Investigation of the relationship between STAT5A and MYF5 gene polymorphisms and milk yield in simental cattle breed

Abstract

In this study, it is aimed to investigate the relationship between STAT5A-AvaI and MYF5-TaqI polymorphisms with daily and total lactation milk yields in a total of 202 animals belonging to Simmental cattle breed. Amplificated by PCR technique, 215 bp length of STAT5A gene region was digested with AvaI restriction enzyme, while 512 bp length of MYF5 gene region was digested with TaqIenzyme. The relationship between the determined STAT5A-AvaI and MYF5-TaqI genotypes and milk yields was evaluated with One-Way Variance Analysis (ANOVA). It was determined that the examined Simmental cattle population was in Hardy-Weinberg (H-W) equilibrium in terms of STAT5A-AvaI and MYF5-TaqI polymorphisms. It was observed that cattle with CT genotype had higher milk yield in both first and second lactations than other genotypes for STAT5A-AvaI polymorphism. On the other hand, it was determined that there was no relationship between MYF5-TaqI polymorphism and milk yield. As a result, it is thought that the STAT5A-AvaI polymorphism could be used to improve daily and total lactation milk yields in Simmental cattle breed.

Keywords

• MYF5
• RFLP
• Simmental
• STAT5A
• Milk yield

Simental ırkı sığırlarda STAT5A ve MYF5 gen polimorfizmleri ile süt verimi arasındaki ilişkinin araştırılması

Abstract

Bu çalışmada Simental sığır ırkına ait toplam 202 hayvanda STAT5A-AvaI ve MYF5-TaqI polimorfizmleri ile günlük ve toplam süt verimi arasındaki ilişkinin araştırılması amaçlanmıştır. PZR tekniği ile çoğaltılan 215 bç uzunluğundaki STAT5A gen bölgesi AvaI restriksiyon enzimi ile kesilirken, 512 bç uzunluğundaki MYF5 gen bölgesi TaqI enzimi ile kesilmiştir. Belirlenen STAT5A-AvaI ve MYF5-TaqI genotipleri ile günlük ve laktasyon süt verimleri arasındaki ilişki Tek Yönlü Varyans Analizi (ANOVA) ile değerlendirilmiştir. Bu çalışmada incelenen Simental popülasyonunun STAT5A-AvaI ve MYF5-TaqI polimorfizmi yönünden Hardy-Weinberg (H-W) dengesinde olduğu belirlenmiştir. Çalışma sonunda incelenen Simental ırkı sağmal ineklerde STAT5A-AvaI polimorfizmi yönünden CT genotipli sığırların hem birinci hemde ikinci laktasyonda diğer genotiplere göre daha yüksek süt verimine sahip oldukları belirlenmiştir. Buna karşın bu çalışmada incelenen Simental ırkı ineklerde MYF5-TaqI polimorfizmi ile günlük ve toplam laktasyon süt verimleri arasında ilişki olmadığı belirlenmiştir. Sonuç olarak, STAT5A-AvaI polimorfizminin, Simental sığır ırkında günlük ve toplam laktasyon süt verimlerinin iyileştirilmesi amacıyla yapılacak ıslah çalışmalarında kullanılabilme potansiyeline sahip olduğu düşünülmektedir.

Keywords

• MYF5
• RFLP
• Simental
• STAT5A
• Süt verimi

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