Plant tissue culture of Nicotiana tabacum cv. TAPM 26 and its minimum inhibition against herbicide-Dalapon

Yıl 2021, , 35 - 42, 30.04.2021

Karwan Taalat , Muhammad Arshad Javed , Fahrul Zaman Huyop , Yilmaz Kaya

https://doi.org/10.51354/mjen.839516

Cited By: 4

Öz

Current study is to establish a basic plant tissue culture of Nicotiana tabacum TAPM 26 and test the plant tissue on resistancies against 2,2 DCP an active ingredient in herbicide-Dalapon. During micropropagation, the surface sterilization method was ascertained on seeds of tobacco. HgCl2 was used to disinfect tobacco seeds at different concentrations (0.05 gL-1, 0.2 gL-1, 0.5 gL-1 and 1.0 gL-1) within three minutes. About 70% seeds were survived when exposed to 0.05 gL-1 of HgCl2, whereas, no seeds were germinated when sterilized at concentrations above 0.05 gL-1 of HgCl2. To optimize an efficient protocol of shoots and callus formation during in vitro regeneration, explant types and plant growth were studied. Growth regulators NAA (0.1 mgL-1, 0.2 mgL-1, 0.5 mgL-1, 1.0 mgL-1 and 2.0 mgL-1) and BAP (1.0 mgL-1, 2.0 mgL-1, 3.0 mgL-1 and 4.0 mgL-1) were used. The explants types were one month old leaves and two weeks old cotyledons. The maximum numbers of shoots per explants were obtained from cotyledon with combination 0.1 mgL-1 NAA and 1.0 mgL-1 BAP. The highest callus fresh weight was achieved when NAA 0.5 mgL-1 with BAP 1.0 mgL-1 after four weeks. Thus, the highest number of shoots produced per explants from leaves culture on the MS media containing 0.2 mgL-1 NAA and 4.0 mgL-1 BAP. The best callus fresh weight was obtained with combination of 1.0 mgL-1 NAA and 1.0 mgL-1 BAP by using leaves explant. Finally, Dalapon (5 gL-1, 10 gL-1, 15 gL-1 and 20 gL-1) were applied onto leaves and cotyledon cultures of N. tabacum to check on the minimum concentration of inhibition. The minimum concentration of inhibition of leaves and cotyledon cultures of N. tabacum was at 5 gL-1 of 2,2DCP but not at 10 gL-1, 15 gL-1 and 20 gL-1. This investigation will shed alight for future studies on transgenic tobacco resistant against Dalapon

Anahtar Kelimeler

Seeds sterilization, NAA, BAP, callus formation, Shoot formation, Dalapon

Kaynakça

• 1. Bidabadi S S & Jain S M (2020). Cellular, Molecular, and Physiological Aspects of In Vitro Plant Regeneration. Plants 9: 702
• 2. Mohammed S (2020). Effects and quantity ranges of some auxins on embryogenic callusinduction from upland rice cultivars: An overview, International Journal of Life Sciencesand Biotechnology 3(2):197-204
• 3. Afroz M, Akter S, Ahmed A, Rouf R, Shilpi JA, Tiralongo E, Sarker SD, Göransson U % Uddin SJ (2020). Ethnobotany and Antimicrobial Peptides From Plants of the Solanaceae Family: An Update and Future Prospects. Frontiers in Pharmacology 11:565
• 4. Nielsen M T (1987). Tobacco. Outlook on Agriculture 16(2):77–81
• 5. Charlton A. (2004). Medicinal uses of tobacco in history. Journal of the Royal Society of Medicine 97(6): 292–296
• 6. Azeem S A, Ullah I, Ali M, Khan A, Bakht J S A (2010). Effects of different sterilents on seeds and Callusing frequency as effected by hormones in Nicotiana tabacum L. Biofrontiers 1: 62-67
• 7. Gebhardt C (2016). The historical role of species from the Solanaceae plant family in genetic research. Theoretical and Applied Genetics 129(12):2281-2294
• 8. Kumar V & Maherchandani N (1988). Differentiation in callus cultures of a tobacco (Nicotiana tabacum cv. White Burley) variant: some biochemical aspects. Plant Cell, Tissue and Organ Culture 14: 177-185
• 9. Kaya Y, Yilmaz S, Marakli S, Gozukirmizi N & Huyop F (2013) Transformation of Nicotiana tabacum with DehE Gene. Journal of Food Agriculture and Environment 11(3-4):777-780
• 10. Kaya Y, Yilmaz S, Gozukirmizi N & Huyop F (2013). Evaluation of transgenic Nicotiana tabacum with dehE gene using transposon based IRAP . American Journal of Plant Sciences 4: 41-44 11. Ganapathi T, Suprasanna P, Rao P S & Bapat V (2004). Tobacco (Nicotiana tabacum L.)-A model system for tissue culture interventions and genetic engineering. Indian Journal of Biotechnology 3:171-184
• 12. Ma J K, Drossard J, Lewis D, Altmann F, Boyle J, Christou P, & Cole, T. et al (2015) Regulatory approval and a first-in-human phase I clinical trial of a monoclonal antibody produced in transgenic tobacco plants. Plant Biotechnology Journal 13(8):1106–1120.
• 13. Habibi P, Daniell H, Soccol C R, Grossi-de-Sa M F (2019). The potential of plant systems to break the HIV-TB link. Plant Biotechnology Journal 17(10):1868-1891.
• 14. Phan H T, Pohl J, Floss D M, Rabenstein F, Veits J, Le B T, Chu H H, Hause G, Mettenleiter T & Conrad U(2013). ELPylated haemagglutinins produced in tobacco induce potentially neutralizing antibodies against H5N1 virus in mice. Plant Biotechnology Journal 11:582–593
• 15. Zhong X, Qi G, Yang J, Xing G, Liu J, & Yang X (2014). High-efficiency expression of a receptor-binding domain of SARS-CoV spike protein in tobacco chloroplasts. Sheng Wu Gong Cheng Xue Bao 30(6):920-930
• 16. Demurtas O C, Massa S, Illiano E, De Martinis D, Chan P K, Di Bonito P & Franconi R (2016). Antigen production in plant to tackle infectious diseases flare up: The case of SARS. Frontiers in Plant Science 7: 54
• 17. Yan M M, Xu C, Kim C H, Um Y C, Bah A A, & Guo D P (2009). Effects of explant type, culture media and growth regulators on callus induction and plant regeneration of Chinese jiaotou (Allium chinense). Scientia Horticulturae 123(1):124–128.
• 18. Gourguillon L, Rustenholz C, Lobstein A, & Gondet L (2018). Callus induction and establishment of cell suspension cultures of the halophyte Armeria maritima (Mill.) Willd. Scientia Horticulturae 233:407–411
• 19. Mehmood F, Abdullah U Z, Shahzadi I, Ahmed I, Waheed M T, Poczai P & Mirza B (2020). Plastid genomics of Nicotiana (Solanaceae): insights into molecular evolution, positive selection and the origin of the maternal genome of Aztec tobacco (Nicotiana rustica). Peer Journal 8:e9552
• 20. Olmstead R G, Bohs L, Migid H A & Santiago-valentin E (2008). Molecular phylogeny of the Solanaceae. Molecular Phylogenetics and Evolution 57:1159–1181.
• 21. Dupin J, Matzke N J, Särkinen T, Knapp S, Olmstead R G, Bohs L, & Smith S D (2016). Bayesian estimation of the global biogeographical history of the Solanaceae. Journal of Biogeography, 44(4), 887–899
• 22. Suzuki K, Ichiro Y & Nobukazu T (2002). Tobacco plants were transformed by Agrobacterium rhizogenes infection during their evolution. The Plant journal 32: 775-787.
• 23. Kaya Y, Marakli S, Gozukirmizi N, Mohamed E, Javed M A & Huyop F (2013) Herbicide Tolerance Genes Derived from Bacteria. The Journal of Animal and Plant Sciences 23(1):85-91
• 24. Kanwal M, Joyia F A, Mustafa G, Zia M A, Rana I A, & Khan M S (2017). Direct in vitro regeneration of Nicotiana plumbaginifolia L. and the potential for genetic transformation. International Journal of Horticulture 7(6):40-46
• 25. Liu H, Kotova T I & Timko M P (2019). Increased leaf nicotine content by targeting transcription factor gene expression in commercial flue-cured tobacco (Nicotiana tabacum L.). Genes(Basel) 10(11):930.
• 26. Sun H, Sun X, Wang H, & Ma X (2020). Advances in salt tolerance molecular mechanism in tobacco plants. Hereditas 157(5):1-6
• 27. Makenzi N G, Mbinda W M, Okoth R O & Ngugi MP (2018). In vitro plant regeneration of sweetpotato through direct shoot organogenesis. Journal of Plant Biochemistry and Physiology 6(1):207
• 28. Latifian E, Arvas Y E & Kaya Y (2018). Tagetes minuta bitkisinin mikro üretimi üzerinefarklı BAP ve IAA konsantrasyonlarının etkileri, International Journal of Life Sciences and Biotechnology 1(2): 96-104.
• 29. Kaya Y & Karakutuk S (2018) Effects of different growth regulators on regeneration of Turkish upland rice. Anadolu Tarım Bilimleri Dergisi 33(3): 226-231
• 30. Aboshama H M & Atwa MM (2019). In vitro Evaluation of Somaclonal Variation of Two Potato Cultivars Santana and Spunta for Resistance against Bacterial Blackleg Pectobacterium atrosepticum. Journal of Plant Biochemistry & Physiology 7(3): 243-253
• 31. Mangena P (2020). Benzyl adenine in plant tissue culture- succinct analysis of the overall influence in soybean [Glycine max (L.) Merrill.] seed and shoot culture establishment. Journal of Biotech Research 11:23-34
• 32. Hussein N B M, Huyop F & Kaya Y (2020). An easy and reliable method for establishment and maintenance of tissue cultures of Nicotiana tabacum cv. TAPM 26. International Journal of Science Letters 2(2): 62-71
• 33. Kutty P C, Parveez G K A & Huyop F (2010). An easy method for Agrobacterium tumefaciens-mediated gene transfer to Nicotiana tabacum cv. TAPM26. Journal of Biological Sciences 10(6): 480-489
• 34. Kutty P C, Parveez G K A & Huyop F (2011). Agrobacterium tumefaciens-infection strategies for greater transgenic recovery in Nicotiana tabacum cv. TAPM26, International Journal of Agricultural Research 6(2):119-113
• 35. Geethalakshmi S, Balakrishnan H & Natarajan S (2016). Optimization of media formulations for callus induction, shoot regeneration and root induction in Nicotiana benthamiana. Journal of Plant Science & Research 3(1) 150-154
• 36. Eibl R (2018) Plant cell culture technology in the cosmetics and food industries : current state and future trends. Applied Microbiology and Biotechnology 102(20):8661– 8675
• 37. Al-remi F, Arvas Y E, Durmuş M, Kaya Y (2018).Tomato Plant and Its In Vitro Micropropagation. Journal of Engineering Technology and Applied Sciences 3(1), 57-73
• 38. Arvas Y E, Aksoy H M, Kaya Y (2018). Patates bitkisinde biyoteknolojik çalışmalar, International Journal of Life Sciences and Biotechnology 1(1): 37-47
• 39. Ali G, Fazal H, Zahir A, Muhammad T & Muhammad A K (2007). Callus Induction and in vitro Complete Plant Regeneration of Different Cultivars of Tobacco (Nicotiana tabacum L.) on Media of Different Hormonal Concentrations. Biotechnology 6:561-566
• 40. Vissenberg K, Feijo J A, Weisenseel M H & Verbelen J P (2001). Ion fluxes, auxin and the induction of elongation growth in Nicotiana tabacum cells. Journal of Experimental Botany 52:2161-2167.
• 41. Eklof S, Astot C, Sitbon F, Moritz T, Olsson O, & Sandberg G (2000). Transgenic tobacco plants co-expressing Agrobacterium iaa and ipt genes have wild-type hormone levels but display both auxin- and cytokinin-overproducing phenotypes. The Plant Journal 23(2):279–284
• 42. Skoog F & Miller C O (1957).Chemical regulation of growth and organ formation in plant tissues cultured in vitro.Symposia of the Society for Experimental Biology 54:118–130
• 43. Allawzi M, & Kandah M I (2008). Parametric study of biodiesel production from used soybean oil. European Journal of Lipid Science and Technology 110:760-767
• 44. Duke S O, Scheffler B E, Boyette C D & Dayan F E (2015). Biotechnology in weed control. In: Kirk-Othmer encyclopedia of chemical technology. Wiley, New York, pp 1–25
• 45. Beckie H J, Ashworth MB & Flower KC (2019). Herbicide resistance management: recent developments and trends. Plan Theory 8(6):161.
• 46. Dikshith T S & Diwan P V (2003). Industrial Guide to Chemi and Drug Safety.John Wiley&Sons,Inc.
• 47. Hawker D W, Cumming J L, Watkinson A, & Bartkow M E (2011). The occurrence of the herbicide dalapon (2,2-dichloropropionate) in potable water as a disinfection by-product. Journal of Environmental Monitoring 13(2):252–256
• 48. Akcay Kazim & Yilmaz Kaya (2019) Isolation, characterization and molecular identification of a halotolerant Bacillus megaterium CTBmeg1 able to grow on halogenated compounds, Biotechnology & Biotechnological Equipment, 33:1, 945-953, DOI: 10.1080/13102818.2019.1631717
• 49. Arvas, Y. E., & Kaya, Y. (2019). Genetiği değiştirilmiş bitkilerin biyolojik çeşitliliğe potansiyel etkileri. Yüzüncü Yıl Üniversitesi Tarım Bilimleri Dergisi, 29(1), 168-177
• 50. Heaton, A.C.P., Rugh, C.L., Wang, NJ. et al. Physiological Responses of Transgenic merA-TOBACCO (Nicotiana tabacum) to Foliar and Root Mercury Exposure. Water Air Soil Pollut 161, 137–155 (2005). https://doi.org/10.1007/s11270-005-7111-4
• 51. Mehmood F (2016). Effect of different parameters on transformation and regeneration of nodal and leaf explants of Nicotiana tabacum cv. Petiat Havana. Master thesis. Quaid-i-Azam University, Islamabad, Pakistan
• 52. Smulders M J M, van de Ven E T W M, Croes A F & Wullems G J (1990). Metabolism of 1-naphthaleneacetic acid in explants of tobacco: Evidence for release of free hormone from conjugates. Journal of Plant Growth Regulation 9(1-4):27-34
• 53. Dhaliwal H S (1998). Competence and determination during Shoot and Root Organogenesis in vitro. Master of Science Thesis, University of Calgary, Canada
• 54. Attfield E M & Evans P K (1991). Developmental pattern of root and shoot organogenesis in cultured leaf explants of Nicotiana tabacum. Journal of Experimental Botany 42:51-57
• 55. Stolarz A, Macewicz J & Lörz H (1991). Direct somatic embryogenesis and plant regeneration from leaf explants of Nicotiana tabacum L. Journal of Plant Physiology 137(3):347–357
• 56. Vajjiram C, Vanitha A & Kalimuthu K (2017). Ftir and gc-ms analysis of bioactive compounds in ethanol extract of tobacco callus (Nicotiana tabacum). World Journal of Pharmaceutical Research 6(14):530-545.
• 57. Gelvin S B (2003). Agrobacterium-Mediated Plant Transformation: the Biology behind the “Gene-Jockeying” Tool. Microbiology and molecular biology reviews 67(1): 16-37