Markör destekli piramit potivirüs direnç genleri, Ruanda fasulye (Phaseolus vulgaris L.) genotipleri

Year 2019, Volume: 32 Issue: 3, 381 - 385, 01.12.2019

Charles Ruhımbana , Nedim Mutlu

https://doi.org/10.29136/mediterranean.580098

Cited By: 2

Abstract

Özellikle
Doğu ve Orta Afrika'da bulunan ve fasulye veriminde önemli bir tehdit olan
fasulye adi mozaik virüsü ve fasulye adi mozaik nekroz virüsü potirviruse
aittir. Dayanıklılığı arttırmak ve verimi stabilize etmek için dominant ve
resesif direnç genlerini birleştirmek virüsün bilinen tüm suşlarına karşı en
etkili hastalık kontrol stratejisidir. Çalışmanın amacı, hem dominant I hem de resesif bc-3 genlerini, marker destekli geri melezleme ıslahı yoluyla
hassas Ruanda fasulyelerine transfer etmektir. I geni (BCMV-48289723-CAPS) ve bc-3
geni (ENM-CAPS) için spesifik markerler BC progenlerinde seçilim için
kullanıldı. Başarılı gen kombinasyonu progenilerde BC1F1 ve BC2F1'in %33'ünde,
BC3F1'in %43'ünde ve BC3F2 %16'sında başarıyla gerçekleştirilmiştir. Tohum
rengi, bazı BC3 progenilerinde kombine direnç genleriyle tamamen geri
kazanılmıştır. Gelişmiş hatların, mahsulü Doğu ve Orta Afrika koşullarında hem
BCMV hem de BCMNV'ye karşı koruması beklenmektedir.

Keywords

Geri melezleme, Fasulye adi mozaik virüsü, Fasulye adi mozaik nekroz virüsü, Fasulye

Marker-assisted pyramiding potyvirus resistance genes into Rwandan common bean (Phaseolus vulgaris L.) genotypes

Abstract

Bean common mosaic virus and Bean common mosaic
necrosis virus belong to Potyvirus and the disease poses significant threat to
bean yield especially in East and Central Africa. Combining the dominant and
recessive resistance genes is the most effective disease control against all
known strains of the virus, a strategy to enhance the durability and stabilize
the yield. The aim of the study was to transfer both the dominant I and recessive bc-3 genes into susceptible Rwandan common beans via
marker-assisted backcross breeding. The markers specific for I gene (BCMV-48289723-CAPS) and for bc-3
gene (ENM-CAPS) were used for
selection in BC progenies. Successful gene combination was established
in 33% of BC1F1 and BC2F1, 43% of BC3F1 and 16% of BC3F2 progenies. The seed
color was fully recovered on some of the BC3 progenies with combined resistance
genes. The advanced lines are expected to shield the crop against both BCMV and
BCMNV under East and Central African conditions.

Keywords

Backcross (BC), Bean common mosaic virus (BCMV), Bean common mosaic necrosis virüs (BCMNV), Phaseolus vulgaris

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