Effects of salt stress on germination, seedling growth, and ion content of sweet sorghum

Year 2024, Volume: 37 Issue: 2, 91 - 98, 02.08.2024

Birgul Guden , Ousseini Kiemde , Merve Çelebi Akşahin , Bülent Uzun

https://doi.org/10.29136/mediterranean.1473692

Abstract

Salinity is one of the most common abiotic stresses in the world. It negatively affects the growth and development of sweet sorghum (Sorghum bicolor L. Moench). It significantly reduces germination and seedling growth parameters. The present study was carried out to evaluate the impact of four salinity levels (0, 100, 200, and 300 mM) on the germination and seedling growth parameters of four sweet sorghum genotypes (Erdurmus, Uzun, Srg 156, and BSS 424) and on their ion content (Na, K, Ca, and Mg). The results indicate that under nonsaline conditions, the germination percentage (GP) of all genotypes was 100%, and Erdurmus was identified as the earliest germinating genotype. The BSS 424 genotype showed a significant reduction in germination index (GI), ranging from 8.33% at 100 mM to 0.89% at 300 mM, while Erdurmus and Srg 156 showed the lowest decreases, with mean values of 15.801 and 13.901, respectively. The highest root fresh weight (RFW) value was observed in the control for all the genotypes, while Erdurmus showed the lowest decrease. Moreover, the highest decrease in Mg (0.24%) and Ca (0.17%) content was observed in Uzun, and the lowest K content was identified in BSS 424 (0.5%), whereas the highest Na content was also determined in Uzun (3.12%). Considering all the results, salt stress above 200 mM significantly affected the germination and seedling growth parameters. Therefore, lower concentrations should be taken into consideration for sustainable sorghum production.

Keywords

Biomass weight, NaCl, Root length, Shoot length, Shoot weight

References

• Asaadi AM (2009) Investigation of salinity stress on seed germination of Trigonella foenum-graecum. Research Journal of Biological Sciences 4: 1152-1155.
• Ashraf M (2004) Some important physiological selection criteria for salt tolerance in plants. Flora 199: 361-376.
• Ashraf MY, Akhtar K, Hussain F, Iqbal J (2006) Screening of different accession of three potential grass species from Cholistan desert for salt tolerance. Pakistan Journal of Botany 38: 1589-1597.
• Ashrafuzzaman M, Khan MAH, Shahidullah SM (2002) Vegetative growth of maize (Zea mays) as affected by a range of salinity. Crop Research (Hisar) 24: 286-291.
• Atokple IDK, Oppong GK, Chikpah SK (2014) Evaluation of grain and sugar yields of improved sweet sorghum (Sorghum bicolor) varieties in the guinea savanna zone of Ghana. Pinnacle Agricultural Research & Management 2: 374-382.
• Bakari H, Djomdi ZF, Roger DD, Cedric D, Guillaume P, Pascal D, Philippe M, Gwendoline C (2022) Sorghum (Sorghum bicolor L. Moench) and its main parts (By-Products) as promising sustainable sources of value-added ingredients. Waste and Biomass Valorization 14: 1023-1044.
• Bashir F, Ali M, Hussain K, Majeed A, Nawaz K (2011) Morphological variations in sorghum (Sorghum bicolor L.) under different levels of Na2SO4 salinity. Botany Research International 4: 1-3.
• Bavei V, Shiran B, Arzani A (2011) Evaluation of salinity tolerance in sorghum (Sorghum bicolor L.) using ion accumulation, proline and peroxidase criteria. Plant Growth Regulation 64: 275-285.
• Bybordi A, Tabatabaei J (2009) Effect of salinity stress on germination and seedling properties in Canola Cultivars (Brassica napus L.). Notulae Botanicae Horti Agrobotanici Cluj-Napoca 37: 71-76.
• Calone R, Sanoubar R, Lambertini C, Speranza M, Vittori Antisari L, Vianello G, Barbanti L (2020) Salt tolerance and Na allocation in Sorghum bicolor under variable soil and water salinity. Plants 9: 561.
• Chele KH, Tinte MM, Piater LA, Dubery IA, Tugizimana F (2021) Soil Salinity, a Serious Environmental Issue and Plant Responses: A Metabolomics Perspective. Metabolites 11: 724.
• Corwin DL (2021) Climate change impacts on soil salinity in agricultural areas. European Journal of Soil Science 72: 842-862.
• Ekmekçi E, Apan M, Kara T (2005) Tuzluluğun bitki gelişimine etkisi. Anadolu Journal of Agricultural Sciences 20: 118-125.
• FAOSTAT (2023) Statistical database. https://www.fao.org/faostat/fr/#rankings/countries_by_commodity. Accessed 28 March, 2023.
• Flowers TJ (2004) Improving crop salt tolerance. Journal of Experimental Botany 55: 307-319.
• Gökkaya TH, Arslan M (2023) Germination and growth parameters in sorghum cultivars (Sorghum bicolor L.) effected by boron application under salinity stress. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi 26: 629-638.
• Hakim MA, Juraimi AS, Begum M, Hanafi MM, Ismail MR, Selamat A (2010) Effect of salt stress on germination and early seedling growth of rice (Oryza sativa L.). African Journal of Biotechnology 9: 1911-1918.
• Hamada AM, Al-Hakimi AMA (2001) Salicylic acid versus salinity-drought induced stress on wheat seedlings. Rostlinna Vyroba 47: 444-450.
• Hao H, Li Z, Leng C, Lu C, Luo H, Liu Y, Wu X, Liu Z, Shang L, Jing HC (2021) Sorghum breeding in the genomic era: opportunities and challenges. Theoretical and Applied Genetics 134: 1899-1924.
• Jamil M, Lee DB, Jung KY, Ashraf M, Lee SC, Rha ES (2006) Effect of salt (NaCl) stress on germination and early seedling growth of four vegetables species. Journal of Central European Agriculture 7: 273-282.
• Joardar JC, Razir S, Islam M, Kobir MH (2018) Salinity impacts on experimental fodder sorghum production. SAARC Journal of Agriculture 16: 145-155.
• Kausar A, Ashraf MY, Ali I, Niaz M, Abbass Q (2012) Evaluation of sorghum varieties/lines for salt tolerance using physiological indices as screening tool. Pakistan Journal of Botany 44: 47-52.
• Kawasaki T, Akiba T, Moritsugu M (1983) Effects of high concentrations of sodium chloride and polyethylene glycol on the growth and ion absorption in plants. Plant Soil 75: 75-85.
• Khan MA, Weber DJ (2008) Ecophysiology of high salinity tolerant plants. Springer Science and Business Media, pp. 145-156.
• Kumar P, Sharma PK (2020) Soil salinity and food security in India. Frontiers in Sustainable Food Systems 4: 533781.
• Munns R (2002) Comparative physiology of salt and water stress. Plant, Cell and Environment 25: 239-250.
• Munns R, Tester M (2008) Mechanisms of salinity tolerance. Annual Review of Plant Biology 59: 651-681.
• Netondo GW, Onyango JC, Beck E (2004a) Sorghum and salinity: Response of growth, water relations, and ion accumulation to NaCl salinity. Crop Science 44: 797-805.
• Netondo GW, Onyango JC, Beck E (2004b) Sorghum and salinity: II. gas exchange and chlorophyll fluorescence of sorghum under salt stress. Crop Science 44: 806-811.
• Nimir A, Eltyb N, Lu S, Zhou G, Ma BL, Guo W, Wang Y (2014) Exogenous hormones alleviated salinity and temperature stresses on germination and early seedling growth of sweet sorghum. Agronomy Journal 106: 2305-2315.
• Norlyn JD, Epstein E (1984) Variability in salt tolerance of four triticale lines at germination and emergence. Crop Science 24: 1090-1092.
• Okumuş O, Kahraman ND, Oğuz MÇ, Yıldız M (2023) Magnetic field treatment in barley: Improved salt tolerance in early stages of development. Selcuk Journal of Agriculture and Food Sciences, 37(3): 556-569.
• Okumuş O, Şekerci AD (2024) Effects of different salt stress and temperature applications on germination in mung bFokean (Vigna radiata (L.) R. Wilczek) genotypes. Black Sea Journal of Agriculture 7(3): 310-316.
• Özyazıcı MA, Açıkbaş S (2021) Effects of different salt concentrations on germination and seedling growth of some sweet sorghum [Sorghum bicolor var. saccharatum (L.) Mohlenbr.] cultivars. Türkiye Tarımsal Araştırmalar Dergisi 8: 133-143.
• Pankova EI, Konyushkova MV (2013) Climate and soil salinity in the deserts of Central Asia. Eurasian Soil Science 46: 721-727.
• Rahman MS, Miyake H, Taheoka Y (2001) Effect of sodium chloride salinity on seed germination and early seedling growth of rice (Oryza sativa L.). Pakistan Journal of Biological Sciences 4: 351-355.
• Rajabi Dehnavi A, Zahedi M, Ludwiczak A, Cardenas Perez S, Piernik A (2020) Effect of salinity on seed germination and seedling development of sorghum (Sorghum bicolor (L.) Moench) genotypes. Agronomy 10: 859.
• Ranjbar GH, Cheraghi SAM, Banakar MH (2008) Salt sensivity of wheat at germination stage. In: Kafi M, Khan A (Eds). Crop and Forage Production Using Saline Waters in Dry Areas. Daya Publishing: New Dehli, India, pp. 200-204.
• Ratanavathi CV, Dayakar Rao B, Seetharama N (2004) Sweet Sorghum: A new raw material for fuel alcohol. Study Report on Technological Aspects in Manufacturing Ethyl Alcohol from Cereal Grains in Maharashtra, Part II, Prepared by Department of Scientific & Industrial Research, Ministry of Science & Technology, Government of India, New Delhi and Mitcon Cinsultancy Services Limited, Pune, pp. 32- 41.
• Rehman S, Harris PJC, Bourne WF, Wilkin J (2000) The relationship between ions; vigour and salinity tolerance of Acacia seeds. Plant Soil 220: 229-233.
• Sabagh AEL, Hossain A, Islam MS, Iqbal MA, Amanet K, Mubeen M, Nasim W, Wasaya A, Llanes A, Ratnasekera D, Singhal RK, Kumari A, Meena RS, Abdelhamid M, Hasanuzzaman M, Raza MA, Özyazici G, Özyazici MA, Erman M (2021) In: Aftab T Hakeem KR (Eds). Prospective role of plant growth regulators for tolerance to abiotic stresses. Plant Growth Regulators, Switzerland, pp. 1- 38.
• Sagar A (2017) Screening of sorghum germplasms for salinity tolerance based on morpho-physiological and biochemical traits. M.Sc Thesis, Bangladesh Agricultural University, Bangladesh.
• Sagar A, Hossain MA, Uddin MN, Tajkia JE, Mia MA et al. (2023) Genotypic divergence, photosynthetic efficiency, sodium extrusion, and osmoprotectant regulation conferred salt tolerance in sorghum. Phyton-International Journal of Experimental Botany 92: 2349-2368.
• Scott SJ, Jones RA, Williams WA (1984) Review of data analysis methods for seed germination. Crop Science 24: 1192-1199.
• Shakeri E, Emam Y (2017) Selectable traits in sorghum genotypes for tolerance to salinity stress. Journal of Agricultural Science and Technology 19: 1319-1332.
• Shakeri E, Emam Y, Pessarakli M, Tabatabaei SA (2020) Biochemical traits associated with growing sorghum genotypes with saline water in the field. Journal of Plant Nutrition 43: 1-18.
• Steduto P, Katerji N, Puertos-Molina H, Unlu M, Mastrorilli M, Rana G (1997) Water-use efficiency of sweet sorghum under water stress conditions Gas-exchange investigations at leaf and canopy scales. Field Crops Research 54: 221-234.
• Tigabu E, Andargie M, Tesfaye K (2012) Response of sorghum (Sorghum bicolor (L.) Moench) genotypes to NaCl levels at early growth stages. African Journal of Agricultural Research 7: 5711-5718.
• U.S. EPA (2007) Method 3051A (SW-846): Microwave Assisted Acid Digestion of Sediments, Sludges, and Oils. Revision 1. Washington, DC.
• Wang W, Vinocur B, Altman A (2003) Plant responses to drought, salinity and extreme temperatures: towards genetic engineering for stress tolerance. Planta 218: 1-14.
• Wang YR, Yu L, Nan ZB, Liu YL (2004) Vigor tests used to rank seed lot quality and predict field emergence in four forage species. Crop Sciences 44: 535-541.
• Wichelns D, Qadir M (2015) Achieving sustainable irrigation requires effective management of salts, soil salinity, and shallow groundwater. Agricultural Water Management 157: 31-38.
• Xu G, Zhang Y, Sun J, Shao H (2016) Negative interactive effects between biochar and phosphorus fertilization on phosphorus availability and plant yield in saline sodic soil. Science of The Total Environment 568: 910-915.
• Yan K, Xu H, Cao W, Chen X (2015) Salt priming improved salt tolerance in sweet sorghum by enhancing osmotic resistance and reducing root Na+ uptake. Acta Physiologiae Plantarum 37: 1-10.
• Yang Z, Zheng H, Wei X, Song J, Wang B, Sui N (2018) Transcriptome analysis of sweet Sorghum inbred lines differing in salt tolerance provides novel insights into salt exclusion by roots. Plant Soil 430: 423-439.
• Zhu G, An L, Jiao X, Chen X, Zhou G, McLaughlin N (2019) Effects of gibberellic acid on water uptake and germination of sweet sorghum seeds under salinity stress. Chilean Journal of Agricultural Research 79: 415-424.