Molecular analysis of resistance gene locus to bacterial canker and wilting disease in tomato mutants
Abstract
The tomato plant is one of the most widely produced vegetables in the world. However, there are several disease factors which limit tomato production. The Clavibacter michiganensis subsp. michiganensis (Cmm) pathogen causes wilting and canker symptoms on the leaves of plants and bird’s eye symptoms on the tomato and then the plants completely die. Development of resistant tomato varieties is prerequisite due to absence of an effective control methods against the bacterial disease. The resistant M3-9 and M3-15 tomato plants have been developed because of mutation from susceptible NCEBR3 tomato seeds with ethyl methanosulfonate (EMS). For each chromosome of the tomato genome, 24 SSR markers were selected from each end of the haploid, 12 tomato chromosomes, and polymorphic differences between susceptible and resistant tomato plants were studied. Polymorphisms were found with SSR13 and SSRB18031 markers located on chromosome 5 with resistant mutants, M3-9, M3-15 and susceptible original NCEBR3 plants. It is envisaged that a resistance gene is located on the 5th chromosome of resistant M3-9 and M3-15 plants. Further fine mapping studies will reveal the location of the resistance gene(s) for controlling bacterial canker and wilting pathogen.
Keywords
Polymerase Chain Reaction SSR markers Maping Clavibacter michiganensis subsp. michiganensis
Supporting Institution
Gaziosmanpaşa Üniversitesi Bilimsel Projeler Koordinatörlüğü
Project Number
TOGUBAP 2013/117 No’lu Araştırma Projesi
References
- Agrios GN (2005) Plant Pathology. Elvesier Academic Press, London.
- Bayan Y (2011) Investigation of phenollics in resistant and susceptible tomatoes to bacterial canker disease (Clavibacter michiganensis subsp. michaganensis). MSc Thesis, Gaziosmanpasa University, Tokat.
- Çalış Ö, Topkaya Ş (2011) Genetic analysis of resistance to early blight disease in tomato. African Journal of Biotechnology 10(79):18071-18077.
- Çalış Ö, Saygı S, Çelik D, Bayan Y (2013) Resistance to bacterial canker and wilting disease of tomato and reverse genetics. Akdeniz University Journal of the Faculty of Agriculture 26(1): 5-10.
- Çalış Ö, Karabulut D, Karakaş H, Özdemir F (2015) Characterization of resistance in bacterial cancer and wilt disease resistant tomato genotypes. In: 2. Central Anatolia Region Agriculture and Food Congress, Nevşehir, Turkey, pp. 558-558.
- FAO (2021) Tomato production. http://www.faostat.fao.org. Accessed 24 February, 2021.
- Fermentas (2014) Genomic DNA purification kit K0512.www.fermentas.com. Accessed 23 January, 2014.
- Grant M, Lamb C (2006) Systemic immunity. Current Opinion in Plant Biology 9: 414-420.
- Lahaye T (2004) Illuminating the molecular basis of gene-for-gene resistance; Arabidopsis thaliana RRS1-R and its interaction with Ralstonia solanacearum popP2. Trends in Plant Science 9: 1-4.
- Meyers BC, Kaushik S, Nandety RS (2005) Evolving disease resistance genes. Current Opinion in Plant Biology 8: 129-134.
- Nash AF, Gardner RG (1988) Tomato early blight resistance in a breeding line derived from Lycopersicon hirsutum P. I. 126445. Plant Disease 72: 206-209.
- Oxoid (2013) Plate count agar tryptone glucose yeast agar protocol. http://www.oxoid.com/UK/blue/proddetail/proddetail.asp?pr=CM0325&cat=&sec=1. Accessed 18 November, 2013.
- Powell W, Machray GC, Provan J (1996) Polymorphism revealed by simple sequence repeats. Trends in Plant Science 1(7): 1360-1385.
- Rathjen JP, Moffett P (2003) Early signal transduction events in specific plant disease relocation. Nature Genetics 37: 777-782.
- Sambrook J, Fritsch EF, Maniatis T (1989) Molecular Cloning A Laboratory Manual. 2nd Edition, Cold Spring Harbor Laboratory Press, New York.
- Solgenomics (2013) Solanacea Genomics Network. http://solgenomics.net. Accessed 20 May, 2013.
- TÜİK (2021) Tomato production amount in Turkey. http://www.tuik.gov.tr. Accessed 24 February, 2021.
- Wiermer M, Feys BJ, Parker JE (2005) Plant immunity: the eds1 regulatory node. Current Opinion in Plant Biology 8: 383-389.
Molecular analysis of resistance gene locus to bacterial canker and wilting disease in tomato mutants
Abstract
The tomato plant is one of the most widely produced vegetables in the world. However, there are several disease factors which limit tomato production. The Clavibacter michiganensis subsp. michiganensis (Cmm) pathogen causes wilting and canker symptoms on the leaves of plants and bird’s eye symptoms on the tomato and then the plants completely die. Development of resistant tomato varieties is prerequisite due to absence of an effective control methods against the bacterial disease. The resistant M3-9 and M3-15 tomato plants have been developed because of mutation from susceptible NCEBR3 tomato seeds with ethyl methanosulfonate (EMS). For each chromosome of the tomato genome, 24 SSR markers were selected from each end of the haploid, 12 tomato chromosomes, and polymorphic differences between susceptible and resistant tomato plants were studied. Polymorphisms were found with SSR13 and SSRB18031 markers located on chromosome 5 with resistant mutants, M3-9, M3-15 and susceptible original NCEBR3 plants. It is envisaged that a resistance gene is located on the 5th chromosome of resistant M3-9 and M3-15 plants. Further fine mapping studies will reveal the location of the resistance gene(s) for controlling bacterial canker and wilting pathogen.
Keywords
Polymerase Chain Reaction SSR markers Maping Clavibacter michiganensis subsp. michiganensis
Project Number
TOGUBAP 2013/117 No’lu Araştırma Projesi
References
- Agrios GN (2005) Plant Pathology. Elvesier Academic Press, London.
- Bayan Y (2011) Investigation of phenollics in resistant and susceptible tomatoes to bacterial canker disease (Clavibacter michiganensis subsp. michaganensis). MSc Thesis, Gaziosmanpasa University, Tokat.
- Çalış Ö, Topkaya Ş (2011) Genetic analysis of resistance to early blight disease in tomato. African Journal of Biotechnology 10(79):18071-18077.
- Çalış Ö, Saygı S, Çelik D, Bayan Y (2013) Resistance to bacterial canker and wilting disease of tomato and reverse genetics. Akdeniz University Journal of the Faculty of Agriculture 26(1): 5-10.
- Çalış Ö, Karabulut D, Karakaş H, Özdemir F (2015) Characterization of resistance in bacterial cancer and wilt disease resistant tomato genotypes. In: 2. Central Anatolia Region Agriculture and Food Congress, Nevşehir, Turkey, pp. 558-558.
- FAO (2021) Tomato production. http://www.faostat.fao.org. Accessed 24 February, 2021.
- Fermentas (2014) Genomic DNA purification kit K0512.www.fermentas.com. Accessed 23 January, 2014.
- Grant M, Lamb C (2006) Systemic immunity. Current Opinion in Plant Biology 9: 414-420.
- Lahaye T (2004) Illuminating the molecular basis of gene-for-gene resistance; Arabidopsis thaliana RRS1-R and its interaction with Ralstonia solanacearum popP2. Trends in Plant Science 9: 1-4.
- Meyers BC, Kaushik S, Nandety RS (2005) Evolving disease resistance genes. Current Opinion in Plant Biology 8: 129-134.
- Nash AF, Gardner RG (1988) Tomato early blight resistance in a breeding line derived from Lycopersicon hirsutum P. I. 126445. Plant Disease 72: 206-209.
- Oxoid (2013) Plate count agar tryptone glucose yeast agar protocol. http://www.oxoid.com/UK/blue/proddetail/proddetail.asp?pr=CM0325&cat=&sec=1. Accessed 18 November, 2013.
- Powell W, Machray GC, Provan J (1996) Polymorphism revealed by simple sequence repeats. Trends in Plant Science 1(7): 1360-1385.
- Rathjen JP, Moffett P (2003) Early signal transduction events in specific plant disease relocation. Nature Genetics 37: 777-782.
- Sambrook J, Fritsch EF, Maniatis T (1989) Molecular Cloning A Laboratory Manual. 2nd Edition, Cold Spring Harbor Laboratory Press, New York.
- Solgenomics (2013) Solanacea Genomics Network. http://solgenomics.net. Accessed 20 May, 2013.
- TÜİK (2021) Tomato production amount in Turkey. http://www.tuik.gov.tr. Accessed 24 February, 2021.
- Wiermer M, Feys BJ, Parker JE (2005) Plant immunity: the eds1 regulatory node. Current Opinion in Plant Biology 8: 383-389.
Details
| Primary Language | English |
|---|---|
| Subjects | Agricultural Engineering |
| Journal Section | Makaleler |
| Authors | |
| Project Number | TOGUBAP 2013/117 No’lu Araştırma Projesi |
| Publication Date | December 2, 2022 |
| Submission Date | April 11, 2022 |
| Published in Issue | Year 2022 Volume: 35 Issue: 3 |
Cite
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