Protective Effects of Apocynin On Cecal Ligation and Puncture-Induced Lung and Renal Injuries

Abstract

Aim: The possible beneficial properties of Apocynin against cecal ligation and puncture (CLP) induced lung and renal injuries were investigated in rats. Materials and Methods: 32 Wistar Albino male rats were randomized as: group I (sham), group II (CLP), group III (CLP+Apocynin 20 mg/kg), and group IV (CLP+Apocynin 50 mg/kg). CLP process was carried out under anesthesia conditions. Apocynin was administered intraperitoneally prior to the CLP model. The lung and renal tissues were excised following the experiment. Biochemical analyzes were done. Results and Conclusion: TNF-α, OSI, IL-1β, TOS, MDA levels and MPO activity elevated but SOD and TAS values declined in CLP group compared to sham group. On the other hand, SOD and TAS levels increased while MPO activity, TNF-α, IL-1β, TOS, OSI and MDA levels declined due to Apocynin treatments. As a conclusion, Apocynin is an effective agent against CLP-induced lung and renal injuries.

Keywords

• Apocynin
• Cecal Ligation and Puncture
• Lung
• Renal
• Rat

Apokininin Çekal Ligasyon ve Ponksiyon ile İndüklenen Akciğer ve Böbrek Hasarına Karşı Koruyucu Etkileri

Abstract

Amaç: Sıçanlarda Apokinin’in, çekal ligasyon ve ponksiyona (CLP) bağlı akciğer ve böbrek hasarına karşı olası yararlı özellikleri araştırıldı.
Gereç ve Yöntem: 32 Wistar Albino erkek sıçan randomize olarak grup I (sham), grup II (CLP), grup III (CLP+Apokinin 20 mg/kg) ve grup IV (CLP+Apokinin 50 mg/kg)
olarak düzenlendi. CLP işlemi anestezi koşulları altında gerçekleştirildi. Apokinin, CLP modelinden hemen önce intraperitonal olarak uygulandı. Akciğer ve böbrek dokuları
deneyin ardından eksize edildi. Biyokimyasal analizler yapıldı.
Bulgular ve Sonuç: Sham grubuna kıyasla CLP grubunda TNF-a, OSI, IL-1B, TOS, MDA düzeyleri ve MPO aktivitesi yükselirken SOD ve TAS değerleri azaldı. Öte yandan,
Apokinin uygulaması sonucunda MPO aktivitesi, TNF-α, IL-1β, TOS, OSI ve MDA seviyeleri düşerken SOD ve TAS düzeyleri artmıştır. Sonuç olarak, Apokinin CLP kaynaklı
akciğer ve böbrek hasarına karşı etkili bir ajandır. 

Keywords

• Apokinin
• çekal ligasyon ve ponksiyon
• akciğer
• böbrek
• sıçan

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