GABAergic effects of some foods extracts via inhibition of GABA transaminase (GABA-T)

Sümeyye Şahin; Sahra Haas

doi:10.19127/mbsjohs.976658

EN

GABAergic effects of some foods extracts via inhibition of GABA transaminase (GABA-T)

Abstract

Objective: GABAergic system is a target for various groups of medications including sedatives, anxiolytics, muscle relaxants, antidepressants and antiepileptics. Several foods or food ingredients are able to affect the GABAergic system by the inhibition of the γ-aminobutyric acid (GABA) degrading enzymes including the GABA-transaminase and succinate semialdehyde dehydrogenase. The purpose of this study to investigate the inhibitory effects of tea (Camellia sinensis), coffee (Coffea arabica L.), peppermint (Mentha piperita L.), thyme (Thymus vulgaris L.), and cinnamon (Cinnamomum zeylanicum) on GABA degrading enzymes.
Methods: The inhibition of the GABA-T by aqueous extracts of tea (Camellia sinensis), coffee (Coffea arabica L.), peppermint (Mentha piperita L.), cinnamon (Cinnamomum zeylanicum), and thyme (Thymus vulgaris L.) was investigated using a fluorometric microplate enzyme assay. Dose-dependent inhibition of the GABA-degrading enzymes was attained by all the food extracts tested. For determination of the IC50 values of the extracts (± 95 % CI), a linear regression was performed using Origin® (Origin® 2015G von Origin Lab Corporation, Northampton, MA 01060 USA).
Results: The aqueous extract of black tea presented the strongest inhibitory activity with an IC50-value (half maximal inhibitory concentration) of 13.0 (11.0-15.3) µg/mL. The tested food extracts were successful in inhibiting the GABA-degrading enzymes even at low concentrations.
Conclusion: In conclusion, the selected food extracts could serve as natural inhibitors for GABA-degrading enzymes thus, they could increase the GABA concentration in the brain.

Keywords

γ-Amino butyric acid, GABA-transaminase, enzyme inhibition, black tea, thyme

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Details

Primary Language

English

Subjects

Health Care Administration

Journal Section

Research Article

Authors

Sümeyye Şahin ^*
0000-0002-9344-7690
Türkiye

Sahra Haas
0000-0002-5574-1522
Germany

Publication Date

December 31, 2021

Submission Date

August 3, 2021

Acceptance Date

December 13, 2021

Published in Issue

Year 2021 Volume: 7 Number: 3

DOI

https://doi.org/10.19127/mbsjohs.976658

IZ

https://izlik.org/JA98RE96HB

APA

Şahin, S., & Haas, S. (2021). GABAergic effects of some foods extracts via inhibition of GABA transaminase (GABA-T). Middle Black Sea Journal of Health Science, 7(3), 423-428. https://doi.org/10.19127/mbsjohs.976658

AMA

1.Şahin S, Haas S. GABAergic effects of some foods extracts via inhibition of GABA transaminase (GABA-T). Mid Blac Sea J Health Sci. 2021;7(3):423-428. doi:10.19127/mbsjohs.976658

Chicago

Şahin, Sümeyye, and Sahra Haas. 2021. “GABAergic Effects of Some Foods Extracts via Inhibition of GABA Transaminase (GABA-T)”. Middle Black Sea Journal of Health Science 7 (3): 423-28. https://doi.org/10.19127/mbsjohs.976658.

EndNote

Şahin S, Haas S (December 1, 2021) GABAergic effects of some foods extracts via inhibition of GABA transaminase (GABA-T). Middle Black Sea Journal of Health Science 7 3 423–428.

IEEE

[1]S. Şahin and S. Haas, “GABAergic effects of some foods extracts via inhibition of GABA transaminase (GABA-T)”, Mid Blac Sea J Health Sci, vol. 7, no. 3, pp. 423–428, Dec. 2021, doi: 10.19127/mbsjohs.976658.

ISNAD

Şahin, Sümeyye - Haas, Sahra. “GABAergic Effects of Some Foods Extracts via Inhibition of GABA Transaminase (GABA-T)”. Middle Black Sea Journal of Health Science 7/3 (December 1, 2021): 423-428. https://doi.org/10.19127/mbsjohs.976658.

JAMA

1.Şahin S, Haas S. GABAergic effects of some foods extracts via inhibition of GABA transaminase (GABA-T). Mid Blac Sea J Health Sci. 2021;7:423–428.

MLA

Şahin, Sümeyye, and Sahra Haas. “GABAergic Effects of Some Foods Extracts via Inhibition of GABA Transaminase (GABA-T)”. Middle Black Sea Journal of Health Science, vol. 7, no. 3, Dec. 2021, pp. 423-8, doi:10.19127/mbsjohs.976658.

Vancouver

1.Sümeyye Şahin, Sahra Haas. GABAergic effects of some foods extracts via inhibition of GABA transaminase (GABA-T). Mid Blac Sea J Health Sci. 2021 Dec. 1;7(3):423-8. doi:10.19127/mbsjohs.976658

GABAergic effects of some foods extracts via inhibition of GABA transaminase (GABA-T)

GABAergic effects of some foods extracts via inhibition of GABA transaminase (GABA-T)

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