Effect of Artificial Light Sources on DNA Damage in Human Mononuclear Lymphocyte Cells Under In Vitro Conditions

Öz

Aim: This study aimed to evaluate the effects of commonly used artificial light sources on DNA damage and oxidative stress parameters in human mononuclear lymphocyte cells. The potential effects of different lighting sources on cellular DNA integrity and oxidative stress levels were comprehensively evaluated. Methods: Isolated human mononuclear lymphocyte cells were exposed to artificial light sources at varying intensities and durations. DNA damage was analyzed using the alkaline single-cell gel electrophoresis (comet assay) method. The oxidative status of the cells was assessed using Erel’s method. Results: The highest DNA damage was observed with white and yellow compact fluorescent lamps, whereas the least damage occurred with yellow incandescent light sources. Additionally, 100-watt fluorescent lamps caused the most DNA damage, while the least damage was detected with 20-watt lamps. Oxidative stress index levels significantly increased with prolonged exposure. Conclusion: Our findings indicate that artificial light sources can induce DNA damage by increasing both direct and oxidative stress. Different light types and exposure durations significantly affect cellular genotoxicity and oxidative stress levels. These results provide an important insight into the potential biological risks associated with artificial lighting.

Anahtar Kelimeler

• Artificial Light Sources
• Comet Assay
• DNA Damage
• Oxidative Stress

In Vitro koşullarda farklı güç ve cinsteki aydınlatma kaynaklarının mononükleer hücre DNA hasarı üzerine etkisinin araştırılması

Öz

Amaç: Bu çalışmanın amacı, günlük yapay ışık kaynaklarının insan mononükleer lenfosit hücrelerinde DNA hasarı ve oksidatif stres parametreleri üzerindeki etkilerini araştırmaktır. Farklı aydınlatma kaynaklarının hücresel DNA bütünlüğü ve oksidatif stres düzeylerine yönelik potansiyel DNA hasarı etkileri kapsamlı bir şekilde değerlendirmektir. Yöntem: İzole edilmiş insan mononükleer lenfosit hücreleri çeşitli yoğunluklarda yapay ışık kaynaklarına maruz bırakılmıştır. DNA hasarının analizi için alkalin tek hücreli jel elektroforezi (comet assay) yöntemi kullanılmıştır. Hücrelerin oksidatif durumları ise Erel yöntemiyle değerlendirilmiştir. Bulgular: En fazla DNA hasarının, beyaz ve sarı kompakt floresan lambalarda saptanmıştır. En az DNA hasarının ise sarı akkor ışık kaynaklarında meydana geldiği belirlenmiştir. Ayrıca, 100 watt'lık floresan ışık kaynaklarının en fazla DNA hasarına neden olduğu saptanmıştır. En az hasar ise 20 watt'lık lambalarda gözlemlenmiştir. Işığa maruz kalma süresi arttıkça oksidatif stres indeksinin anlamlı derecede yükseldiği tespit edilmiştir. Sonuç: Çalışmamız, yapay ışık kaynaklarının hem direk hem de oksidatif stres düzeylerini artırarak DNA hasarına neden olabileceğini ortaya koymuştur. Bulgularımız, farklı yapay ışık kaynaklarının ve maruziyet sürelerinin hücresel genotoksisite ve oksidatif stres üzerinde belirgin etkiler yarattığını göstermektedir. Bu sonuçlar, yapay aydınlatmanın potansiyel biyolojik risklerini göstermektedir.

Anahtar Kelimeler

• Yapay Işık Kaynakları
• Comet Assay
• DNA Hasarı
• Oksidatif Stres

Kaynakça

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