Investigation of antimicrobial activities of some sideritis species

Year 2023, , 19 - 21, 01.04.2023

İbrahim Yıldırım , Rasih Felek

https://doi.org/10.29136/mediterranean.1100612

Abstract

This research has been conducted on tea grown in the western Mediterranean region and also on the consumption of Sideritis stricta and Sideritis condensate species, in order to investigate their antimicrobial activity against 6 pathogenic bacteria. The plants were collected and dried at room temperature in the flowering stage. The essential oil of the plants was obtained with a Clevenger apparatus by the hydrodistillation method. Antibacterial activities of the extracts were determined with the disc diffusion method. In this method, MIC of the extracts which showed antibacterial activity were determined with the microdilution method. Staphylococcus aureus ATCC 29213, Escherichia coli ATCC 25922, Enterococcus faecalis ATCC 29212, Pseudomonas aeruginosa ATCC 27853, Salmonella enteretidis RSK 95046, Klebsiella pneumoniae ATCC 700613 were used as test bacteria. The results were evaluated according to the Clinical and Laboratory Standards Institute (CLSI) criteria. Ampicillin and penicillin were used as the positive control antibiotics. It was determined that hot water extracts of both plants did not have antibacterial activity. The results of the statistical analysis showed that there was a significant difference (P<0.05) between the antimicrobial activities of the essential oil of Sideritis stricta and Sideritis condensate. It was found that there was no significant difference between the antimicrobial activity of Sideritis stricta and ampicillin used as a control antibiotic. On the other hand, the antimicrobial activity of Sideritis condensata was lower when compared to the control antibiotic.

Keywords

Sideritis stricta, Sideritis condensate, antimicrobial activity

References

• Akgül A (1993) Baharat bilimi ve teknolojisi. Gıda Teknolojisi Derneği Yayınları No: 15, Ankara.
• Andrews JM (2001) Determination of minimum inhibitory concentrations. Journal of Anticrobial Chemotherapy 48: 5-16.
• Baydar H (2005) Tıbbi, aromatik ve keyf bitkileri bilimi ve teknolojisi, Süleyman Demirel Üniversitesi Yayınları No: 51, Isparta.
• Baytop T (1999) Türkiye’de bitkiler ile tedavi geçmişte ve bugün, İstanbul Üniversitesi Yayınları No: 3255 Eczacılık Fakültesi Yayın No: 40, 480 ss, İstanbul.
• Bilgehan H (2002) Klinik mikrobiyolojik tanı, Fakülteler Kitapevi Barış Yayınları, İzmir.
• Çopuroğlu Ö (2013) Niğde yöresindeki bazı endemik bitki türlerinin antimikrobiyal aktiviteleri, Niğde Üniversitesi Fen Bilimleri Enstitüsü Biyoloji Ana Bilim Dalı Yüksek Lisans Tezi, Niğde.
• Davis PH, Mill RR, Tan K (1988) Flora of Turkey and The East Aegean Islands, Vol. 10, Edinburgh University Press. Edinburgh.
• Dorman HJD, Deans SG (2000) Antimicrobial agents from plants: antibacterial activity of plant volatilr oils. Journal of Applied Microbiology 88: 308-316.
• Düzgündeş O, Kesici T, Kavuncu O, Gürbüz F (1987) Araştırma ve Deneme Metotları (İstatistik II). Ankara Üniversitesi Ziraat Fakültesi Yayınları. No: 1021, Ankara.
• Ekim T, Koyuncu M, Erik S, Ilarslan R (1989) Türkiye’nin tehlike altındaki nadir ve endemik bitkileri, Türkiye Tabiatını Koruma Derneği Yayınları, Ankara.
• Ezoubeiri A, Gadhi CA, Fdil N, Benharref A, Jana M, Vanhaelen M (2005) İsolation and antimicrobial activity of two phenolic compounds from Pulicaria odora L. Journal of Ethnopharmacology 99: 287-292.
• Güner A, Özhatay N, Ekim T, Baser KHC (2000) Flora of Turkey, Volume 11, Edinburgh University Press. Edinburgh.
• Karanika MS, Komaitis M, Aggelis G (2001) Effect of aqueous extracts of some plants of Lamiaceae family on the growth of Yarrowia lipolytica. International Journal of Food Microbiology 64: 175-181.
• Kılıç T (2006) Isolation and biological activity of new and known Diterpenoids from Sideritis stricta Boiss. & Heldr. Molecules 11: 257-262.
• Marino M, Bersani A, Comi G (2011) İmpedance measuraments to study the antimicrobial activity of essential oils from Lamiaceae and Compositae. International Journal of Food Microbiology 34:27-31.
• Proestos C, Boziaris IS, Nychas GJE, Komaitis M (2006) Analysis of flavanoids and phenolic acids an Greek aromatic plants: İnvestigation of their antioxidant capacity and antimicrobial activity. Food Chemistry 95: 664-671.
• Rauha JP, Remes S, Heinonen M, Hopia A, Kahkönen M, Kujala T, Pihlaja K, Vuorela H, Voulera P (2000) Antimicrobial effects of Finnish plant extracts containing flavonoids and other phenolic compounds. International Journal of Food Microbiology 56: 3-12.
• Sağlıkoğlu G (2004) Antemis tinctorıa (sarı papatya) bitkisinden hazırlanan özütlerin antibakteriyal etkilerinin incelenmesi. Yüksek Lisans Tezi, Çanakkale Onsekiz Mart Üniversitesi Fen Bilimleri Enstitüsü Kimya Anabilim dalı, Çanakkale.
• Toroğlu S, Çenet M (2006) Tedavi amaçlı kullanılan bazı bitkilerin kullanım alanları ve antimikrobiyel aktivitelerinin belirlenmesi için kullanılan yöntemler. Kahramanmaraş Sütçü İmam Üniversitesi Fen ve Mühendislik Dergisi 9: 12-20.
• TSI (1991) TS 8882. Baharat, Çeşni Veren ve Tıbbi Bitkiler-Uçucu Yağ Tayini. Türk Standartları Enstitüsü.
• Uğur A, Varol Ö, Ceylan Ö (2005) Antibacterial of Sideritis curvidens and Sideritis lonata from Turkey. Pharmaceutical Biology 43:47-52.
• Zhu X, Chen B, Ma M, Luo X, Zhang F, Yao S, Wan Z, Yang D, Hang H (2004) Simultaneous analysis of theanine, chlorogenic acid, purinealkaloids and catechins in tea samples with the help ofmulti-dimension information of on-line high performanceliquid chromatography/electrospray-mass spectrometry. Journal of Pharmaceutical and Biomedical Analysis 34: 695-70.