BİYOLOJİK OLARAK SENTEZLENMİŞ GÜMÜŞ NANOPARTİKÜLLERİN SİTOTOKSİSİTESİ VE YARA İYİLEŞTİRME KAPASİTESİ

Abstract

CYTOTOXICITY AND WOUND HEALING CAPACITY OF BIOLOGICALLY SYNTHESIZED SILVER NANOPARTICLES

Abstract

Metallic nanoparticles are interesting areas of research due to their unique properties which can be advantageous for producing smart products. Silver nanoparticles (AgNPs) are remarkably used in pharmaceutical industry because of their strong biological activities. The aim of the present study was to investigate the cytotoxicity and wound healing capacity of the biologically prepared silver nanoparticles via green synthesis route. Cytotoxicity of the biogenic AgNPs was determined by MTT (3- [4, 5- dimethyl-2-thiazolyl]-2, 5-diphenyl-2H-tetrazolium-bromide) assay against L929 fibroblast cell line. Wound healing properties of the AgNPs were evaluated using in vitro-scratch wound healing assay using 3T3 fibroblast cell line. Biosynthesized AgNPs inhibited the propagation of fibroblasts at a half maximal inhibitory concentration (IC₅₀) of 23.507 μg/mL after 24 h incubation. In vitro wound healing assay also revealed that the biogenic AgNPs stimulated the 3T3 fibroblasts' cell proliferation. It can be suggested that biologically synthesized AgNPs can be used effectively for biomedical applications such as wound dressing materials.

Keywords

• silver nanoparticle
• green synthesis
• cytotoxicity
• wound healing

Thanks

Cytotoxicity analysis was performed at AUBIBAM (Anadolu University Medicinal Plants, Drugs and Scientific Research Center), Anadolu University, Eskişehir, Türkiye.

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