2006; 106(3):377-82.15. Boskabady MH, Shafei MN, Saberi Z, et al. Pharmacological Effects of Rosa Damascena. Iranian Journal of Basic Medical Sciences. 2011;14(4):295-307.16. Basım E, Basım H. Note: Evaluation of antibacterial activity of essential oil of Rosa damascena on Erwinia amylovora. Phytoparasitica. 2004; 32:409–12. 17. Ozkan G, Sagdiç O, Baydar NG, et al. Note: Antioxidant and Antibacterial Activities of Rosa Damascena Flower Extracts. Food Sci Technol Int. 2004; 10: 277–81. 18. Rakhshandeh H, Vahdati-Mashhadian N, Dolati K, et al. Antinociceptive effect of Rosa damascena in mice. J Biol Sci. 2008; 8: 176–180.19. Fukada M, Kano E, Miyoshi M, et al. Effect of "rose essential oil" inhalation on stress-induced skin-barrier disruption in rats and humans. Chem Senses. 2012; 37(4):347-56. 20. Ashrafzadeh F, Rakhshandeh H, Mahmodi E. Rosa damascena oil: an adjunctive therapy for pediatric refractory seizures. Iran J Child Neurol. 2007; 1:13–7. 21. Ramezani R, Moghimi A, Rakhshandeh H, et al. The effect of Rosa damascena essential oil on the amygdala electrical kindling seizures in rat. Pak J Biol Sci. 2008; 11(5):746-51. 22. Kheirabadi M, Moghimi A, Rakhshande H, et al. Evaluation of the anticonvulsant activities of rosa damascena on the PTZ induced seizures in wistar rats. J Biol Sci. 2008; 8: 426-30. 23. Cakil D, Yildirim M, Ayyildiz M, et al. The effect of co-administration of the NMDA blocker with agonist and antagonist of CB1-receptor on penicillin-induced epileptiform activity in rats. Epilepsy Res. 2011; 93(2-3):128-37. 24. De Sousa DP, Gonçalves JCR, Quintans-Junior L. Study of anticonvulsant effect of citronellol, a monoterpene alcohol, in rodents. Neurosci Lett 2006; 401:231-5.25. Brito RG, Santos PL, Prado DS, et al. Citronellol reduces orofacial nociceptive behaviour in mice - evidence of involvement of retrosplenial cortex and periaqueductal grey areas. Basic Clin Pharmacol Toxicol. 2013;112(4):215-21.
Ayrıntılar
| Birincil Dil | İngilizce |
|---|---|
| Konular | Sağlık Kurumları Yönetimi |
| Bölüm | Makaleler |
| Yazarlar | |
| Yayımlanma Tarihi | 23 Ekim 2018 |
| Kabul Tarihi | 27 Ağustos 2018 |
| Yayımlandığı Sayı | Yıl 2018 Cilt: 10 Sayı: 3 |
