Melatonin protects against acrylamide- induced oxidative tissue damage in rats

Year 2012, Volume: 16 Issue: 3, 213 - 221, 07.03.2014

Ayfer Tozan-beceren Ahmet Şehirli Emel Eksioglu-demiralp Göksel Şener Gülden Omurtag

Abstract

Melatonin protects against acrylamideinduced oxidative tissue damage in rats

Abstract

ABSTRACT: Acrylamide (ACR), is a widely used industrial chemical which induces oxidative
stress in the body. In this study we aimed to investigate the possible protective effect of
melatonin (MEL), as an antioxidant agent, against experimental ACR toxicity in the liver and
kidney of the rats. Wistar albino rats of either sex 200-250 g were divided into four groups
each consisting of 6 animals. Rats received for 10 days; 1) 0.9% NaCl i.p.; control (C) group;
2) MEL in a dose of 10 mg/kg i.p., 3) ACR in a dose of 40 mg/kg i.p. (dissolved in 0.9% NaCl)
(ACR group) and 4) MEL (in a dose of 10 mg/kg i.p.) and ACR (in a dose of 40 mg/kg i.p.).
After decapitation, liver and kidney tissues were excised. Malondialdehyde (MDA), glutathione
(GSH) levels, collagen contents and myeloperoxidase activity (MPO) were determined
in the tissues, while enzyme activities and cytokine levels were assayed in blood
samples. In the ACR treated group, GSH levels decreased significantly while the MDA levels,
MPO activity and collagen content increased in the tissues suggesting oxidative organ
damage. In the MEL treated ACR group, all of these oxidant responses were reversed significantly.
Serum enzyme activities, cytokine levels and leukocyte apoptosis which increased
significantly following ACR administration, decreased with MEL treatment. The results demonstrate
the role of oxidative mechanisms in ACR-induced tissue damage, and melatonin, by
its antioxidant properties, ameliorates oxidative organ injury due to acrylamide toxicity.
KEY WORDS: Acrylamide, melatonin, lipid peroxidation, glutathione, myeloperoxidase

Keywords

Acrylamide, melatonin, lipid peroxidation, glutathione, myeloperoxidase

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