Investigation of Bovine Coronavirus and Bovine Rotavirus in Calves with Neonatal Diarrhea in Kırıkkale and Surrounding Provinces

Yıl 2021, Cilt: 9 Sayı: 3, 38 - 46, 22.12.2021

Emel AKSOY Erdal KARA Buğrahan Bekir YAGCI Ahmet Kürşat AZKUR

https://doi.org/10.24998/maeusabed.1010683

Cited By: 1

Öz

Sığır koronavirüs (BCoV) ve sığır rotavirüs (BRV) enfeksiyonları, sığır yetiştiriciliğindeki önemli sorunlardan biri olan neonatal buzağı ishallerinin en sık rastlanan viral etkenleri arasında yer almaktadır. Bu çalışmada, Kırıkkale ve çevre illerindeki neonatal ishalli 110 buzağıda BCoV ve BRV etkenleri RT-PCR ile araştırıldı ve pozitif BRV örnekleri PCR ile VP4 ve VP7 genleri temelli olarak genotiplendirildi. RT-PCR sonuçlarına göre toplamda 41 örnek BCoV pozitif (%37,27), 41 örnek BRV pozitif (%37,27) ve 20 örnek hem BCoV hem de BRV pozitif (%18,18) olarak belirlendi. Bu sonuçlara göre Kırıkkale, Kırşehir, Çankırı, Çorum, Yozgat ve Ankara illerinde yetiştirilen buzağılarda BCoV ve BRV, neonatal buzağı ishallerinin etkenleri olarak tespit edildi. BRV genotiplendirme sonuçlarına göre ise bu illerde ülkemizde yaygın olarak görülen genotipler olan G6P[5], G10P[5], G10P[11] ve G6P[11] genotiplerinin sirküle olduğu belirlendi. Bu genotiplerin buzağılarda saptanması, neonatal ishallere karşı aşılamanın ve aşılarda kullanılacak olan suş seçiminin önemini göstermektedir.

Anahtar Kelimeler

Kırıkkale, RT-PCR, Sığır koronavirüs, Sığır rotavirüs

Investigation of Bovine Coronavirus and Bovine Rotavirus in Calves with Neonatal Diarrhea in Kirikkale and Surrounding Provinces

Öz

Bovine coronavirus (BCoV) and bovine rotavirus (BRV) infections are very common in neonatal diarrhoea of calves which are of the important problems in cattle breeding. In the present study 110 calves with neonatal diarrhoea from Kirikkale and surrounding provinces were investigated for BCoV and BRV presence by RT-PCR in stool samples and positive BRV samples were genotyped by PCR based on VP4 and VP7 genes. In total, 41 samples were BCoV positive (37.27%) and 41 samples were BRV positive (37.27%), whereas 20 samples were positive for both BCoV and BRV (18.18%). According to the results of the study, BCoV and BRV are the neonatal calf diarrhoea agents in calves reared in Kirikkale, Kirsehir, Ankara, Cankiri, Corum, and Yozgat provinces. Genotyping results of positive BRV samples indicated that G6P[5], G10P[5], G10P[11], and G6P[11] genotypes, commonly seen in Turkey, are circulating among these provinces. Detection of these genotypes indicated importance of vaccination against neonatal diarrhoea and selection of vaccine strain.

Anahtar Kelimeler

Bovine coronavirus, Bovine rotavirus, Kirikkale, RT-PCR

Kaynakça

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