Antiproliferative and apoptotic effects of Onobrychis albiflora extract on HCT- 116 cells

Year 2018, Volume: 35 Issue: 4, 101 - 109, 18.11.2019

Muhammet Volkan Bülbül , Hanefi Özbek Ozlem Bahadır Acıkara , Serkan Ozbılgın , Burcın Ergene Oz Ekın Kurtul İlknur Keskın

Abstract

In this study, the cytotoxic effects of the extracts of methanol: water (80:20)
prepared from the above-ground parts of the varieties of Onobrychis albiflora
Hub.-Mor., Onobrychis argyrea Boiss. subsp. argyrea, Onobrychis galegifolia
Boiss. and Onobrychis tournefortii (Willd.) Desv. species to HCT-116 cells
were investigated. With cytotoxicity analysis that with these species inhibitor
concentrations (IC50) which resulted in a 50% reduction in the proliferation of
HCT-116 cells were identified. In continuation of the study; the antiproliferative
and apoptotic effects of Onobrychis albiflora extract on HCT-116 cells were
evaluated by Caspase 3, Annexin V / PI Apoptosis / Necrosis analysis, Apopxin
Green and 7-AAD Apoptosis / Necrosis analysis. The antiproliferative, apop-
totic and necrotic effects of Onobrychis albiflora extract on HCT-116 cells also
were determined. 

Keywords

HCT-116, FACS, HEK-293, NECROSİS, APOPTOSİS, COLON CANCER

Project Number

Bap 2016/18

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