Expression patterns of ROS responsive genes on B-stressed canola (Brassica napus ssp. oleifera L.) following selenium treatment

Abstract

Though mitigating effect of selenium (Se) on various abiotic stresses is apparent, the knowledge on B-stress is very scant. Therefore, this work attempted to reveal its effects on B-stressed canola (Brassica napus ssp. oleifera L.) plants. In the present study, foliar gene expression and amounts of major antioxidant enzymes were investigated under different concentrations of individual (B or Se) and combined (B + Se) applications. The individual applications (per L) included 1.5 g B, 3 g B, 3 mg Se and 6 mg Se, while the combined applications were 1.5 g B+3 mg Se, 1.5 g B+6 mg Se, 3 g B+3 mg Se and 3 g B+6 mg Se. Under the given treatments, APX, CAT, SOD, POD and GR genes were mostly downregulated while some also showed upregulation such as APX (1.5 g B+6 mg Se and 3 g B+6 mg Se), SOD (1.5 g B+3 mg Se), CAT (3 g B, 3 mg Se and 3 g B+6 mg Se) and POD (1.5 g B). Assaying also implicated that beneficial effect of Se might be associated with the presence of stressor; otherwise, Se itself might induce the antioxidant mechanism as stressor. This work provides the results of individual and combined effects of B and Se applications on antioxidant gene expressions and protein activities in canola.

Keywords

• Abiotic stress,Antioxidant enzyme,ROS,Expression profile,Canola

Bor stresine maruz bırakılmış kanola bitkilerinde (Brassica napus ssp. oleifera L.) selenyum uygulaması sonrası ROS tepki genlerinin ifadelerinin belirlenmesi

Abstract

Selenyumun (Se) çeşitli abiyotik stresler üzerindeki azaltıcı etkisi üzerine çalışmalar mevcut olmakla birlikte, B-stresi üzerindeki etkisi hakkında bilgi oldukça azdır. Bu nedenle, bu çalışmada B-stresine maruz bırakılmış kanola (Brassica napus ssp. oleifera L.) bitkileri üzerinde selenyumun etkilerinin ortaya konulması hedeflenmiştir. Ayrı ayrı (B veya Se) ve eş zamanlı (B + Se) uygulamaların belli başlı antioksidan enzimlerinin yapraklardaki gen ekspresyonu ve protein miktarları üzerine etkisi araştırılmıştır. Yapılan ayrı ayrı uygulamalar (litrede) 1.5 g B, 3 g B, 3 mg Se ve 6 mg Se'yi içerirken, eş zamanlı uygulamalar 1.5 g B + 3 mg Se, 1.5 g B + 6 mg Se, 3g B + 3 mg Se ve 3 g B + 6 mg Se içermektedir. Uygulamalar altında, APX, CAT, SOD, POD ve GR genlerinin çoğunlukla ifadeleri azalırken, APX (1.5 g B + 6 mg Se ve 3 g B + 6 mg Se), SOD (1.5 g B + 3 mg Se), CAT (3 g B, 3 mg Se ve 3 g B + 6 mg Se) ve POD (1.5 g B) gibi bazılarının ifadeleri ise artış göstermişlerdir. Bu çalışma Se' nin yararlı etkisinin ortamda herhangi bir stres etkeninin var olup-olmadığı ile ilişkili olabileceğini; aksi takdirde, Se' nin kendisinin doğrudan stres etkeni olarak antioksidan mekanizmayı indükleyebileceğini ortaya koymaktadır. Çalışmayla ayrıca, B ve Se uygulamalarının antioksidan gen ifadesi ve protein aktiviteleri üzerindeki eş zamanlı etkilerinin nasıl sonuçlandığı da gösterilmiştir.

Keywords

• ROS,Abiyotik stres,Antioksidan enzim,Gen ifadesi,Kanola

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