The effects of melatonin on the striatum

Yıl 2021, Cilt: 34 Sayı: 3, 234 - 340, 27.10.2021

Sinem Gergın Ozlem Kırazlı Hatice Boracı Sercan Dogukan Yıldız Umit Suleyman Sehırlı

https://doi.org/10.5472/marumj.1012077

Öz

Objective: Some of the neurological diseases cause morphologic changes in the striatal neurons. Medial forebrain bundle (MFB)
lesion is a commonly used method to produce a Parkinsonian model rat. Melatonin is a hormone which exerts a neuroprotective
effect on the neurons. The aim of this study is to investigate the effect of melatonin on the dendritic morphology of striatal medium
spiny neurons (MSNs) in rats with MFB lesion.
Materials and Methods: Twelve male Wistar albino rats were given saline injections into the MFB and divided into sedentary and
treatment groups. The treatment group was administered a 10 mg/kg dose of melatonin intraperitoneally for 30 days. The lesion
was confirmed histologically by Nissl staining. Golgi staining technique was applied to observe neuronal morphology. Neuronal
structures were analysed from three-dimensional images by Neurolucida (MBF Bioscience) software.
Results: The MFB lesion caused a reduction in the total dendritic length and in the number of dendritic endings. The melatonin
enhanced the number of dendritic endings compared to the sedentary group. The melatonin led to an increase in the total spine
density, spine densities of thin and mushroom types.
Conclusion: Melatonin improved the dendritic degeneration due to MFB lesion.

Anahtar Kelimeler

Dendritic spine, Melatonin, Golgi, Neurolucida

Kaynakça

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