Overexpression of the tomato pathogenesis-related gene SlPR-1.9 confers increased tolerance to salt stress in Arabidopsis thaliana

Year 2024, Volume: 37 Issue: 3, 147 - 154, 06.12.2024

Kubra Yildiz , M. Aydin Akbudak

https://doi.org/10.29136/mediterranean.1556782

Abstract

Pathogenesis-related (PR) proteins are essential components of plant defense mechanisms, responding to both biotic and abiotic stresses. Among these, PR-1 proteins feature a CAP (Cysteine-rich secretory proteins, Antigen 5, and Pathogenesis-related 1) domain, which is crucial for immune responses and pathogen defense due to its ability to stabilize protein structures and interact with various molecules. This study investigated the role of the tomato PR-1 gene SlPR-1.9 in enhancing salt tolerance in Arabidopsis thaliana. The gene’s coding sequence was cloned and transferred into Arabidopsis to create SlPR-1.9 overexpression lines. These transgenic lines, alongside wild-type plants, were exposed to salt stress (150 mM NaCl) to assess their tolerance. Morphological analysis revealed that the transgenic lines demonstrated greater resilience to salt stress compared to wild-type plants, with less severe leaf curling and color changes. Additionally, lower proline accumulation, a stress marker, in the transgenic lines indicated an enhanced adaptive response. Bioinformatics analysis of the protein encoded by SlPR-1.9, A0A3Q7HSC4, suggested a strong interaction with galactolipase. Expression analysis showed that SlPR-1.9 was mainly expressed in roots and during early fruit development, suggesting a significant role in root physiology and stress response. These findings indicate that overexpression of SlPR-1.9 can improve plant tolerance to salt stress, offering potential applications for enhancing crop resilience to environmental challenges.

Keywords

SlPR-1.9, Salt stress, Arabidopsis thaliana, Pathogenesis-related proteins, Galactolipase

Supporting Institution

Akdeniz University

Thanks

This study was supported by Akdeniz University Scientific Research Projects Coordination Unit grant no. FYL-2022-5900. MAA is thankful for the financial support provided by the Fulbright Visiting Scholar Grant from the Turkish Fulbright Commission.

Overexpression of the tomato pathogenesis-related gene SlPR-1.9 confers increased tolerance to salt stress in Arabidopsis thaliana

Abstract

Keywords

SlPR-1.9, Salt stress, Arabidopsis thaliana, Pathogenesis-related proteins, Galactolipase

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