Research Article
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Effect of synthetic cannabinoids (JWH-018) on antibody response to HBV vaccination

Year 2021, , 21 - 26, 30.04.2021

Serkan Köksoy , Aynur Başalp

https://doi.org/10.24998/maeusabed.844896

Abstract

Synthetic cannabinoids can affect the immune system and can cause some changes in immune response. The immune response to the Hepatit B vaccine is complex. Studies on hepatitis B vaccine antibody response and JWH-018 are extremely limited. The main aim of this study was to investigate the effect of JWH-018 on anti-HBs Ag changes before or after Hepatitis B vaccination. The study was performed on C57BL6 mice (n=25). Mice were divided into 3 groups. Control Group; it was immunized Engerix B at 3 times 3-week intervals. Group 1; JWH-018 (1 mg/kg) was administered once a week for 4 weeks. At the end of this period, Engerix B was immunized 3 times at 3-week intervals. Group 2; it was immunized 3 times at 3-weeks intervals with Engerix B. At the end of this period JWH-018 (1 mg/kg) was treated once a week for 4 weeks. Blood samples (3 times with an interval of 2 weeks) were collected at the end of drug and vaccine administration. It was calculated that the means obtained from the control group were lower than the other groups. The final measurement of the within-group was higher than other measures and was statistically significant (p=0,017). Statistical difference was measured in the first (p=0,018) and third measurements (p=0,005) of the between groups. A total of 5 mice from the experimental groups died at different stages of the study. In this study, the use of JWH-018 has been shown to be effective on the anti-HBs parameter. We think that our study is very valuable in terms of proving the relationship between JWH-018 and Anti-Hbs parameter. However, more data are needed to understand causation.

Keywords

Hepatitis B Immunity JWH-018 Mice Vaccination

Supporting Institution

Burdur Mehmet Akif Ersoy University Scientific Research Projects Coordination Unit

Project Number

0423-YL-17

References

  • Agudelo, M., Newton, C., Widen, R., Sherwood, T., Nong, L., et al. 2008. Cannabinoid receptor 2 (CB2) mediates immunoglobulin class switching from IgM to IgE in cultures of murine-purified B lymphocytes. J Neuroimmunol., 3(1), 35–42. DOI:10.1007/s11481-007-9088-9
  • Aung, M.M., Griffin, G., Huffman, J.W., Wu, M., Keel, et al. 2000. Influence of the N-1 alkyl chain length of cannabimimetic indoles upon CB(1) and CB(2) receptor binding. Drug Alcohol Depend, 60(2), 133–140. DOI:10.1016/s0376-8716(99)00152-0
  • Chen, L., Zhang, Y., Zhang, S., Chen, Y., Shu, X., et al. 2019. A novel T-cell epitope in the transmembrane region of the hepatitis B virus envelope protein responds upon dendritic cell expansion. Arch Virol., 164(2), 483–495. https://doi.org/10.1007/s00705-018-4095-0
  • Croxford, J.L., & Yamamura, T. 2005. Cannabinoids and the immune system: potential for the treatment of inflammatory diseases?. J Neuroimmunol., 166(1-2),3–18. DOI:10.1016/j.jneuroim.2005.04.023
  • Do, Y., McKallip, R.J., Nagarkatti, M., Nagarkatti, P.S., 2004. Activation through cannabinoid receptors 1 and 2 on dendritic cells triggers NF-κB-dependent apoptosis: novel role for endogenous and exogenous cannabinoids in immunoregulation. J Neuroimmunol. 173(4):2373-2382. DOI: 10.4049/jimmunol.173.4.2373
  • Eisenstein, T.K., & Meissler, J.J., 2015. Effects of Cannabinoids on T-cell Function and Resistance to Infection. J Neuroimmune Pharmacol, 10(2),204–216. DOI:10.1007/s11481-015-9603-3
  • EMCDDA., 2010. Annual report on the state of the drugs problem in Europe, Lisbon.
  • Every-Palmer, S. 2011. Synthetic cannabinoid JWH-018 and psychosis: an explorative study. Drug Alcohol Depend, 117(2-3):152-157. DOI: 10.1016/j.drugalcdep.2011.01.012.
  • Filippelli, M., Lionetti, E., Gennaro, A., Lanzafame, A., Arrigo, T., et al. 2014. Hepatitis B vaccine by intradermal route in non responder patients: an update. World J Gastroenterol, 20(30), 10383–10394. DOI:10.3748/wjg.v20.i30.10383
  • Floreani, A., Baldo, V., Cristofoletti, M., Renzulli G., et al. 2004. Long-term persistence of anti-HBs after vaccination against HBV: an 18 year experience in health care workers. Vaccine., 22(5)6,607- 610. DOI: 10.1016/j.vaccine.2003.09.001
  • Hermanns-Clausen, M., Kneisel, S., Szabo, B., Auwärter, V. 2013. Acute toxicity due to the confirmed consumption of synthetic cannabinoids: clinical and laboratory findings. Addiction (Abingdon, England), 108(3):534–544. DOI:10.1111/j.1360-0443.2012.04078.x
  • Hou, J., Liu, Z., Gu, F. 2005. Epidemiology and prevention of hepatitis B virus infection. Int J Med Sci., 2(1),50-57. DOI:10.7150/ijms.2.50
  • Hwang, L., Y., Grimes, C.,Z., Tran, T.Q., Clark, A., et al. 2010. Accelerated hepatitis B vaccination schedule among drug users: a randomized controlled trial. The Journal of Infectious Diseases., 202(10),1500-1509. DOI: 10.1086/656776
  • Kakimi, K., Guidotti, L.G., Koezuka, Y., Chisari, F.V. (2000). Natural killer T cell activation inhibits hepatitis B virus replication in vivo. J Exp Med., 192(7), 921–930. DOI:10.1084/jem.192.7.921
  • Kiertscher, S.M., Gangalum, P.R., Ibrahim, G., Tashkin, D.P., Roth, M.D. 2018. A Prospective Study of Humoral and Cellular Immune Responses to Hepatitis B Vaccination in Habitual Marijuana Smokers. J Neuroimmune Pharmacol., 13(2), 219–229. DOI:10.1007/s11481-018-9776-7
  • Klein, T.W., Cabral, G.A. 2006. Cannabinoid-induced immune suppression and modulation of antigen-presenting cells. J Neuroimmune Pharmacol., 1(1),50–64. DOI:10.1007/s11481-005-9007-x
  • Koziel, M. J. (1998). The immunopathogenesis of HBV infection. Antivir Ther., 3(Suppl 3), 13–24. PMID: 10726052
  • Kuo, I., Sherman, S.G., Thomas, D.L., Strathdee, S.A. 2004. Hepatitis B virus infection and vaccination among young injection and non-injection drug users: missed opportunities to prevent infection. Drug Alcohol Depend., 73(1), 69–78. DOI:10.1016/j.drugalcdep.2003.10.007
  • Labay, L.M., Caruso, J.L., Gilson, T.P., Phipps, R.J., Knight, L. et al. 2016. Synthetic cannabinoid drug use as a cause or contributory cause of death. Forensic Sci Int, 260, 31–39. DOI: 10.1016/j.forsciint.2015.12.046
  • Lee, S. F., Newton, C., Widen, R., Friedman, H., Klein, T.W. 2001. Differential expression of cannabinoid CB(2) receptor mRNA in mouse immune cell subpopulations and following B cell stimulation. Eur J Pharmacol., 423(2-3), 235–241. https://doi.org/10.1016/s0014-2999(01)01122-0
  • Li, P., Tan, H., Xu, D., Yin, F., Cheng, Y., et al. 2014. Effect and mechanisms of curdlan sulfate on inhibiting HBV infection and acting as an HB vaccine adjuvant. Carbohydrate Polymers, 110, 446–455. DOI:10.1016/j.carbpol.2014.04.025
  • Marshell, R., Kearney-Ramos, T., Brents, L.K., Hyatt, W.S., Tai, S., et al. 2014. In vivo effects of synthetic cannabinoids JWH-018 and JWH-073 and phytocannabinoid Δ9-THC in mice: inhalation versus intraperitoneal injection. Pharmacology, Biochemistry, and Behavior, 124, 40–47. DOI:10.1016/j.pbb.2014.05.010
  • Marsland, A.L., Cohen, S., Rabin, B.S., Manuck, S.B. 2001. Associations between stress, trait negative affect, acute immune reactivity, and antibody response to hepatitis B injection in healthy young adults. Health Psychol., 20(1),4-11., PMID: 11199064.
  • Matsuda, L.A., Lolait, S.J., Brownstein, M.J., Young, A.C., Bonner, T.I. 1990. Structure of a cannabinoid receptor and functional expression of the cloned cDNA. Nature, 346(6284), 561–564. DOI:10.1038/346561a0
  • Milich, D.R., & Leroux-Roels, G. G. 2003. Immunogenetics of the response to HBsAg vaccination. Autoimmunity Reviews, 2(5),248–257. DOI:10.1016/s1568-9972(03)00031-4
  • Mir, A,, Obafemi, A., Young, A., Kane, C. 2011. Myocardial infarction associated with use of the synthetic cannabinoid K2. Pediatrics. 128(6):e1622-e1627. DOI:10.1542/peds.2010-3823
  • Munro, S., Thomas, K.L., Abu-Shaar, M. 1993. Molecular characterization of a peripheral receptor for cannabinoids. Nature, 365(6441), 61–65. DOI:10.1038/365061a0
  • Mutluay, D., Güngör, Ş., Tenekeci, G. Y., Köksoy, S., Çoban, C. S. 2019. Effects of synthetic (JWH-018) cannabinoids treatment on spermatogenesis and sperm function. Drug and Chemical Toxicology, 1–8. DOI:10.1080/01480545.2019.1680686
  • Ossato, A., Vigolo, A., Trapella, C., Seri, C., Rimondo, C., et al. 2015. JWH-018 impairs sensorimotor functions in mice. Neuroscience, 300, 174–188. DOI:10.1016/j.neuroscience.2015.05.021
  • Roth, M.D., Castaneda, J.T,, Kiertscher, S.M., 2015. Exposure to Δ9-tetrahydrocannabinol impairs the differentiation of human monocyte-derived dendritic cells and their capacity for T cell activation. J Neuroimmune Pharmacol., 10(2):333-343. DOI: 10.1007/s11481-015-9587-z
  • Showalter, V.M., Compton, D.R., Martin, B.R., Abood, M.E. 1996. Evaluation of binding in a transfected cell line expressing a peripheral cannabinoid receptor (CB2): identification of cannabinoid receptor subtype selective ligands. J Pharmacol Exp Ther., 278(3), 989–999.
  • Sleijffers, A., Garssen, J., de Gruijl, F. R., Boland, G. J., van Hattum, J., van Vloten, W. A., & van Loveren, H. 2002. UVB exposure impairs immune responses after hepatitis B vaccination in two different mouse strains. Photochem Photobiol, 75(5), 541–546. DOI:10.1562/0031-8655(2002)075<0541:ueiira>2.0.co;2
  • Samson, M.T., Small-Howard, A., Shimoda, L.M., Koblan-Huberson, M., et al. 2003. Differential roles of CB1 and CB2 cannabinoid receptors in mast cells. J Neuroimmunol. 170(10):4953-4962. DOI: 10.4049/jimmunol.170.10.4953.
  • Thanavala, Y., Mahoney, M., Pal, S., Scott, A., et al. 2005. Immunogenicity in humans of an edible vaccine for hepatitis B. Proc Natl Acad Sci., 102(9),3378-3382. DOI: 10.1073/pnas.0409899102
  • Wu, Y., Li, S., Li, H., Zhao, C., Ma, H., et al. 2016. Effect of a polysaccharide from Poria cocos on humoral response in mice immunized by H1N1 influenza and HBsAg vaccines. Int J Biol Macromol., 91, 248–257. DOI:10.1016/j.ijbiomac.2016.05.046

Year 2021, , 21 - 26, 30.04.2021

Serkan Köksoy , Aynur Başalp

https://doi.org/10.24998/maeusabed.844896

Abstract

Project Number

0423-YL-17

References

  • Agudelo, M., Newton, C., Widen, R., Sherwood, T., Nong, L., et al. 2008. Cannabinoid receptor 2 (CB2) mediates immunoglobulin class switching from IgM to IgE in cultures of murine-purified B lymphocytes. J Neuroimmunol., 3(1), 35–42. DOI:10.1007/s11481-007-9088-9
  • Aung, M.M., Griffin, G., Huffman, J.W., Wu, M., Keel, et al. 2000. Influence of the N-1 alkyl chain length of cannabimimetic indoles upon CB(1) and CB(2) receptor binding. Drug Alcohol Depend, 60(2), 133–140. DOI:10.1016/s0376-8716(99)00152-0
  • Chen, L., Zhang, Y., Zhang, S., Chen, Y., Shu, X., et al. 2019. A novel T-cell epitope in the transmembrane region of the hepatitis B virus envelope protein responds upon dendritic cell expansion. Arch Virol., 164(2), 483–495. https://doi.org/10.1007/s00705-018-4095-0
  • Croxford, J.L., & Yamamura, T. 2005. Cannabinoids and the immune system: potential for the treatment of inflammatory diseases?. J Neuroimmunol., 166(1-2),3–18. DOI:10.1016/j.jneuroim.2005.04.023
  • Do, Y., McKallip, R.J., Nagarkatti, M., Nagarkatti, P.S., 2004. Activation through cannabinoid receptors 1 and 2 on dendritic cells triggers NF-κB-dependent apoptosis: novel role for endogenous and exogenous cannabinoids in immunoregulation. J Neuroimmunol. 173(4):2373-2382. DOI: 10.4049/jimmunol.173.4.2373
  • Eisenstein, T.K., & Meissler, J.J., 2015. Effects of Cannabinoids on T-cell Function and Resistance to Infection. J Neuroimmune Pharmacol, 10(2),204–216. DOI:10.1007/s11481-015-9603-3
  • EMCDDA., 2010. Annual report on the state of the drugs problem in Europe, Lisbon.
  • Every-Palmer, S. 2011. Synthetic cannabinoid JWH-018 and psychosis: an explorative study. Drug Alcohol Depend, 117(2-3):152-157. DOI: 10.1016/j.drugalcdep.2011.01.012.
  • Filippelli, M., Lionetti, E., Gennaro, A., Lanzafame, A., Arrigo, T., et al. 2014. Hepatitis B vaccine by intradermal route in non responder patients: an update. World J Gastroenterol, 20(30), 10383–10394. DOI:10.3748/wjg.v20.i30.10383
  • Floreani, A., Baldo, V., Cristofoletti, M., Renzulli G., et al. 2004. Long-term persistence of anti-HBs after vaccination against HBV: an 18 year experience in health care workers. Vaccine., 22(5)6,607- 610. DOI: 10.1016/j.vaccine.2003.09.001
  • Hermanns-Clausen, M., Kneisel, S., Szabo, B., Auwärter, V. 2013. Acute toxicity due to the confirmed consumption of synthetic cannabinoids: clinical and laboratory findings. Addiction (Abingdon, England), 108(3):534–544. DOI:10.1111/j.1360-0443.2012.04078.x
  • Hou, J., Liu, Z., Gu, F. 2005. Epidemiology and prevention of hepatitis B virus infection. Int J Med Sci., 2(1),50-57. DOI:10.7150/ijms.2.50
  • Hwang, L., Y., Grimes, C.,Z., Tran, T.Q., Clark, A., et al. 2010. Accelerated hepatitis B vaccination schedule among drug users: a randomized controlled trial. The Journal of Infectious Diseases., 202(10),1500-1509. DOI: 10.1086/656776
  • Kakimi, K., Guidotti, L.G., Koezuka, Y., Chisari, F.V. (2000). Natural killer T cell activation inhibits hepatitis B virus replication in vivo. J Exp Med., 192(7), 921–930. DOI:10.1084/jem.192.7.921
  • Kiertscher, S.M., Gangalum, P.R., Ibrahim, G., Tashkin, D.P., Roth, M.D. 2018. A Prospective Study of Humoral and Cellular Immune Responses to Hepatitis B Vaccination in Habitual Marijuana Smokers. J Neuroimmune Pharmacol., 13(2), 219–229. DOI:10.1007/s11481-018-9776-7
  • Klein, T.W., Cabral, G.A. 2006. Cannabinoid-induced immune suppression and modulation of antigen-presenting cells. J Neuroimmune Pharmacol., 1(1),50–64. DOI:10.1007/s11481-005-9007-x
  • Koziel, M. J. (1998). The immunopathogenesis of HBV infection. Antivir Ther., 3(Suppl 3), 13–24. PMID: 10726052
  • Kuo, I., Sherman, S.G., Thomas, D.L., Strathdee, S.A. 2004. Hepatitis B virus infection and vaccination among young injection and non-injection drug users: missed opportunities to prevent infection. Drug Alcohol Depend., 73(1), 69–78. DOI:10.1016/j.drugalcdep.2003.10.007
  • Labay, L.M., Caruso, J.L., Gilson, T.P., Phipps, R.J., Knight, L. et al. 2016. Synthetic cannabinoid drug use as a cause or contributory cause of death. Forensic Sci Int, 260, 31–39. DOI: 10.1016/j.forsciint.2015.12.046
  • Lee, S. F., Newton, C., Widen, R., Friedman, H., Klein, T.W. 2001. Differential expression of cannabinoid CB(2) receptor mRNA in mouse immune cell subpopulations and following B cell stimulation. Eur J Pharmacol., 423(2-3), 235–241. https://doi.org/10.1016/s0014-2999(01)01122-0
  • Li, P., Tan, H., Xu, D., Yin, F., Cheng, Y., et al. 2014. Effect and mechanisms of curdlan sulfate on inhibiting HBV infection and acting as an HB vaccine adjuvant. Carbohydrate Polymers, 110, 446–455. DOI:10.1016/j.carbpol.2014.04.025
  • Marshell, R., Kearney-Ramos, T., Brents, L.K., Hyatt, W.S., Tai, S., et al. 2014. In vivo effects of synthetic cannabinoids JWH-018 and JWH-073 and phytocannabinoid Δ9-THC in mice: inhalation versus intraperitoneal injection. Pharmacology, Biochemistry, and Behavior, 124, 40–47. DOI:10.1016/j.pbb.2014.05.010
  • Marsland, A.L., Cohen, S., Rabin, B.S., Manuck, S.B. 2001. Associations between stress, trait negative affect, acute immune reactivity, and antibody response to hepatitis B injection in healthy young adults. Health Psychol., 20(1),4-11., PMID: 11199064.
  • Matsuda, L.A., Lolait, S.J., Brownstein, M.J., Young, A.C., Bonner, T.I. 1990. Structure of a cannabinoid receptor and functional expression of the cloned cDNA. Nature, 346(6284), 561–564. DOI:10.1038/346561a0
  • Milich, D.R., & Leroux-Roels, G. G. 2003. Immunogenetics of the response to HBsAg vaccination. Autoimmunity Reviews, 2(5),248–257. DOI:10.1016/s1568-9972(03)00031-4
  • Mir, A,, Obafemi, A., Young, A., Kane, C. 2011. Myocardial infarction associated with use of the synthetic cannabinoid K2. Pediatrics. 128(6):e1622-e1627. DOI:10.1542/peds.2010-3823
  • Munro, S., Thomas, K.L., Abu-Shaar, M. 1993. Molecular characterization of a peripheral receptor for cannabinoids. Nature, 365(6441), 61–65. DOI:10.1038/365061a0
  • Mutluay, D., Güngör, Ş., Tenekeci, G. Y., Köksoy, S., Çoban, C. S. 2019. Effects of synthetic (JWH-018) cannabinoids treatment on spermatogenesis and sperm function. Drug and Chemical Toxicology, 1–8. DOI:10.1080/01480545.2019.1680686
  • Ossato, A., Vigolo, A., Trapella, C., Seri, C., Rimondo, C., et al. 2015. JWH-018 impairs sensorimotor functions in mice. Neuroscience, 300, 174–188. DOI:10.1016/j.neuroscience.2015.05.021
  • Roth, M.D., Castaneda, J.T,, Kiertscher, S.M., 2015. Exposure to Δ9-tetrahydrocannabinol impairs the differentiation of human monocyte-derived dendritic cells and their capacity for T cell activation. J Neuroimmune Pharmacol., 10(2):333-343. DOI: 10.1007/s11481-015-9587-z
  • Showalter, V.M., Compton, D.R., Martin, B.R., Abood, M.E. 1996. Evaluation of binding in a transfected cell line expressing a peripheral cannabinoid receptor (CB2): identification of cannabinoid receptor subtype selective ligands. J Pharmacol Exp Ther., 278(3), 989–999.
  • Sleijffers, A., Garssen, J., de Gruijl, F. R., Boland, G. J., van Hattum, J., van Vloten, W. A., & van Loveren, H. 2002. UVB exposure impairs immune responses after hepatitis B vaccination in two different mouse strains. Photochem Photobiol, 75(5), 541–546. DOI:10.1562/0031-8655(2002)075<0541:ueiira>2.0.co;2
  • Samson, M.T., Small-Howard, A., Shimoda, L.M., Koblan-Huberson, M., et al. 2003. Differential roles of CB1 and CB2 cannabinoid receptors in mast cells. J Neuroimmunol. 170(10):4953-4962. DOI: 10.4049/jimmunol.170.10.4953.
  • Thanavala, Y., Mahoney, M., Pal, S., Scott, A., et al. 2005. Immunogenicity in humans of an edible vaccine for hepatitis B. Proc Natl Acad Sci., 102(9),3378-3382. DOI: 10.1073/pnas.0409899102
  • Wu, Y., Li, S., Li, H., Zhao, C., Ma, H., et al. 2016. Effect of a polysaccharide from Poria cocos on humoral response in mice immunized by H1N1 influenza and HBsAg vaccines. Int J Biol Macromol., 91, 248–257. DOI:10.1016/j.ijbiomac.2016.05.046
There are 35 citations in total.

Details

Primary Language English
Subjects Health Care Administration
Journal Section Research Article
Authors

Serkan Köksoy 0000-0001-5817-8213

Aynur Başalp 0000-0003-0604-6117

Project Number 0423-YL-17
Publication Date April 30, 2021
Submission Date December 30, 2020
Published in Issue Year 2021

Cite

APA Köksoy, S., & Başalp, A. (2021). Effect of synthetic cannabinoids (JWH-018) on antibody response to HBV vaccination. Mehmet Akif Ersoy University Journal of Health Sciences Institute, 9(1), 21-26. https://doi.org/10.24998/maeusabed.844896
AMA Köksoy S, Başalp A. Effect of synthetic cannabinoids (JWH-018) on antibody response to HBV vaccination. Mehmet Akif Ersoy University Journal of Health Sciences Institute. April 2021;9(1):21-26. doi:10.24998/maeusabed.844896
Chicago Köksoy, Serkan, and Aynur Başalp. “Effect of Synthetic Cannabinoids (JWH-018) on Antibody Response to HBV Vaccination”. Mehmet Akif Ersoy University Journal of Health Sciences Institute 9, no. 1 (April 2021): 21-26. https://doi.org/10.24998/maeusabed.844896.
EndNote Köksoy S, Başalp A (April 1, 2021) Effect of synthetic cannabinoids (JWH-018) on antibody response to HBV vaccination. Mehmet Akif Ersoy University Journal of Health Sciences Institute 9 1 21–26.
IEEE S. Köksoy and A. Başalp, “Effect of synthetic cannabinoids (JWH-018) on antibody response to HBV vaccination”, Mehmet Akif Ersoy University Journal of Health Sciences Institute, vol. 9, no. 1, pp. 21–26, 2021, doi: 10.24998/maeusabed.844896.
ISNAD Köksoy, Serkan - Başalp, Aynur. “Effect of Synthetic Cannabinoids (JWH-018) on Antibody Response to HBV Vaccination”. Mehmet Akif Ersoy University Journal of Health Sciences Institute 9/1 (April 2021), 21-26. https://doi.org/10.24998/maeusabed.844896.
JAMA Köksoy S, Başalp A. Effect of synthetic cannabinoids (JWH-018) on antibody response to HBV vaccination. Mehmet Akif Ersoy University Journal of Health Sciences Institute. 2021;9:21–26.
MLA Köksoy, Serkan and Aynur Başalp. “Effect of Synthetic Cannabinoids (JWH-018) on Antibody Response to HBV Vaccination”. Mehmet Akif Ersoy University Journal of Health Sciences Institute, vol. 9, no. 1, 2021, pp. 21-26, doi:10.24998/maeusabed.844896.
Vancouver Köksoy S, Başalp A. Effect of synthetic cannabinoids (JWH-018) on antibody response to HBV vaccination. Mehmet Akif Ersoy University Journal of Health Sciences Institute. 2021;9(1):21-6.