Ameliorative effects of vitamin E on sperm morphological defects of cocks fed fumonisin B1 contaminated diets

Year 2021, Volume: 34 Issue: 2, 241 - 247, 02.08.2021

Olumuyiwa Joseph Olarotimi , Olufemi Adu , Olayinka Asolo , Dotun Olorunfemi Francis Gbore

https://doi.org/10.29136/mediterranean.809370

Abstract

The study investigated the ameliorative effect of vitamin E on testicular parameters, semen characteristics and sperm morphology of mature cocks fed Fumonisin B1 (FB1) contaminated diets. Twenty four weeks old 160 cocks were used for the 16 weeks experiment. The cocks were assigned to eight experimental diets, six diets contained varying inclusion levels of FB1 with and without the inclusion of vitamin E. Diets 2, 3, and 4 contained 10, 20, and 30 mg kg-1 FB1 respectively without vitamin E while Diets 6, 7, and 8 contained 10, 20, and 30 mg kg-1 FB1 respectively with 200 mg kg-1 vitamin E each. Diet 1 was the control without vitamin E while Diet 5 was the control with vitamin E. Each group was replicated four times with five cocks per replicate in a completely randomized design. There were significant (P<0.05) reductions in the left, right, and paired testicular weights and volumes as well as the paired epididymal weight of the cocks fed diets contaminated with varying levels of FB1 while the testicular densities were not significantly (P>0.05) influenced. The ejaculate volume, sperm motility, total sperm cells, total motile cells, mass activity, and total live cells of the cocks fed varying levels of FB1 were equally significantly (P<0.05) reduced. However, significant (P<0.05) increases were observed in the secondary sperm morphological abnormalities such as curved tails, headless tails, and rudimentary tails. Inclusion of vitamin E in the diets significantly (P<0.05) improved the affected parameters, especially, among the cocks fed 10 mg FB1/kg diet. Therefore, vitamin E is recommended as a feed additive to improve the reproductive potentials of cocks fed FB1-contaminated diets.

Keywords

Testes, Semen, Abnormality, Fumonisin, Ejaculate

Fumonisin B1 ile kontamine rasyonlarla beslenen horozların sperm morfolojik kusurları üzerine E vitamininin iyileştirici etkileri

Abstract

Bu çalışmada, E vitamini takviyesinin, Fumonisin B1 (FB1) kontamine diyetlerle beslenen erişkin horozların testis parametreleri, semen özellikleri ve sperm morfolojisi üzerindeki iyileştirici etkisi araştırılmıştır. Bu amaçla, 16 hafta süren deneme için 24 haftalık yaştaki 160 horoz kullanılmıştır. Materyal, farklı FB1 düzeyleri içeren altı rasyonun yanında, E vitamini içeren ve içermeyen rasyon olmak üzere sekiz gruba ayrılmışlardır. 2, 3 ve 4 nolu grup, E vitamini içermeyip sırasıyla 10, 20 ve 30 mg kg-1 FB1 içerirken, 6, 7 ve 8 nolu grup, 200 mg kg-1 E vitamininin yanında sırasıyla 10, 20 ve 30 mg kg-1 FB1 içermektedir. 1 nolu grup, E vitamini içermeyen kontrol grubu ve 5 nolu grup E vitamini içerikli kontrol grubudur. Hayvanlar her bir gruba, beş horozdan oluşan dörder tekerrür olacak şekilde şansa bağlı olarak dağıtılmışlardır. İnceleme sonucunda, farklı düzeylerde FB1 kontamine rasyonlarla beslenen horozların sol, sağ ve eşli testis ağırlıkları ve hacimlerinin yanı sıra eşli epididimal ağırlıklarında önemli (P<0.05) azalmalar olduğu, testis yoğunluklarındaki değişimin ise anlamlı olmadığı (P>0.05). ) görülmüştür. Ayrıca, farklı seviyelerde FB1 ile beslenen horozların ejakülat hacmi, sperm motilitesi, toplam sperm hücresi, toplam hareketli hücreler, kütle aktivitesi ve toplam canlı hücre sayısı önemli ölçüde azalmıştır (P<0.05). Bununla birlikte, kavisli kuyruk, başsız kuyruk ve ilkel kuyruk gibi ikincil sperm morfolojik anormalliklerinde önemli artışlar gözlenmiştir (P<0.05). Rasyonlara E vitamininin dahil edilmesi, özellikle 10 mg FB1/kg içeren rasyonla beslenen horozlarda, etkilenen parametreleri önemli ölçüde iyileştirmiştir (P<0.05). Bu nedenle, FB1 ile kontamine rasyonlarla beslenen horozların üreme potansiyellerini geliştirmek için yem katkı maddesi olarak E vitamini önerilmektedir.

Keywords

Testis, Semen, Anormallik, Fumonisin, Ejakülat

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