Spectral Analysis of Sargassum ilicifolium (Turner) C.Agardh and in vitro Anti-proliferative Study of its Ethanolic Extract and Chloroform Fraction Against Colon Cancer (HT-29) and Lung Cancer (A549) Cell Lines

Yıl 2017, Cilt: 21 Sayı: 2, 269 - 277, 01.04.2017

Mohan Sumithra V Chitra Radika Ramasamy

https://doi.org/10.12991/marupj.300349

Öz

Spectral Analysis of Sargassum ilicifolium (Turner) C. Agardh

and in vitro Anti-proliferative Study of its Ethanolic Extract and

Chloroform Fraction Against Colon Cancer (HT-29) and Lung

Cancer (A549) Cell Lines.

Current research in drug discovery from medicinal plants

involves the identification of safe and inexpensive lead molecule

towards diseases. Cancer is a second major national burden

leading to cause death. Reducing the nation’s cancer burden is

a great challenge to win the “War of cancer”. During the last

decades, numerous novel compounds have been found from

marine organisms with interesting pharmaceutical activities.

Hence, a novel brown algae, Sargassum ilicifolium (Turner)

C.Agardh belonging to Sargassaceae family has selected and

the study was aimed to investigate the in vitro anti-proliferative

activity of ethanolic extract and chloroform fraction of

Sargassum ilicifolium (Turner) C.Agardh. against colon cancer

(HT-29) and lung cancer (A549) cell lines. Extraction of seaweed

using 70% ethanol and fractionated with various solvents. Based

on phytochemical screening, chloroform fraction was selected,

then it was subjected to GC-MS analysis. The 70% ethanolic

exract and chloroform fraction were selected to in vitro MTT

cell line assay using colon cancer cell line (HT-29) and lung

cancer cell line (A549). The GC-MS analysis report confirmed

the presence of 21 compounds and in the the invitro assay,

IC50value showed the effective anti-proliferative activity against

colon and lung cancer cell lines. The results of the present study

proved the anti-proliferative effect of Sargassum ilicifolium.

Further, the active biomolecule in the fraction has to be isolated

and charecterized the formulated product subjected to in vivo

study to strengthen the anticancer activity.

Anahtar Kelimeler

Antiproliferative effect, colon cancer, lung cancer, Sargassum ilicifolium, GC-MS analysis

Sargassum ilicifolium (Turner) C.Agardh’ın Spektral Analizi ve Kolon Kanseri (HT-29) ve Akciğer Kanseri (A549) Hücre Serilerine Karşı Etanolik Ekstre ve Kloroform Fraksiyonunun in vitro Anti-proliferatif Çalışması

Öz

Tıbbi bitkilerden ilaç keşfi ile ilgili güncel araştırmalar,

hastalıklara karşı güvenli ve ucuz öncü molekül tanımlanmasını

gerektirir. Kanser ölüme neden olan ikinci büyük ulusal bir

yüktür. “Kanser Savaşı” nı kazanmak için ulusun kanser

yükünü azaltmak, büyük bir mücadeledir. Son on yılda,

deniz organizmalarından ilginç farmasötik aktiviteleri ile çok

sayıda yeni bileşik bulunmuştur. Bu nedenle, bu çalışmada

Sargassaceae familyasına ait yeni bir kahverengi yosun olan

Sargassum ilicifolium (Turner) C.Agardh seçilmiş ve Sargassum

ilicifolium (Turner) C.Agardh’ın etanolik ekstraktı ve kloroform

fraksiyonunun kolon kanseri (HT-29) ve akciğer kanseri (A549)

hücre serilerine karşı in vitro anti-proliferatif etkinliğinin

araştırılması amaçlamıştır. Deniz yosununun ekstraksiyonu

%70 etanol ve çeşitli çözücüler kullanarak fraksiyonlanmıştır.

Fitokimyasal taramaya dayanılarak, kloroform fraksiyonu

seçildi, sonra GC-MS analizine tabi tutuldu. İn vitro MTT hücre

hattı deneyi için, kolon kanseri hücre hattı (HT-29) ve akciğer

kanseri hücre hattı (A549) kullanılarak % 70 etanolik ekstrakt

ve kloroform fraksiyonu seçildi. GC-MS analizi raporunda 21

bileşiğin varlığı doğrulandı ve in vitro kolon ve akciğer kanseri

hücre hatlarına karşı anti-proliferatif aktivite etkisi IC50

değeri ile gösterildi. İleride fraksiyondaki aktif biyomolekül

izole edilmeli ve antikanser aktivitesini güçlendirmek için in

vivo incelemeye tabi tutulan formüle edilmiş ürün karakterize

edilmelidir.

Anahtar Kelimeler

Antiproliferatif etki, kolon kanseri, akciğer kanseri, Sargassum ilicifolium, GC-MS analizi

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