Can Apigenin Be an Effective Therapeutic Agent Against Experimental Renal Ischemia-Reperfusion Injury?

Abstract

Aim: This study aims to reveal the effects of two doses of apigenin (API) against renal ischemia-reperfusion injury (R I/R). Material and Methods: For this purpose, 5 and 10 mg/kg doses of API were preferred in our study, and the groups were designed as sham, R I/R, 5 mg/kg API, and 10 mg/kg API groups for the implementation of the experimental protocol. In the R I/R model, 1-hour ischemia and 24-hour reperfusion periods were preferred. Oxidative and inflammatory markers were measured biochemically in samples taken at the end of the experiment. Results: Biochemical results showed that oxidative and inflammatory markers increased significantly in the R I/R group, but antioxidant activities decreased significantly. In the 5 and 10 mg/kg API groups, R I/R damage was alleviated considerably, with these markers approaching the sham group values. Conclusion: As a result, the study's results determined that two different doses of API were effective against R I/R-induced kidney damage.

Keywords

• Apigenin
• kidney
• ischemia/reperfusion.

Apigenin Deneysel Renal İskemi-Reperfüzyon Hasarına Karşı Etkili Bir Terapötik Ajan Olabilir mi?

Abstract

Amaç: Bu çalışma, iki doz apigenin (API) kullanımının renal iskemi-reperfüzyon hasarına (R I/R) karşı etkilerini ortaya koymayı amaçlamaktadır. Gereç ve Yöntem: Bu amaçla çalışmamızda API'nin 5 ve 10 mg/kg dozları tercih edilmiş ve deneysel protokolün uygulanması için gruplar sham, R I/R, 5 mg/kg API ve 10 mg/kg API grupları olarak tasarlanmıştır. R I/R modelinde 1 saatlik iskemi ve 24 saatlik reperfüzyon periyotları tercih edilmiştir. Deney sonunda alınan örneklerde oksidatif ve inflamatuvar belirteçler biyokimyasal olarak ölçüldü. Bulgular: Biyokimyasal sonuçlar oksidatif ve inflamatuar belirteçlerin R I/R grubunda anlamlı olarak arttığını, ancak antioksidan aktivitelerin anlamlı olarak azaldığını gösterdi. 5 ve 10 mg/kg API gruplarında, R I/R hasarı önemli ölçüde hafifledi ve bu belirteçler sham grubu değerlerine yaklaştı. Sonuç: Sonuç olarak, çalışmanın sonuçları iki farklı API dozunun R I/R ile indüklenen böbrek hasarına karşı etkili olduğunu göstermiştir.

Keywords

• Apigenin
• böbrek
• iskemi/reperfüzyon

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