Quality control evaluation and acetylcholinesterase inhibitory activity of Galanthus woronowii Losinsk.

Yıl 2011, Cilt: 15 Sayı: 2, 48 - 51, 07.03.2014

Ahmet Emir Nehir Ünver Somer Gülen Kaya , Buket Sarıkaya Mustafa Önür

Öz

ABSTRACT: Aerial and underground parts of Galanthus woronowii Losinsk., a wild growing
species in north-eastern Anatolia, were collected during flowering period. Quality control
and acetylcholinesterase inhibitory activity determinations were carried out on Bulbus and
Herba Galanthi prepared from plants collected from two different localities. In the context of
quality control studies, contents of humidity, total ash, sulphated ash, acid-insoluble ash
and total alkaloids of the drug specimens were determined and found to range between
8.463-9.343 %, 6.950-14.947 %, 9.743-17.930 %, 1.102-3.565 % and 0.247-0.499 %, respectively.
Additionally, acetylcholinesterase inhibitory activity of the alkaloidal extracts prepared from
the drug specimens were determined by using Thin Layer Chromatography (TLC) combined
with a bioautographic assay based on in vitro Ellman method. All of the alkaloidal extracts
displayed acetylcholinesterase inhibitory activity.
KEY WORDS: Galanthus woronowii, Amaryllidaceae, Quality control determination, Acetylcholinesterase
inhibitory activity

Anahtar Kelimeler

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Quality control evaluation and acetylcholinesterase inhibitory activity of Galanthus woronowii Losinsk

Öz

Kuzeydoğu Anadolu’da doğal olarak yetişen Galanthus woronowii Losinsk.’in toprak üstü ve toprak altı kısımları bitki çiçekli dönemde iken toplanmıştır. İki farklı lokaliteden toplanan bitkilerden hazırlanan Bulbus ve Herba Galanthi drogları üzerinde kalite kontrol ve asetilkolinesteraz inhibitör aktivite tayinleri gerçekleştirilmiştir. Kalite kontrol çalışmaları kapsamında drog örneklerinin nem, total kül, sülfat külü, hidroklorik asitte çözünmeyen kül ve total alkaloit içerikleri saptanmış ve bunların sırasıyla % 8.463-9.343, % 6.950-14.947, % 9.743-17.930, % 1.102-3.565 ve % 0.247-0.499 arasında değiştiği bulunmuştur. İlaveten, drog örneklerinden hazırlanan alkaloit ekstrelerinin asetilkolinesteraz inhibitör aktiviteleri in vitro Ellman yöntemine dayalı biyootografik bir deney ile kombine edilen İnce Tabaka Kromatografisinden (İTK) yararlanılarak tespit edilmiştir. Bütün alkaloit ekstreleri asetilkolinesteraz inhibitör aktivite göstermiştir

Anahtar Kelimeler

ANAHTAR KELİMELER: Galanthus woronowii, Amaryllidaceae, Kalite kontrol tayini, Asetilkolinesteraz inhibitör aktivite

Kaynakça

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