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The role of epidermal growth factor and cholinergic receptor agonists and antagonists in MAPK signal transduction in K562 cells

Yıl 2024, , 86 - 91, 28.01.2024
https://doi.org/10.5472/marumj.1379868

Öz

Objective: Muscarinic receptors (M1-M5) are members of the G protein-coupled receptor superfamily and are effective in physiological
functions through G proteins. Recent studies suggested that cholinergic receptors mediate cellular activities in hematopoietic cells.
The aim of this study was to investigate the potential role of mitogen-activated protein kinases (MAPK) signaling extracellular signalregulated
kinases 1 and 2 (ERK1/2)/phosphorylated ERK1/ (pERK1/2) pathways in chronic myeloid leukemia (K562) cells.
Materials and Methods: Chronic myeloid leukemia cells were cultured. Cells were incubated in the presence of muscarinic receptor
agonist, antagonist and epidermal growth factor (EGF). To detect MAPK activation, ERK/pERK protein expression levels were
determined by western blot method techniques.
Results: Our study results showed that cholinergic agents and EGFs affect the MAPK pathway in the human K562 cell line.
Conclusion: Cholinergic and EGF receptors may affect the MAPK pathway in K562 cells.
Conclusion: Cholinergic and EGF receptors may affect the MAPK pathway in K562 cells.

Kaynakça

  • Tansey EM. Henry Dale and the discovery of acetylcholine. C R Biologie 2006; 329:419-25. doi: 10.1016/j.crvi.2006.03.012
  • Fujii T, Mashimo M, Moriwaki Y, et al. Expression and function of the cholinergic system in immune cells. Front Immunol 2017;8: 1085. doi: 10.3389/fimmu.2017.01085ç.
  • Van Koppen CJ, Kaiser B. Regulation of muscarinic acetylcholine receptor signaling. Pharmacol Ther 2003; 98:197-220. doi: 10.1016/s0163-7258(03)00032-9.
  • Caulfield MP, Birdsall NJ. International Union of Pharmacology. XVII. Classification of muscarinic acetylcholine receptors. Pharmacol Rev 1998; 50:279-90.
  • Felder CC. Muscarinic acetylcholine receptors: Signal transduction through multiple effectors. FASEB J 1995 ;9; 619- 25.
  • Shah N, Khurana S, Cheng K, Raufman JP. Muscarinic receptors and ligands in cancer. Am J Physiol Cell Physiol 2009; 296: C221-C232. doi: 10.1152/ajpcell.005 14. 2008.
  • Nathanson MN. A multiplicity of muscarinic mechanisms: Enough signalling pathways to take your breath away. Proc Natl Acad Sci 2000 ;97, 6245-7. doi: 10.1073/pnas.97.12.6245
  • Eglen RM, Hedge SS, Watson N. Muscarinic receptor subtypes and smooth muscle function. Pharmacol Rev 1996; 48: 531- 65. PMID: 8981565.
  • Gutkind JS. Regulation of mitogen-activated protein kinase signaling networks by G protein-coupled receptors. Sci STKE. 2000 ;40:re1. doi: 10.1126/stke.2000.40.re1.
  • Park YS, Cho NJ. EGFR and PKC are involved in the activation of ERK1/2 and p90 RSK and the subsequent proliferation of SNU-407 colon cancer cells by muscarinic acetylcholine receptors. Mol Cell Biochem 2012 ;370:191-8. doi: 10.1007/ s11010.012.1410-z.
  • Ukegawa JI, Takeuchi Y, Kusayanagi S, Mitamura K. Growthpromoting effect of muscarinic acetylcholine receptors in colon cancer cells. J Cancer Res Clin Oncol 2003 ;129:272-8. doi: 10.1007/s00432.003.0433-y.
  • Kolch W. Meaningful relationships: The regulation of the Ras/ Raf/MEK/ERK pathway by protein interactions. Biochem J 2000; 351:289-305.
  • Rosenblum K, Futter M, Jones M, Hulme EC, Bliss TV. ERKI/II regulation by the muscarinic acetylcholine receptors in neurons. J Neurosci 2000; 20:977-85. doi: 10.1523/ JNEUROSCI.20-03-00977.2000
  • Cabadak H, Kucukibrahimoglu E, Aydin B, Kan B, Zafer Goren M. Muscarinic receptor-mediated nitric oxide release in a K562 erythroleukaemia cell line. Auton Autacoid Pharmacol. 2009; 29:109-15. doi: 10.1111/j.1474-8673.2009.00431.x
  • Cabadak H, Aydin B, Kan B. Regulation of M2, M3, and M4 muscarinic receptor expression in K562 chronic myelogenous leukemia cells by carbachol. J Recept Signal Transd Res 2011;31: 26-32. doi: 10.3109/10799.893.2010.506484.
  • Onder N, G, Aydin B, Cabadak, H. Studies on the role of alpha 7 nicotinic acetylcholine receptors in K562 cell proliferation and signaling. Mol Biol Rep 2021; 48: 5045-55. doi: 10.1007/ s11033.021.06498-4.
  • Liebmann C, Bohmer FD. Signal transduction pathways of G protein-coupled receptors and their cross-talk with receptor tyrosine kinases: Lessons from bradykinin signaling. Curr Med Chem 2000 ; 7: 911-43. doi: 10.2174/092.986.7003374589
  • Pearson G, Robinson F, Beers Gibson T, et al. Mitogenactivated protein (MAP) kinase pathways: Regulation and physiological functions. Endocr Rev 2001;22: 153-83. doi:10.1210/edrv.22.2.0428
  • Daub H, Wallasch C, Lankenau A, Herrlich A, Ullrich A. Signal characteristics of G protein-transactivated EGF receptor. EMBO J 1997 ;16: 7032-44. doi:10.1093/emboj/16.23.7032
  • Cheng K. Zimniak P. Raufman JP. Transactivation of the epidermal growth factor receptor mediates cholinergic agonist-induced proliferation of h508 human colon cancer cells. Cancer Res 2003 ;63: 6744-50.
  • Nicke B, Detjen K, Logsdon C. Muscarinic cholinergic receptors activate both inhibitory and stimulatory growth mechanisms in NIH3T3 cells. J Biol Chem 1999; 274: 21701-6. doi: 10.1074/jbc.274.31.21701
  • Kuol N, Davidson M. Karakkat J, et al. Blocking muscarinic receptor 3 attenuates tumor growth and decreases immunosuppressive and cholinergic markers in an orthotopic mouse model of colorectal cancer. Int J Mol Sci 2023; 24: 596. doi: 10.3390 /ijms24010596
  • Lanzafame A, Christopoulos A, Mitchelson F. Cellular signaling mechanisms for muscarinic acetylcholine receptors. Receptors Channels 2003; 9: 241-60.
  • Metzger M, Just L, Boss A, Drews U. Identification and functional characterization of the muscarinic receptor M3 in the human keratinocyte cell line haCat. Cells Tissues Organs 2005;180: 96-105. doi: 10.1159/000086750.
  • Prenzel N, Zwick E, Daub H, et al. EGF receptor transactivation by G-protein-coupled receptors requires metalloproteinase cleavage of proHB-EGF. Nature 1999; 402: 884-8. doi: 10.1038/47260
  • Yu H, Xia H, Tang Q, et al., Acetylcholine acts through M3 muscarinic receptor to activate the EGFR signaling and promotes gastric cancer cell proliferation. Sci Rep 2017; 7: 40802. doi: 10.1038/srep40802.
  • Yang T, He W, Cui F, et al. MACC1 mediates acetylcholineinduced invasion and migration by human gastric cancer cells. Oncotarget 2016; 7: 18085-94. doi: 10.18632/ oncotarget.7634.
  • Lan L, Wang H, Yang R, et al. R2-8018 reduces the proliferation and migration of non-small cell lung cancer cells by disturbing transactivation between M3R and EGFR. Life Sci 2019 ; 234: 116742. doi: 10.1016/j.lfs.2019.116742.
  • Calaf GM, Crispin LA, Muñoz JP, Aguayo F, Bleak TC. Muscarinic receptors associated with cancer. Cancers 2022;14:2322. doi:10.3390/cancers14092322
  • Kanlı Z, Cabadak H, Aydın B. Potential antiproliferative and apoptotic effects of pilocarpine combined with TNF alpha in chronic myeloid leukemia cells. Naunyn Schmiedebergs Arch Pharmacol 2023; 396: 1513-24 doi.org/10.1007/ s00210.023.02418-4 .
  • Kanlı Z, Aydın B, Cabadak H. Effects of several compounds in human erythroleukemia K562 cell proliferation and apoptosis. Marmara Med J 2019; 32:20-26. doi.org/10.5472/ marumj.518797
  • Aydin B, Tulunay A, Ekşioğlu-Demiralp E, Kan B, Cabadak H. Effects of carbachol on apoptosis in human chronic myelogenous leukemic K562 cell line. Marmara Med J 2019; 32: 38-43. doi.org 10.5472/marumj.518983.
Yıl 2024, , 86 - 91, 28.01.2024
https://doi.org/10.5472/marumj.1379868

Öz

Kaynakça

  • Tansey EM. Henry Dale and the discovery of acetylcholine. C R Biologie 2006; 329:419-25. doi: 10.1016/j.crvi.2006.03.012
  • Fujii T, Mashimo M, Moriwaki Y, et al. Expression and function of the cholinergic system in immune cells. Front Immunol 2017;8: 1085. doi: 10.3389/fimmu.2017.01085ç.
  • Van Koppen CJ, Kaiser B. Regulation of muscarinic acetylcholine receptor signaling. Pharmacol Ther 2003; 98:197-220. doi: 10.1016/s0163-7258(03)00032-9.
  • Caulfield MP, Birdsall NJ. International Union of Pharmacology. XVII. Classification of muscarinic acetylcholine receptors. Pharmacol Rev 1998; 50:279-90.
  • Felder CC. Muscarinic acetylcholine receptors: Signal transduction through multiple effectors. FASEB J 1995 ;9; 619- 25.
  • Shah N, Khurana S, Cheng K, Raufman JP. Muscarinic receptors and ligands in cancer. Am J Physiol Cell Physiol 2009; 296: C221-C232. doi: 10.1152/ajpcell.005 14. 2008.
  • Nathanson MN. A multiplicity of muscarinic mechanisms: Enough signalling pathways to take your breath away. Proc Natl Acad Sci 2000 ;97, 6245-7. doi: 10.1073/pnas.97.12.6245
  • Eglen RM, Hedge SS, Watson N. Muscarinic receptor subtypes and smooth muscle function. Pharmacol Rev 1996; 48: 531- 65. PMID: 8981565.
  • Gutkind JS. Regulation of mitogen-activated protein kinase signaling networks by G protein-coupled receptors. Sci STKE. 2000 ;40:re1. doi: 10.1126/stke.2000.40.re1.
  • Park YS, Cho NJ. EGFR and PKC are involved in the activation of ERK1/2 and p90 RSK and the subsequent proliferation of SNU-407 colon cancer cells by muscarinic acetylcholine receptors. Mol Cell Biochem 2012 ;370:191-8. doi: 10.1007/ s11010.012.1410-z.
  • Ukegawa JI, Takeuchi Y, Kusayanagi S, Mitamura K. Growthpromoting effect of muscarinic acetylcholine receptors in colon cancer cells. J Cancer Res Clin Oncol 2003 ;129:272-8. doi: 10.1007/s00432.003.0433-y.
  • Kolch W. Meaningful relationships: The regulation of the Ras/ Raf/MEK/ERK pathway by protein interactions. Biochem J 2000; 351:289-305.
  • Rosenblum K, Futter M, Jones M, Hulme EC, Bliss TV. ERKI/II regulation by the muscarinic acetylcholine receptors in neurons. J Neurosci 2000; 20:977-85. doi: 10.1523/ JNEUROSCI.20-03-00977.2000
  • Cabadak H, Kucukibrahimoglu E, Aydin B, Kan B, Zafer Goren M. Muscarinic receptor-mediated nitric oxide release in a K562 erythroleukaemia cell line. Auton Autacoid Pharmacol. 2009; 29:109-15. doi: 10.1111/j.1474-8673.2009.00431.x
  • Cabadak H, Aydin B, Kan B. Regulation of M2, M3, and M4 muscarinic receptor expression in K562 chronic myelogenous leukemia cells by carbachol. J Recept Signal Transd Res 2011;31: 26-32. doi: 10.3109/10799.893.2010.506484.
  • Onder N, G, Aydin B, Cabadak, H. Studies on the role of alpha 7 nicotinic acetylcholine receptors in K562 cell proliferation and signaling. Mol Biol Rep 2021; 48: 5045-55. doi: 10.1007/ s11033.021.06498-4.
  • Liebmann C, Bohmer FD. Signal transduction pathways of G protein-coupled receptors and their cross-talk with receptor tyrosine kinases: Lessons from bradykinin signaling. Curr Med Chem 2000 ; 7: 911-43. doi: 10.2174/092.986.7003374589
  • Pearson G, Robinson F, Beers Gibson T, et al. Mitogenactivated protein (MAP) kinase pathways: Regulation and physiological functions. Endocr Rev 2001;22: 153-83. doi:10.1210/edrv.22.2.0428
  • Daub H, Wallasch C, Lankenau A, Herrlich A, Ullrich A. Signal characteristics of G protein-transactivated EGF receptor. EMBO J 1997 ;16: 7032-44. doi:10.1093/emboj/16.23.7032
  • Cheng K. Zimniak P. Raufman JP. Transactivation of the epidermal growth factor receptor mediates cholinergic agonist-induced proliferation of h508 human colon cancer cells. Cancer Res 2003 ;63: 6744-50.
  • Nicke B, Detjen K, Logsdon C. Muscarinic cholinergic receptors activate both inhibitory and stimulatory growth mechanisms in NIH3T3 cells. J Biol Chem 1999; 274: 21701-6. doi: 10.1074/jbc.274.31.21701
  • Kuol N, Davidson M. Karakkat J, et al. Blocking muscarinic receptor 3 attenuates tumor growth and decreases immunosuppressive and cholinergic markers in an orthotopic mouse model of colorectal cancer. Int J Mol Sci 2023; 24: 596. doi: 10.3390 /ijms24010596
  • Lanzafame A, Christopoulos A, Mitchelson F. Cellular signaling mechanisms for muscarinic acetylcholine receptors. Receptors Channels 2003; 9: 241-60.
  • Metzger M, Just L, Boss A, Drews U. Identification and functional characterization of the muscarinic receptor M3 in the human keratinocyte cell line haCat. Cells Tissues Organs 2005;180: 96-105. doi: 10.1159/000086750.
  • Prenzel N, Zwick E, Daub H, et al. EGF receptor transactivation by G-protein-coupled receptors requires metalloproteinase cleavage of proHB-EGF. Nature 1999; 402: 884-8. doi: 10.1038/47260
  • Yu H, Xia H, Tang Q, et al., Acetylcholine acts through M3 muscarinic receptor to activate the EGFR signaling and promotes gastric cancer cell proliferation. Sci Rep 2017; 7: 40802. doi: 10.1038/srep40802.
  • Yang T, He W, Cui F, et al. MACC1 mediates acetylcholineinduced invasion and migration by human gastric cancer cells. Oncotarget 2016; 7: 18085-94. doi: 10.18632/ oncotarget.7634.
  • Lan L, Wang H, Yang R, et al. R2-8018 reduces the proliferation and migration of non-small cell lung cancer cells by disturbing transactivation between M3R and EGFR. Life Sci 2019 ; 234: 116742. doi: 10.1016/j.lfs.2019.116742.
  • Calaf GM, Crispin LA, Muñoz JP, Aguayo F, Bleak TC. Muscarinic receptors associated with cancer. Cancers 2022;14:2322. doi:10.3390/cancers14092322
  • Kanlı Z, Cabadak H, Aydın B. Potential antiproliferative and apoptotic effects of pilocarpine combined with TNF alpha in chronic myeloid leukemia cells. Naunyn Schmiedebergs Arch Pharmacol 2023; 396: 1513-24 doi.org/10.1007/ s00210.023.02418-4 .
  • Kanlı Z, Aydın B, Cabadak H. Effects of several compounds in human erythroleukemia K562 cell proliferation and apoptosis. Marmara Med J 2019; 32:20-26. doi.org/10.5472/ marumj.518797
  • Aydin B, Tulunay A, Ekşioğlu-Demiralp E, Kan B, Cabadak H. Effects of carbachol on apoptosis in human chronic myelogenous leukemic K562 cell line. Marmara Med J 2019; 32: 38-43. doi.org 10.5472/marumj.518983.
Toplam 32 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Cerrahi (Diğer)
Bölüm Original Research
Yazarlar

Selda Güler Atmaca 0000-0001-7369-3758

Banu Aydın 0000-0002-3267-8620

Hulya Cabadak 0000-0001-5757-2198

Yayımlanma Tarihi 28 Ocak 2024
Yayımlandığı Sayı Yıl 2024

Kaynak Göster

APA Güler Atmaca, S., Aydın, B., & Cabadak, H. (2024). The role of epidermal growth factor and cholinergic receptor agonists and antagonists in MAPK signal transduction in K562 cells. Marmara Medical Journal, 37(1), 86-91. https://doi.org/10.5472/marumj.1379868
AMA Güler Atmaca S, Aydın B, Cabadak H. The role of epidermal growth factor and cholinergic receptor agonists and antagonists in MAPK signal transduction in K562 cells. Marmara Med J. Ocak 2024;37(1):86-91. doi:10.5472/marumj.1379868
Chicago Güler Atmaca, Selda, Banu Aydın, ve Hulya Cabadak. “The Role of Epidermal Growth Factor and Cholinergic Receptor Agonists and Antagonists in MAPK Signal Transduction in K562 Cells”. Marmara Medical Journal 37, sy. 1 (Ocak 2024): 86-91. https://doi.org/10.5472/marumj.1379868.
EndNote Güler Atmaca S, Aydın B, Cabadak H (01 Ocak 2024) The role of epidermal growth factor and cholinergic receptor agonists and antagonists in MAPK signal transduction in K562 cells. Marmara Medical Journal 37 1 86–91.
IEEE S. Güler Atmaca, B. Aydın, ve H. Cabadak, “The role of epidermal growth factor and cholinergic receptor agonists and antagonists in MAPK signal transduction in K562 cells”, Marmara Med J, c. 37, sy. 1, ss. 86–91, 2024, doi: 10.5472/marumj.1379868.
ISNAD Güler Atmaca, Selda vd. “The Role of Epidermal Growth Factor and Cholinergic Receptor Agonists and Antagonists in MAPK Signal Transduction in K562 Cells”. Marmara Medical Journal 37/1 (Ocak 2024), 86-91. https://doi.org/10.5472/marumj.1379868.
JAMA Güler Atmaca S, Aydın B, Cabadak H. The role of epidermal growth factor and cholinergic receptor agonists and antagonists in MAPK signal transduction in K562 cells. Marmara Med J. 2024;37:86–91.
MLA Güler Atmaca, Selda vd. “The Role of Epidermal Growth Factor and Cholinergic Receptor Agonists and Antagonists in MAPK Signal Transduction in K562 Cells”. Marmara Medical Journal, c. 37, sy. 1, 2024, ss. 86-91, doi:10.5472/marumj.1379868.
Vancouver Güler Atmaca S, Aydın B, Cabadak H. The role of epidermal growth factor and cholinergic receptor agonists and antagonists in MAPK signal transduction in K562 cells. Marmara Med J. 2024;37(1):86-91.