Genetic Variation in HIAT1 and IGF1R Genes in Some Goat Populations Reared in Türkiye

Year 2025, Volume: 65 Issue: 1, 8 - 14, 30.06.2025

Bedirhan Akbulut , Yusuf Aktaş , Veli Atmaca , Ebru Demir Nimet Tufan Eymen Demir Taki Karslı

https://doi.org/10.46897/livestockstudies.1722830

Abstract

This study aimed to assess whether genetic variants of the HIAT1 and IGF1R genes could be integrated into future marker-assisted selection (MAS) studies in three native Turkish goat populations known as Hair (HAI), Honamlı (HNM), and Kabakulak (KBK) to improve meat traits. In terms of the HIAT1 polymorphism, the frequency of the I allele ranged from 0.06 (KBK) to 0.35 (HNM), while the D allele frequency ranged from 0.65 (HNM) to 0.94 (KBK). No animals with the II genotype for the HIAT1 gene polymorphism were identified in KBK goats, while one animal carried this genotype in HAI goats. Regarding the IGFR1 gene polymorphism, the frequency of the A allele ranged from 0.72 (HAI) to 0.92 (HNM), while the T allele frequency varied between 0.08 (HNM) and 0.28 (HAI). Genetic distance-based phylogenetic tree separated HNM goats from KBK and HAI. The literature pointed out that animals with II and AT genotypes for the HIAT1 and IGF1R genes, respectively, are superior in terms of meat traits. Therefore, this study implies that the HIAT1 gene is promising for MAS studies in KBK goats, while IGF1R variation could be utilized to improve meat traits in all goat populations in the future.

Keywords

Genetic variation , Goat , PCR , Polymorphism

References

• Aktaş Y, Atmaca V, Demir E, Karslı T (2024). Assessment of InDel Variations in CPT1A and PRDM6 Genes Across Three Anatolian Goat Populations. Journal of Agricultural Faculty of Gaziosmanpaşa University, 41(3): 265-270.
• Alex P, Kanakkaparambil R, Gopalakrishnan R, Ramasamy C, Thazhathuveettil A (2023). The effect of insulin-like growth factor 1 receptor gene single nucleotide polymorphism on growth and milk production traits in two native Indian tropical goat breeds. Animal Biotechnology, 34(9): 4828-4836.
• Alkan H, Ugur T (2015). Views of hair goat breeders concerning the relationship between nomadic livestock and forestry: an example from the Turkey. International Journal of Environmental Research, 9(3): 969-976.
• Anh NTL, Kunhareang S, Duangjinda M (2015). Association of chicken growth hormones and insulin-like growth factor gene polymorphisms with growth performance and carcass traits in Thai broilers. Asian-Australasian Journal of Animal Sciences, 28(12): 1686-1695.
• Aslan M, Demir E, Karslı T (2022). Microsatellite diversity and restriction enzyme-based polymorphisms of MHC loci in some native Turkish goats. Journal of Agricultural Sciences, 28(4): 626-634.
• Dai L, Chen X, An D, Xiao M, Huang J, Xu J, Ruan Y (2024). The novel candidate gene Grb10 regulates the growth performance of Guizhou white goats. Small Ruminant Research, 234: 107267.
• Daskiran I, Savas T, Koyuncu M, Koluman N, Keskin M, Esenbuga N, Konyali A, Cemal İ, Gül S, Elmaz O, Kosum N, Dellal G, Bingöl M (2018). Goat production systems of Turkey: Nomadic to industrial. Small Ruminant Research, 163: 15-20.
• De la Rosa Reyna XF, Montoya HM, Castrellón VV, Rincón AMS, Bracamonte MP, Vera WA (2010). Polymorphisms in the IGF1 gene and their effect on growth traits in Mexican beef cattle. Genetics and Molecular Research, 9(2): 875-883.
• Degen AA (2007). Sheep and goat milk in pastoral societies. Small Ruminant Research, 68(1-2): 7-19.
• Demir E (2024). Microsatellite-based bottleneck analysis and migration events among four native Turkish goat breeds. Archives Animal Breeding, 67(3): 353-360.
• Ding N, Tian D, Li X, Zhang Z, Tian F, Liu S, Han B, Liu D, Zhao K (2022). Genetic polymorphisms of IGF1 and IGF1R genes and their effects on growth traits in Hulun Buir sheep. Genes, 13(4): 666.
• Elmaz Ö, Dağ B, Saatcı M, Aktaş HA, Mamak N, Gök B (2012). The determination of some morphological characteristics of Honamlı goat and kids, defined as a new indigenius goat breed of Turkey.
• FAO (2023). Food and Agriculture Organization of the United Nations, Food and Agriculture Data, Production, Crops and livestock products, Live Animals, Goats, https://www.fao.org/faostat/en/#data/QCL, last access: 29 March 2025.
• Gao J, Song X, Wu H, Tang Q, Wei Z, Wang X, Lan X, Zhang B (2020). Detection of rs665862918 (15-bp indel) of the HIAT1 gene and its strong genetic effects on growth traits in goats. Animals, 10(2): 358.
• Gezer G (2018). Antalya ili Elmalı, Kaş ve Muğla ili Fethiye yörelerinde yetiştirilen kıl keçilerinin bazı morfolojik özellikleri. Yüksek Lisans Tezi, Akdeniz Üniversitesi, Fen Bilimleri Enstitüsü, Antalya.
• Karslı BA, Demir E (2024). HAI, Honamlı ve Kabakulak Keçilerinde CMTM2 ve CSN1S1 Genlerinde Çoklu Doğumla İlişkili InDel Varyantların Belirlenmesi. Turkish Journal of Agriculture-Food Science and Technology, 12(9): 1577-1582.
• Karslı T,Atmaca V (2025). Identification of 21-bp indel polymorphism in the LLGL1 gene in several Turkish native goat populations. Mediterranean Agricultural Sciences, 38(1): 51-54.
• Karslı T, Demir E, Fidan HG, Aslan M, Karsli BA, Arik IZ, Sahin Semerci E, Karabag K, Balcioglu MS (2020). Determination of genetic variability, population structure and genetic differentiation of indigenous Turkish goat breeds based on SSR loci. Small Ruminant Research, 190: 106147.
• Lestari DA, Oikawa T, Sutopo S, Purbowati E, Setiaji A, Kurnianto E (2020). Effect of insulin-like growth factor 1 gene on growth traits of Kejobong goat and its growth analysis. Veterinary world, 13(1): 127-133.
• Luo J, Qin F, Deng C, Li F, Li W, Yue X (2019). Polymorphisms of IGF-IR gene and their association with economic traits in two indigenous Chinese dairy goat breeds. Gene, 695: 51-56.
• Luo Y, Akhatayeva Z, Mao C, Jiang F, Guo Z, Xu H, Lan X (2023). The ovine HIAT1 gene: mRNA expression, InDel mutations, and growth trait associations. Frontiers in Veterinary Science, 10: 1134903.
• Miller S, Dykes D, Plesky HA (1988). Simple Salting out Procedure for Extracting DNA from Human Cells. Nucleic Acids Research, 16(3): 1215-1215.
• Moaeen-ud-Din M, Danish Muner R, Khan MS (2022). Genome wide association study identifies novel candidate genes for growth and body conformation traits in goats. Scientific Reports, 12(1): 9891.
• Mohammadabadi M, Bordbar F, Jensen J, Du M, Guo W (2021). Key genes regulating skeletal muscle development and growth in farm animals. Animals, 11(3): 835.
• Pehlivan E (2019). Relationship between insulin-like growth factor-1 (IGF-1) concentrations and body trait measurements and climatic factors in prepubertal goat kids. Archives Animal Breeding, 62(1): 241-248.
• Tamura K, Stecher G, Kumar S (2021). MEGA11: molecular evolutionary genetics analysis version 11. Molecular Biology and Evolution, 38(7): 3022-3027.
• Wei Z, Wang K, Wu H, Wang Z, Pan C, Chen H, Lan X (2021). Detection of 15-bp deletion mutation within PLAG1 gene and its effects on growth traits in goats. Animals, 11(7): 2064.
• Xu L, Chen Z, Chen S, Chen Y, Guo J, Zhong T, Wang L, Zhan S, Li L, Zhang H, Cao J (2024). An identification of functional genetic variants in B4GALNT2 gene and their association with growth traits in goats. Genes, 15(3): 330.
• Yeh FC, Yang RC, Boiley T, Ye ZH, Mao JX (1997) Popgene, the user-friendly shareware for population genetic analysis. Canada: Molecular Biology and Biotechnology Center, University of Alberta.
• Zeder MA, Hesse B (2000). The initial domestication of goats (Capra hircus) in the Zagros mountains 10,000 years ago. Science, 287(5461): 2254-2257.