Yıl 2019, Cilt 32 , Sayı 1, Sayfalar 14 - 19 2019-01-29

Yüksek yağlı diyetle indüklenmiş obez sıçanların bozulmuş epididimal sperm parametreleri üzerine egzersizin iyileştirici etkisi
Ameliorating effects of exercise on disrupted epididymal sperm parameters in high fat diet-induced obese rats

Merve AÇIKEL ELMAS [1] , Serap ARBAK [2] , Feriha ERCAN [3]


Amaç: Obezite erkek infertilite problemlerine neden olur ve sperm

kalitesini etkiler. Egzersizin erkek fertilitesi üzerine olumlu etkileri

olduğu çeşitli çalışmalar ile gösterilmiştir. Bu çalışmanın amacı,

yüksek yağlı diyetle (YYD) indüklenmiş obez sıçanlarda yüzme

egzersizinin epididimal sperm sayısı, motilitesi ve epididimis

morfolojisi üzerine etkilerini göstermektir.

Gereçler ve Yöntemler: Bu çalışmada dört deney grubu

(her grupta n=8) oluşturuldu. Standart (STD) ve STD+Egzersiz

(STD+EGZ) gruplarındaki sıçanlar standart sıçan diyeti ile

(kalorisinde %6 yağ içeren); YYD ve YYD+Egzersiz (YYD+EGZ)

grubundaki sıçanlar da yüksek yağlı diyet ile (kalorisinde %45 yağ

içeren) 18 hafta beslendiler. STD+EGZ ve YYD+EGZ grubundaki

sıçanlara deneyin son 6 haftasında haftada 5 gün, günde 1 saat yüzme

egzersizi yaptırıldı. Deney sonunda sol kaudal epididimis sperm

sayısı, motilitesi ve morfolojisinin incelenmesi için hazırlandı. Sağ

epididimis dokusu da histolojik inceleme için hazırlandı.

Bulgular: STD ve STD+EGZ gruplarında normospermik

parametreler gözlendi. STD grubu ile kıyaslandığında YYD

grubunda sperm sayısı ve motilitesinin anlamlı olarak azaldığı

ve morfolojik anomaliye sahip sperm sayısının arttığı gözlendi.

STD ve STD+EGZ gruplarında epididimal kanal spermatozoa

ile doluydu ve fibromusküler bağ dokusu düzenli morfolojide

gözlendi. YYD grubunda epididimal kanalların çoğu azalmış

sayıda spermatozoa kümesinden oluşuyordu. Ayrıca, epididimisin

stereosilyalı yalancı çok katlı silindirik epitelinde yer yer vakuol

oluşumları gözlendi. Egzersizin ise YYD ile indüklenmiş obez

sıçanlarda sperm parametrelerini ve epididimis kanalındaki sperm

kümesini arttırdığı gözlendi.

Sonuç: Bu çalışma, obezitenin sperm sayısını ve motilitesini

azalttığını, anomalili sperm sayısını arttırdığını ve epididimis

morfolojisini bozduğunu göstermektedir. Egzersizin ise, skrotal

ısıyı dengeleyerek ve epididimisteki olası oksidan hasarı

engelleyerek YYD ile indüklenmiş spermatogenik ve epididimal

hasarı iyileştirdiği düşünülmektedir.

Objective: Obesity causes male infertility problems and affects
the sperm quality. Recent studies have shown that exercise has
positive effects on male fertility. The present study aimed to show
the effects of swimming exercise on the epididymal sperm number,
motility and morphology in hight fat diet (HFD)-induced obese
rats.
Materials and Methods: Four experimental groups (n=8 in
each group) were formed. Standard (STD) and STD+Exercise
(STD+EXC) groups were fed with standard rat diet (6% of calories
as fat); HFD and HFD+Exercise (HFD+EXC) groups were fed
with high fat diet (45% of calories as fat) for 18 weeks. The rats
in STD+EXC and HFD+EXC groups were trained by swimming
sessions (1 h per day for 5 days a week) during the last 6 weeks of
the experiment. The left caudal epididymis was prepared to evaluate
the number, motility and morphology of the spermatozoa. The right
epididymal samples were processed for histological evaluation.
Results: Normospermic parameters were seen in STD and
STD+EXC groups. Sperm number and motility decreased and
spermatozoa with abnormal morphology increased significantly in
HFD group when compared with STD group. A large number of
spermatozoa in the epididymal duct lumen and regular morphology
of the fibromuscular connective tissue were observed in STD
and STD+EXC groups. Most of the epididymal ducts consisted
of decreased amount of spermatozoal accumulation in the HFD
group. Degenerated pseudostratified columnar epithelium with
vacuole formation were additional findings in this group. On the
other hand, swimming exercise had an enhancement effect on
sperm parameters with prominent spermatozoal accumulation in
the ducts of epididymis in HFD induced obese rats.
Conclusion: This study shows that HFD-induced obesity
decreased the number and motility of spermatozoa, increased
abnormal spermatozoa and caused disrupted epididymal
morphology. We hypothesize that exercise enhanced HFD-induced
spermatogenic and epididymal damages by the regulation of scrotal
heat and possible inhibition of oxidative damage in the epididymis.
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Birincil Dil en
Konular Tıp
Bölüm Makaleler
Yazarlar

Yazar: Merve AÇIKEL ELMAS

Yazar: Serap ARBAK

Yazar: Feriha ERCAN (Sorumlu Yazar)

Tarihler

Yayımlanma Tarihi : 29 Ocak 2019

Bibtex @araştırma makalesi { marumj518732, journal = {Marmara Medical Journal}, issn = {1019-1941}, eissn = {1309-9469}, address = {}, publisher = {Marmara Üniversitesi}, year = {2019}, volume = {32}, pages = {14 - 19}, doi = {10.5472/marumj.518732}, title = {Ameliorating effects of exercise on disrupted epididymal sperm parameters in high fat diet-induced obese rats}, key = {cite}, author = {AÇIKEL ELMAS, Merve and ARBAK, Serap and ERCAN, Feriha} }
APA AÇIKEL ELMAS, M , ARBAK, S , ERCAN, F . (2019). Ameliorating effects of exercise on disrupted epididymal sperm parameters in high fat diet-induced obese rats. Marmara Medical Journal , 32 (1) , 14-19 . DOI: 10.5472/marumj.518732
MLA AÇIKEL ELMAS, M , ARBAK, S , ERCAN, F . "Ameliorating effects of exercise on disrupted epididymal sperm parameters in high fat diet-induced obese rats". Marmara Medical Journal 32 (2019 ): 14-19 <https://dergipark.org.tr/tr/pub/marumj/issue/42918/518732>
Chicago AÇIKEL ELMAS, M , ARBAK, S , ERCAN, F . "Ameliorating effects of exercise on disrupted epididymal sperm parameters in high fat diet-induced obese rats". Marmara Medical Journal 32 (2019 ): 14-19
RIS TY - JOUR T1 - Ameliorating effects of exercise on disrupted epididymal sperm parameters in high fat diet-induced obese rats AU - Merve AÇIKEL ELMAS , Serap ARBAK , Feriha ERCAN Y1 - 2019 PY - 2019 N1 - doi: 10.5472/marumj.518732 DO - 10.5472/marumj.518732 T2 - Marmara Medical Journal JF - Journal JO - JOR SP - 14 EP - 19 VL - 32 IS - 1 SN - 1019-1941-1309-9469 M3 - doi: 10.5472/marumj.518732 UR - https://doi.org/10.5472/marumj.518732 Y2 - 2018 ER -
EndNote %0 Marmara Medical Journal Ameliorating effects of exercise on disrupted epididymal sperm parameters in high fat diet-induced obese rats %A Merve AÇIKEL ELMAS , Serap ARBAK , Feriha ERCAN %T Ameliorating effects of exercise on disrupted epididymal sperm parameters in high fat diet-induced obese rats %D 2019 %J Marmara Medical Journal %P 1019-1941-1309-9469 %V 32 %N 1 %R doi: 10.5472/marumj.518732 %U 10.5472/marumj.518732
ISNAD AÇIKEL ELMAS, Merve , ARBAK, Serap , ERCAN, Feriha . "Ameliorating effects of exercise on disrupted epididymal sperm parameters in high fat diet-induced obese rats". Marmara Medical Journal 32 / 1 (Ocak 2019): 14-19 . https://doi.org/10.5472/marumj.518732
AMA AÇIKEL ELMAS M , ARBAK S , ERCAN F . Ameliorating effects of exercise on disrupted epididymal sperm parameters in high fat diet-induced obese rats. Marmara Med J. 2019; 32(1): 14-19.
Vancouver AÇIKEL ELMAS M , ARBAK S , ERCAN F . Ameliorating effects of exercise on disrupted epididymal sperm parameters in high fat diet-induced obese rats. Marmara Medical Journal. 2019; 32(1): 19-14.