Evaluation of the cytotoxic effects of monomer releases of two types of binding agents on pulp fibroblast cells.

Abstract

Abstract Aim: The study was performed for investigating the cytotoxic effects of Single Bond and Adper Prompt-L-Pop dentin bonding agents used for restorations of primary and permanent teeth, on the human pulp fibroblasts. Method: The primary culture of human pulp fibroblast was prepared and the third passage of the fibroblasts were used. The materials used in the study were put into the glass carriers designed for inhibiting the materials' direct contact with the fibroblasts and one group of Single Bond and Adper Prompt-L-Pop were polymerized while the other groups were left unpolymerized. After 24-hour incubation, the cell proliferations were determined respectively by the BrdU assay. The monomer releases of the bonding agents were also determined using HPLC (High performence liquid kromatogrephy) device. The correlation between these values were evaluated. Results: In the study, the cell proliferation of the polymerized Single Bond and Adper Prompt-L-Pop groups were found significantly higher than the unpolymerized Single Bond and Adper Prompt-L-Pop groups. Conclusion: In conclusion, the bonding agents Single Bond and Adper Prompt-L-Pop when used according to the manufacturers' proposal had a minimum cytotoxic effect on the human pulp fibroblasts. However, the results of the study have indicated that degeneration in the monomer-polymer chain caused the monomer release which is cytotoxic on the human pulp. For this reason, dentists have to be careful about the application of the bonding agents for the success of the

Keywords

• Citotoxity
• Proliferation
• Pulp cells
• Monomer release

İki tip bağlayıcı ajanın monomer salınımlarının pulpa fibroblast hücreleri üzerindeki sitotoksik etkilerinin değerlendirilmesi.

Öz

Amaç: Bu çalışma, süt ve sürekli dişlerin restorasyonlarında kullanılan kompozit dolgu materyallerinin dentine tutunmasını sağlayan bağlayıcı sistemlerin sitotoksik etkilerini belirlemek amacıyla gerçekleştirilmiştir. Yöntem: Tek şişe (one-bottle) sistemler grubu içinde bulunan Single Bond ve kendinden asitlemeli (self-etching) sistemler grubu içinde bulunan Adper Prompt-L-Pop materyallerinin insan pulpası fibroblast hücreleri üzerindeki sitotoksik etkileri incelenmiştir. Her iki ajanın da bir grubu üretici firmaların önerileri doğrultusunda polimerize edilmiş, bir grubu da polimerize edilmeden bırakılmış ve bu grupların pulpa fibroblast hücreleri üzerinde meydana getirdiği değişiklikler hücre proliferasyonu açısından değerlendirilmiştir. İnsan pulpa fibroblastlarının primer hücre kültürü hazırlanmış ve çalışmada üçüncü pasajı kullanılmıştır. Materyaller özel olarak hazırlanmış cam taşıyıcılar içerisinde, hücrelerle doğrudan temas etmesi engellenerek yirmi dörtlü well- platelerdeki kuyucuklara yerleştirilmiştir. 24 saat inkübisyondan BrdU boyama yöntemi ile hücer proliferasyonları belirlenmiştir. Bağlayıcı ajanların monomer salınımları ise HPLC (Yüksek performanslı sıvı kromatografi) cihazı kullanılarak saptanmıştır. Bu iki bulgu arasındaki korelasyon değerlendirilmiştir. Bulgular: Araştırma sonucunda, Single Bond ve Adper Prompt-L-Pop materyallerinin polimerize edilmiş olan gruplarında, hücre proliferasyon oranlarının polimerize edilmemiş gruplarla karşılaştırıldığında belirgin bir şekilde yüksek olduğu saptanmıştır. Sonuç: Sonuç olarak, günümüzde kullanılan Single Bond ve Adper Prompt- L-Pop materyallerinin üretici firma önerileri doğrultusunda kullanılmasının pulpa dokusu üzerinde olumsuz etki göstermeyeceği, ancak yapılarında bulunan monomerlerin polimerlere dönüşümü sağlanamadığında, gerçekleşen monomer salınımının pulpa üzerinde yüksek oranda sitotoksisite gösterdiği belirlenmiştir. Bu nedenle, bağlayıcı ajanları uygularken gerekli özeni göstermek restorasyonun başarısı açısından büyük önem taşımaktadır.

Anahtar Kelimeler

• sitotoksite
• proliferasyon
• monomer salınımı
• pulpa hücreleri

Kaynakça

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