Evaluation of Some Serum Acute Phase Proteins in Goats with Infectious Keratoconjunctivitis in Siirt Province

Abstract

The aim of this study was to evaluate certain acute phase proteins in goats with infectious keratoconjunctivitis. For this purpose, a total of 75 goats were used—50 with IKC and 25 healthy controls. After routine general and ophthalmic examinations (body temperature, pulse and respiration rate, lymph node palpation, abdominal palpation, and lung auscultation), eye swabs were collected from goats diagnosed with IKC. A total of 15 different bacteria were isolated from the eye swabs. The serum was separated from the blood samples taken from the goats included in the study and stored at -20 °C until analysis. Serum levels of amyloid A, C-reactive protein, ceruloplasmin, and fibrinogen were measured using commercial ELISA kits. Significant increases in acute phase protein levels were observed in the IKC group. Based on the results, it was suggested that acute phase proteins may be useful indicators for the diagnosis and monitoring of inflammation developing during IKC.

Keywords

• Goat
• Infectıous keratıconjunctivitis
• Acute Phase Proteins

Siirt İlinde Enfeksiyöz Keratokonjonktivitli Keçilerde Bazı Serum Akut Faz Proteinlerinin Değerlendirilmesi

Abstract

Bu çalışmanın amacı infeksiyöz keratokonjuktivit görülen keçilerde bazı akut faz proteinlerinin değerlendirilmesidir. Bu amaçla 50’si İKC’li ve 25’i sağlıklı olmak üzere toplam 75 keçi kullanıldı. Çalışmaya dahil edilen hayvanlara rutin genel muayene (beden sıcaklığı, nabız ve solunum sayısı, lenf düğümleri muayenesi, abdominal palpasyon, akciğer oskültasyonu) ve göz muayenesi yapıldıktan sonra, İKC olduğu belirlenen keçilerden göz svabı alındı. Alınan göz svaplarından toplam 15 farklı bakteri izole edildi. Çalışmaya dahil edilen keçilerden alınan kan örneklerinin serumları ayrıldı ve analiz edilinceye kadar -20 °C’de saklandı. Serum amiloid A, C-reaktif protein, seruloplazmin ve fibrinojenin serum seviyeleri ticari ELISA kitleriyle ölçüldü. İKC grubunda akut faz proteinlerinin düzeylerinde önemli artışlar gözlendi. Elde edilen sonuçlar doğrultusunda, İKC sırasında gelişen inflamasyonun tanı ve takibinde akut faz proteinlerinin önemli olabileceği düşünülmektedir.

Keywords

• Keçi
• İnfeksiyöz Keratokonjuktivit
• Akut Faz Protein

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