Determination of Amino Acid Composition of Cow’s Milk by Liquid Chromatography Using Precolumn Derivatization

Yıl 2017, Cilt: 5 Sayı: 3, 25 - 34, 01.12.2017

A. MAZHİTOVA A. KULMYRZAEV

Öz

The application of the procedure of pre-column derivatization with diethyl
ethoxymethylenemalonate in the determination of the amino acid content of milk by reversedphase
chromatography with diode-matrix detection was investigated. The influence of the pH on
the response factor was established. The conditions of modification of amino acids during
sample preparation procedure were optimized. A complete separation of 18 amino acids was
achieved. The most abundant amino acid in cow's milk was glutamic acid, the concentration of
which twice that of leucine and lysine, and three times greater than aspartic acid

Anahtar Kelimeler

Аминокислота, белки, молоко, хроматография

Kaynakça

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