In vitro and in vivo assessment of the sensitivity of some tangerine mutants to Alternaria alternata pv. citri
Abstract
Alternaria brown spot disease caused by the Alternaria alternata pv. citri factor affects the leaves,
branches and fruits of many types of tangerines and their hybrids. This disease
has caused serious declines in the plantation areas of Minneola tangelo which
is widely produced in the Mediterranean Region while at the same time it has
hindered the expansion of the area of cultivation of late maturing tangerines
like Fortune. The isolate used in this study was obtained from a commercial
orchard of ‘Minneola tangelo’ in the province of Adana, Turkey. The sequence
analysis of the application of this isolates shows that a matchup of 99% was
achieved with isolate AHS-467.6 on the scale of the IFS primer. The aim of the
study was to identify tolerant genotypes from the mutants bred form the
‘Fortune’ tangerine which is known to be sensitive to the brown spot disease of
citrus. The sensitivity of individual mutants was tested by using leaves under in vitro conditions. The disease was
inoculated to tolerant mutant varieties under in vivo conditions and the results registered. The results of the
study showed that 9 mutant varieties among the 110 individuals under study were
found to be tolerant against the disease.
References
- Azevedo FA, Polydoro DA, Bastianel M, Kupper KC, Stuart RM, Costa FP, Pio RM (2010) Response of different tangerine varieties and hybrids to in vitro and in vivo inoculation of Alternaria alternata. Revista Brasileira de Fruticultura 32: 1–8 (with abstract in English).
- Breto MP, Ruiz C, Pina JA, Asins MJ (2001) The diversification of citrus clementina Hort. ex Tan. a vegetatively propagated crop species. Molecular Phylogenetics and Evolution 21: 285–293.
- Canıhoş Y, Erkkılıç A, Timmer LW (1997) First report of Alternaria brown spot of Minneola tangelo in Turkey. Plant Disease 81: 1214.
- Canıhoş Y, Peever TL, Timmer LW (1999) Temperature, leaf wetness, and isolate effects on infection of Minneola tangelo leaves by Alternaria sp. Plant Disease 83: 429–433.
- Castro Caicedo BL, Leguizamon JE, Lopez JA (1994) La mancha foliar de los cı´tricos em la zona cafetera. Avances Te´ cnicos Cenicafe´ 198: 1–8.
- Dalkilic Z, Timmer LW, Gmitter FG, Jr. (2005) Linkage of an Alternaria disease resistance gene in mandarin hybrids with RAPD fragments. Journal of American Society Horticultural Science 130 (2): 191-195.
- Deng ZN, Gentile A, Nicolosi E, Domina F, Vardi A, Tribulato E (1995) Identification of in vivo and in vitro lemon mutants by RAPD markers. The Journal of Horticultural Science & Biotechnology 70: 117–126.
- Gardner JM, Kono Y, Chandler JL (1986). Biossay and host-selectivity of Alternaria citri toxins affecting rouge lemon and mandarins. Physiol. Mol. Plant Pathol. 29: 293–304.
- Herrera L (1992) La mancha parda de los cı´tricos en Cuba. Levante Agrı´cola 31: 49–50.
- Kiely TB (1964) Brown spot of Emperor mandarin. Agriculture Gazetta N.S.W. 75: 854–856.
- Kohmoto K, Akimitsu K, Otani H (1991) Correlation of resistance and susceptibility of citrus to Alternaria alternata with sensitivity to host-specific toxins. Phytopathology 81: 719–722.
- Liu Q, Zhou AD, Chai LJ, Zhou WJ, Yu KQ, Ding J, Xu J, Deng XX (2009) Transcriptome analysis of a spontaneous mutant in sweet orange [Citrus sinensis (L.) Osbeck] during fruit development. Journal of Experimental Botany 60: 801–813.
- Marin JE, Fernandez HS, Peres NA, Andrew M, Peever TL, Timmer LW (2006) First report of Alternaria Brown spot of citrus caused by Alternaria alternata in Peru. Phytopathology 90: 686.
- Nemsa I, Hernandez MA, Lacasa A, Porras I, Garcia-Lidon A, Cifuentes D, Bouzid S, Ortuno A, Del Rio JA (2012) Pathogenicity of Alternaria alternata on fruits and leaves of ‘Fortune’ mandarin (Citrus clementina x Citrus tangerina). Canadian Journal of Plant Pathology 34(2): 195-202.
- Peever TL, Olsen L, Ibáñez A, Timmer LW (2000) Genetic differentiation and host specificity among populations of Alternaria spp. causing brown spot of grapefruit and tangerine × grapefruit hybrids in Florida. Phytopathology 90: 407–414.
- Pegg KG (1966) Studies of a strain of Alternaria citri Pierce, the causal organism of brown spot of Emperor mandarin. Queensland Journal of Agricultural Animal Science 23: 14–18.
- Peres NAR, Agostini JP, Timmer LW (2003) Outbreaks of Alternaria brown spot of citrus in Brazil and Argentina. Plant Disease 87: 750.
- Schutte GC, Lesar KH, Pelser P du T, Swart SH (1992) The use of tebuconazole for the control of Alternaria alternata on ‘Minneola’ tangelos and its potential to control post-harvest decay when applied as a pre-harvest spray. Proceedings of the International Society of Citriculture 7: 1070–1074.
- Solel Z (1991) Alternaria brown spot on Minneola tangelos in Israel. Plant Pathology 40: 145–147.
- Solel Z, Kimchi M (1997) Susceptibility and resistance of citrus genotypes to Alternaria alternata pv. citri. Journal of Phytopathology 145: 389–391.
- Souza MC, Stuchi ES, Goes A (2009) Evaluation of tangerine hybrid resistance to Alternaria alternata. Scientia Horticulturae 123: 01–04.
- Stuart RM, Bastianel M, Azevedo FA, Machado MA (2009) Alternaria brown spot. Laranja 30: 29–44 (with abstract in English).
- Whiteside JO (1976) A newly recorded Alternaria-induced brown spot disease on Dancy tangerines in Florida. Plant Disease Reporter 60: 326–329.
In vitro ve in vivo koşullarında bazı mandarin mutantlarının Alternaria alternata pv. citri etmenine duyarlılıklarının değerlendirilmesi
Abstract
Alternaria alternata pv. citri etmeninin neden
olduğu Alternaria kahverengi leke hastalığı birçok mandarin ve melezlerinin
yaprak, dal ve meyvelerini etkiler. Söz konusu hastalık Akdeniz bölgesinde yetiştiriciliği yapılan Minneola tanjelonun dikim
alanlarında ciddi azalmalara neden olmuş
ve ayrıca yetiştiricilik açısından geççi bir çeşit olan
Fortune gibi mandarin çeşitlerinin gelişimini engellemiştir. Çalışmada kullanılan
izolat Adana ilinde bulunan ticari ‘Minneola tanjelo’ bahçelerinden elde
edilmiştir. Bu izolat sekans analizi sonucunda ITS1 primerine göre %99 oranında
AHS-467-6 izolatı ile eşleşme sağlamıştır. Mutasyon ıslahı ile turunçgillerde
kahverengi leke hastalığına hassas olduğu bilinen ‘Fortune’ mandarini
mutantları arasından tolerant genotiplerin elde edilmesi hedeflenmiştir. In vitro koşullarda koparılmış yapraklar
kullanılarak mutant bireylerin hastalığa karşı hassasiyetleri belirlenmiştir.
Ayrıca, tolerant olarak bulunan mutantların fidanlarına in vivo koşullarda hastalık inokulasyonu yapılarak sonuçlar kontrol
edilmiştir. Çalışma sonucunda 111 mutant birey arasından 9 adet mutant birey
hastalığa karşı tolerant olarak bulunmuştur.
References
- Azevedo FA, Polydoro DA, Bastianel M, Kupper KC, Stuart RM, Costa FP, Pio RM (2010) Response of different tangerine varieties and hybrids to in vitro and in vivo inoculation of Alternaria alternata. Revista Brasileira de Fruticultura 32: 1–8 (with abstract in English).
- Breto MP, Ruiz C, Pina JA, Asins MJ (2001) The diversification of citrus clementina Hort. ex Tan. a vegetatively propagated crop species. Molecular Phylogenetics and Evolution 21: 285–293.
- Canıhoş Y, Erkkılıç A, Timmer LW (1997) First report of Alternaria brown spot of Minneola tangelo in Turkey. Plant Disease 81: 1214.
- Canıhoş Y, Peever TL, Timmer LW (1999) Temperature, leaf wetness, and isolate effects on infection of Minneola tangelo leaves by Alternaria sp. Plant Disease 83: 429–433.
- Castro Caicedo BL, Leguizamon JE, Lopez JA (1994) La mancha foliar de los cı´tricos em la zona cafetera. Avances Te´ cnicos Cenicafe´ 198: 1–8.
- Dalkilic Z, Timmer LW, Gmitter FG, Jr. (2005) Linkage of an Alternaria disease resistance gene in mandarin hybrids with RAPD fragments. Journal of American Society Horticultural Science 130 (2): 191-195.
- Deng ZN, Gentile A, Nicolosi E, Domina F, Vardi A, Tribulato E (1995) Identification of in vivo and in vitro lemon mutants by RAPD markers. The Journal of Horticultural Science & Biotechnology 70: 117–126.
- Gardner JM, Kono Y, Chandler JL (1986). Biossay and host-selectivity of Alternaria citri toxins affecting rouge lemon and mandarins. Physiol. Mol. Plant Pathol. 29: 293–304.
- Herrera L (1992) La mancha parda de los cı´tricos en Cuba. Levante Agrı´cola 31: 49–50.
- Kiely TB (1964) Brown spot of Emperor mandarin. Agriculture Gazetta N.S.W. 75: 854–856.
- Kohmoto K, Akimitsu K, Otani H (1991) Correlation of resistance and susceptibility of citrus to Alternaria alternata with sensitivity to host-specific toxins. Phytopathology 81: 719–722.
- Liu Q, Zhou AD, Chai LJ, Zhou WJ, Yu KQ, Ding J, Xu J, Deng XX (2009) Transcriptome analysis of a spontaneous mutant in sweet orange [Citrus sinensis (L.) Osbeck] during fruit development. Journal of Experimental Botany 60: 801–813.
- Marin JE, Fernandez HS, Peres NA, Andrew M, Peever TL, Timmer LW (2006) First report of Alternaria Brown spot of citrus caused by Alternaria alternata in Peru. Phytopathology 90: 686.
- Nemsa I, Hernandez MA, Lacasa A, Porras I, Garcia-Lidon A, Cifuentes D, Bouzid S, Ortuno A, Del Rio JA (2012) Pathogenicity of Alternaria alternata on fruits and leaves of ‘Fortune’ mandarin (Citrus clementina x Citrus tangerina). Canadian Journal of Plant Pathology 34(2): 195-202.
- Peever TL, Olsen L, Ibáñez A, Timmer LW (2000) Genetic differentiation and host specificity among populations of Alternaria spp. causing brown spot of grapefruit and tangerine × grapefruit hybrids in Florida. Phytopathology 90: 407–414.
- Pegg KG (1966) Studies of a strain of Alternaria citri Pierce, the causal organism of brown spot of Emperor mandarin. Queensland Journal of Agricultural Animal Science 23: 14–18.
- Peres NAR, Agostini JP, Timmer LW (2003) Outbreaks of Alternaria brown spot of citrus in Brazil and Argentina. Plant Disease 87: 750.
- Schutte GC, Lesar KH, Pelser P du T, Swart SH (1992) The use of tebuconazole for the control of Alternaria alternata on ‘Minneola’ tangelos and its potential to control post-harvest decay when applied as a pre-harvest spray. Proceedings of the International Society of Citriculture 7: 1070–1074.
- Solel Z (1991) Alternaria brown spot on Minneola tangelos in Israel. Plant Pathology 40: 145–147.
- Solel Z, Kimchi M (1997) Susceptibility and resistance of citrus genotypes to Alternaria alternata pv. citri. Journal of Phytopathology 145: 389–391.
- Souza MC, Stuchi ES, Goes A (2009) Evaluation of tangerine hybrid resistance to Alternaria alternata. Scientia Horticulturae 123: 01–04.
- Stuart RM, Bastianel M, Azevedo FA, Machado MA (2009) Alternaria brown spot. Laranja 30: 29–44 (with abstract in English).
- Whiteside JO (1976) A newly recorded Alternaria-induced brown spot disease on Dancy tangerines in Florida. Plant Disease Reporter 60: 326–329.
Details
| Primary Language | English |
|---|---|
| Subjects | Agricultural Engineering |
| Journal Section | Makaleler |
| Authors | |
| Publication Date | August 1, 2019 |
| Submission Date | August 10, 2018 |
| Published in Issue | Year 2019 Volume: 32 Issue: 2 |
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