Biochemical and Histopathological Evaluation of the Protective Efficacy of Thymoquinone in Experimentally Ischemia Reperfusion Induced Rat Ovaries

Abstract

Aim: In our study, oxidant-antioxidant and inflammation markers of ovarian tissue were evaluated in order to determine whether thymoquinone has a protective effect against experimentally induced ischemia-reperfusion in rat ovaries. In our study, oxidant-antioxidant and inflammation markers in ovarian tissue were evaluated in order to determine whether thymoquinone has a protective effect against damage in rat ovaries in which ischemia-reperfusion was created. Material and Method: The rats, which randomly divided into 5 groups and were experimentally induced ischemia-reperfusion in their ovaries, were administered timokinon at the determined doses. The markers such as TAS, TOS and MDA from ovarian tissues were determined as spectrophotometrically obtained by experimental procedure. Tissues examined histopathologically under light microscopy were evaluated for immunopositivity after immunohistochemical staining with NF-Kβ1 antibodies. Results: While MDA, TOS, OSI levels in tissue were significantly higher in ischemia/reperfusion groups, and TQ, MDA, TOS, OSI levels were significantly lower rather than the control group (p<0.05). TAS levels in tissue were significantly lower in the I/R group but after treatment they increased significantly (p<0.05). NFKβ1 immunopositivity could not be detected in Sham and experimental groups (p> 0.05). Conclusion: It was concluded that thymoquinone protected the tissues from ischemic damage by causing significant increase in TAS levels that were decreased by I/R injury and significant decrease in elevated levels of MDA and TOS that were increased by I/R injury.

Keywords

• Ischemia Reperfusion
• Oxidative stress
• Thymoquinone

References

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