Polymorphisms in candidate genes associated with egg yield and quality in brown layer pure lines

Year 2020, Volume: 33 Issue: 3, 433 - 439, 28.12.2020

Taki Karslı , Eymen Demir , Hüseyin Göktuğ Fidan Bahar Argun Karslı , Mehmet Aslan Sedat Aktan , Serdar Kamanlı , Kemal Karabağ , Emine Şahin Semerci Murat Balcıoğlu

https://doi.org/10.29136/mediterranean.698996

Cited By: 1

https://izlik.org/JA24GK62AR

Abstract

The aim of the present study was to detect a total of 11 polymorphisms of 6 genes (OCX32, GHR, DRD, VIP, VIPR, MR) related to egg yield and quality in six brown layer lines namely Rhode Island Red-I (RIRI), Rhode Island Red-II (RIRII), Barred Rock-I (BARI), Barred Rock-II (BARII), Colombian Rock (COL) and Line-54 (L5-4) raised in Ankara Poultry Research Institute. A total of 208 samples belonging to RIRI (n= 32), RIRII (n= 32), BARI (n= 40), BARII (n= 32), COL (n= 32) and L-54 (n= 40) were genotyped by PCR-RFLP method. For this reason, a total of 11 polymorphic regions were amplified by PCR and then PCR products were digested with specific restriction endonucleases. Results of the present study showed that polymorphisms and adequate genetic variations were found in OCX32-ex4/NcoI, OCX32-ex2/HpyCH4IV, DRD2/BseGI, VIPR-1/HhaI, VIPR-2/TaqI and MR1C/MboI genes which previously reported to associated with egg quality and yield in different chicken lines. However, conducting the association analysis between these genes and egg yield and quality will be useful. On the contrary, it has been revealed that GHR-intron 2/HindIII, GHR-intron 5/NspI, VIP-501/VspI, DRD1/BseNI and VIP-I2/HinfI polymorphisms cannot be used to increase egg yield and quality due to no detection of desired genotypes or homozygous excess.

Keywords

chicken lines , candidate genes , egg yield , egg quality , PCR-RFLP

Project Number

FBA-2015-756

Kahverengi yumurtacı saf tavuk hatlarında yumurta verim ve kalitesi ile ilişkili aday genlerdeki polimorfizmler

https://izlik.org/JA24GK62AR

Abstract

Bu çalışmanın amacı Ankara Tavukçuluk Araştırma Enstitüsünde yetiştirilen Rhode Island Red-I (RIRI), Rhode Island Red-II (RIRII), Barred Rock-I (BARI), Barred Rock-II (BARII), Colombian Rock (COL) ve Line-54 (L-54) isimli altı kahverengi saf yumurtacı tavuk hattında yumurta verim ve kalitesi ile ilişkili olan 6 gende (OCX32, GHR, DRD, VIP, VIPR, MR) toplam 11 polimorfizmin belirlenmesidir. RIRI (n= 32), RIRII (n= 32), BARI (n= 40), BARII (n= 32), COL (n= 32) ve L-54 (n= 40) hatlarına ait toplam 208 örnek PCR-RFLP yöntemiyle genotiplendirilmiştir. Bu amaçla, toplam 11 polimorfik bölge PCR ile çoğaltılmış ve daha sonra PCR ürünleri özgün restriksiyon enzimleriyle kesilmiştir. Mevcut çalışmanın sonuçları daha önce farklı tavuk ırklarında yumurta verimi ve kalitesiyle ilişkili olduğu bildirilen OCX32-ex4/NcoI, OCX32-ex2/HpyCH4IV, DRD2/BseGI, VIPR-1/HhaI, VIPR-2/TaqI ve MR1C/MboI genlerinde polimorfizmin ve yeterli genetik varyasyonun bulunduğunu göstermiştir. Bununla birlikte yumurta verim ve kalite özellikleri ile bu genler arasında ilişki analizlerinin yapılması faydalı olacaktır. Aksine, GHR-intron 2/HindIII, GHR-intron 5/NspI, VIP-501/VspI, DRD1/BseNI ve VIP-I2/HinfI gen bölgelerinde istenilen genotiplerin tespit edilememesi ya da homozigot fazlalığından dolayı bu gen bölgelerinin kullanılamayacağı tespit edilmiştir.

Keywords

tavuk hatları , aday genler , yumurta verimi , yumurta kalitesi , PCR-RFLP

Supporting Institution

Akdeniz Üniversitesi Bilimsel Araştırma Projeleri Koordinasyon Birimi

Project Number

FBA-2015-756

Thanks

Bu çalışma Akdeniz Üniversitesi Bilimsel Araştırma Projeleri Koordinasyon Birimi tarafından FBA-2015-756 proje numarası ile desteklenmiştir. Ayrıca Ankara Tavukçuluk Araştırmaları Enstitüsü çalışanlarına kan örneklerini temin ettikleri için teşekkür ederiz.

References

• Abdi M, Seyedabadi H, Gorbani A (2014) Prolactin and NPY gene polymorphism and its associations with production and reproductive traits in west-Azarbaijan native chicken. Bulletin of Environment, Pharmacology and Life Sciences 3: 39-45.
• Akbay R, Yalçın S, Ceylan N, Orhan E (2000) Türkiye tavukçuluğunda gelişmeler ve hedefler. Türkiye Ziraat Mühendisliği 5. Teknik Kongresi, Cilt II, Ankara, s. 795-810.
• Çelebi Ş, Karaca H (2006) Yumurtanın besin değeri, kolesterol içeriği ve yumurtayı n-3 yağ asitleri bakımından zenginleştirmeye yönelik çalışmalar. Atatürk Üniversitesi Ziraat Fakültesi Dergisi 37: 257-265.
• Demir E, Karslı T, Fidan HG, Argun Karslı B, Aslan M, Aktan S, Kamanlı S, Karabağ K, Şahin Semerci, E, Balcıoğlu MS (2020) Polymorphisms in some candidate genes associated with egg yield and quality in five different white layer pure lines. Kafkas Üniversitesi Veteriner Fakültesi Dergisi 26: 735-741.
• Fulton JE, Soller M, Lund AR, Arango, Lipkin E (2012) Variation in the ovocalyxin-32 gene in commercial egg-laying chickens and its relationship with egg production and egg quality traits. Animal Genetics 43: 102-113.
• Göger H, Demirtaş ŞE, Yurtoğullari Ş, Taşdemir AN, Şenkal UE, Boyali B (2017) Breeding studies on pure lines at Poultry Research Institute. Tavukçuluk Araştırma Dergisi 14: 30-38.
• Karslı T, Balcıoğlu MS, Demir E, Fidan HG, Aslan M, Aktan S, Kamanlı S, Karabağ K, Şahin E (2017) Determination of polymorphisms in IGF-I and NPY candidate genes associated with egg yield in pure layers chicken lines reared in the Ankara Poultry Research Institute. Turkish Journal of Agriculture-Food Science and Technology 5: 1051-1056.
• Karslı T, Balcıoğlu MS (2019) Genetic characterization and population structure of six brown layer pure lines using microsatellite markers. Asian-Australasian Journal of Animal Sciences 32: 49-57.
• Karslı T, Fidan HG (2019) Assesment of genetic diversity and conservation priorities among five White Leghorn Lines based on SSR markers. Animal Science Papers and Reports 37: 311-322.
• Landi F, Calvani R, Tosato M, Martone AM, Picca A, Ortolani E, Savera G, Salini S, Ramaschi M, Bernabei R, Marzetti E (2017) Animal-derived protein consumption is associated with muscle mass and strength in community-dwellers: Results from the Milan Expo survey. Journal of Nutrition, Health and Aging 21: 1050-1056.
• Lewis PD, Gous RM (2006) Effect of final photoperiod and twenty-week body weight on sexual maturity and early egg production in broiler breeders. Poultry Science 85: 377-383.
• Li HF, Zhu WQ, Chen KW, Wu X, Tang QP, Gao YS (2008) Associations between GHR and IGF-1 Gene polymorphisms, and reproductive traits in Wenchang Chickens. Turkish Journal of Veterinary and Animal Science 32: 281-285.
• Li HF, Zhu WQ, Chen KW, Wu X, Tang QP, Gao YS, Song WT, Xu HL (2009) Polymorphism in NPY and IGF-I genes associate with reproductive traits in Wenchang chicken. African Journal of Biotechnology 8: 4744-4748.
• Li HF, Zhu WQ, Chen KW, Song W, Shu J, Han W (2010) Effects of the polymorphisms of GHR gene and IGF-1 gene on egg quality in Wenchang chicken. Research Journal of Poultry Science 3: 19-22.
• Li DY, Zhang L, Smith DG, Xu HL, Liu YP, Zhao XL, Wang Y, Zhu Q (2013) Genetic effects of melatonin receptor genes on chicken reproductive traits. Czech Journal of Animal Science 58: 58-64.
• Miller S, Dykes D, Plesky HA (1988) Simple salting out procedure for extracting DNA from human cells. Nucleic Acids Research 16: 1215.
• Ngu NT, Xuan NH, Vu CT, An NT, Dung TN, Nhan NTH (2015) Effects of genetic polymorphisms on egg production in indigenous Noi chicken. Journal of Experimental Biology and Agricultural Sciences 3: 487- 493.
• Uemoto Y, Suzuki C, Sato S, Ohtake T, Sasaki O, Takahashi H, Kobayashi E (2009) Polymorphism of the ovocalyxin-32 gene and its association with egg production traits in the chicken. Poultry Science 88: 2512-2517.
• Xu HP, Shen X, Zhou M, Fang M, Zeng H, Nie Q, Zhang X (2010) The genetic effects of the dopamine D1 receptor gene on chicken egg production and broodiness traits. BMC Genetics 11: 1-10.
• Xu HP, Zeng H, Zhang DX, Jia XL, Luo CL, Fang MX, Nie QH, Zhang QX (2011) Polymorphisms associated with egg number at 300 days of age in chickens. Genetics and Molecular Research 10: 2279-2289.
• Yeh FC, Yang RC, Boyle TBJ, Ye ZH, Mao JX (1997) “POPGENE, The user-friendly shareware for population genetic analysis”. Molecular Biology and Biotechnology Centre, University of Alberta, Canada.
• Zhou M, Du Y, Nie Q, Liang Y, Luo C, Zeng H, Zhang X (2010) Associations between polymorphisms in the chicken VIP gene, egg production and broody traits. British Poultry Science 51: 195-203.