REDUCED GRAPHENE OXIDE AND Tb-DO3A CONJUGATE AS LUMINESCENT CHEMOSENSOR FOR AGILE DETECTION OF HYDROXYL RADICAL

Öz

The development of chemosensors for the detection of hydroxyl radicals (HO•) is a challenging task since HO• has an exceptionally short lifetime (in vivo half-life of 1 ns). In this work, we have designed and synthesized a versatile probe, viz. Tb@rGO, for the detection of HO• amongst the biologically important ions and reactive oxygen species (ROS). Our design is based on covalent conjugation of reduced graphene oxide (rGO) with terbium (III)-1,4,7,10-tetraazacyclododecane-1,4,7-triacetic acid (Tb-DO3A). Tb@rGO is characterized by traditional spectroscopic methods including XRD, SEM, TEM, and zeta potential analysis. Furthermore, we elaborate the photophysical properties of Tb@rGO. Accordingly, our results attest that Tb@rGO has unique luminescence features, rendering it highly effective in the detection of HO•. Remarkably, Tb@rGO is highly selective to HO• among many biologically important species in 0.1 M pH 7.4 phosphate buffered saline solution. It is also noteworthy that the limit of detection (LOD) is 0.92 M for HO•. Therefore, this novel material hold promises as selective turn-off luminescent HO• probe.

Anahtar Kelimeler

• Terbium (III)
• Reduced graphene oxide
• Reactive oxygen species
• Hydroxyl radical
• Luminescent probe

HİDROKSİL RADİKALİNİN HIZLI TESPİTİ İÇİN LÜMİNESANS KEMOSENSÖR OLARAK iNDİRGENMİŞ GRAFEN OKSİT VE Tb-DO3A KONJÜGATI

Öz

Hidroksil radikallerinin (HO•) tespiti için kemosensörlerin geliştirilmesi, HO•’nun son derece kısa bir ömre sahip olması (in vivo yarılanma ömrü ~1 ns) nedeniyle zorlu bir iştir. Bu çalışmada biyolojik olarak önemli iyonlar ve reaktif oksijen türleri (ROS) arasında HO•’nun tespiti için kullanılabilecek çok yönlü bir prob Tb@rGO tasarladık ve sentezledik. Tasarımımız indirgenmiş grafen oksidin (rGO) terbiyum (III)-1,4,7,10-tetraazasiklododekan-1,4,7-triasetik asit (Tb-DO3A) ile kovalent konjugasyonuna dayanmaktadır. Tb@rGO, XRD, SEM, TEM ve zeta potansiyel analizini içeren geleneksel spektroskopik yöntemlerle karakterize edilmiştir. Ayrıca Tb@rGO’nun fotofiziksel özelliklerini de detaylandırdık. Buna göre, sonuçlarımız Tb@rGO’nun benzersiz lüminesans özelliklere sahip olduğunu ve bu özelliğin onu HO• tespitinde son derece etkili kıldığını doğrulamaktadır. Dikkat çekici bir şekilde Tb@rGO fosfat tamponlu salin (0,1 M PBS, pH 7,4) çözeltisinde biyolojik açıdan önemli birçok tür arasında HO•’ya karşı oldukça seçicidir. HO• için tespit sınırının (LOD) 0,92 M olması da dikkat çekicidir. Bu nedenle, bu yeni malzeme, seçici lüminesans sönümlemeli HO• probu olarak umut vaat etmektedir.

Anahtar Kelimeler

• Terbiyum (III)
• İndirgenmiş grafen oksit
• Reaktif oksijen türleri
• Hidroksil radikali
• Lüminesans prob

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