Obez bireylerde serum netrin-1 düzeyi ve obezite ile ilgili belirteçler arasındaki ilişki

Abstract

Netrinler, hem nöral hem de vasküler gelişime rehberlik eden önemli sinyal proteinleridir. Netrin-1 hücre çoğalması, yapışması ve göçü gibi birçok fizyolojik süreci düzenler. Bu çalışmanın amacı obez bireylerde serum netrin-1 düzeyi ile biyokimyasal parametreler arasındaki ilişkiyi belirlemektir. Obez (n=15) ve kontrol (n=30) bireylerden serum örnekleri alındı. Serum netrin-1 seviyeleri ELISA ile değerlendirildi. Değişken veriler Mann Whitney U testi ile karşılaştırıldı ve korelasyon Spearman korelasyon analizleri ile belirlendi. Obez grupta serumda netrin-1 protein seviyesinin kontrollere göre anlamlı derecede düşük olduğu belirlendi. Ortanca değer kontrol grubunda 844,0 pg/mL ve obez grupta 338 pg/mL idi (p<0,0001). Serum netrin-1 düzeyi HbA1c, LDL, kolesterol ve trigliserit ile negatif korelasyon gösterdi (P< 0.01). Ayrıca, ROC eğrisi analizi, netrin-1 seviyesinin 0.8667 AUC değeri ile obezite varlığını tanımlayabildiğini gösterdi (%95 Cl=0.7249-1.000; P<0.0001). Çalışmamız, netrin-1 salınımının obez bireylerde önemli ölçüde azaldığını ve obezite belirteçleri ile negatif korele olduğunu göstermektedir. Bu nedenle netrin-1, obezite için olası bir potansiyel biyobelirteç olarak değerlendirilebilir.

Keywords

• Obezite
• Netrin-1
• ELISA
• Biyobelirteç

Association between serum netrin-1 level and obesity-related markers in obese subjects

Abstract

Netrins are important signaling proteins that guide both neural and vascular development. Netrin-1 regulates many physiological processes such as cell proliferation, adhesion and migration. The aim of this study was to determine the association between serum netrin-1 level and biochemical parameters in obese subjects. Serum samples were collected from obese (n=15) and control (n=30) subjects. The serum netrin-1 levels were evaluated by ELISA. Variable data were compared by Mann Whitney U test and correlation was estimated by Spearman correlation analyses. The level of circulating netrin-1 protein was determined to be significantly lower in obese group compared to controls. The median value was 844.0 pg/mL in the control group and 338 pg/mL in the obese group (p<0.0001). Serum netrin-1 level was negatively correlated with HbA1c, LDL, cholesterol and triglyceride (P< 0.01). In addition, ROC curve analysis indicated that netrin-1 level could define the presence of obesity with AUC value of 0.8667 (95% Cl=0.7249-1.000; P<0.0001). Our study suggests that netrin-1 secretions are significantly reduced in obese subjects and negatively correlated with obesity markers. Therefore, netrin-1 can be evaluated as a possible potential biomarker for obesity.

Keywords

• Obesity
• Netrin-1
• ELISA
• Biomarker

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