Apigenin Deneysel Renal İskemi-Reperfüzyon Hasarına Karşı Etkili Bir Terapötik Ajan Olabilir mi?

Year 2024, Volume: 12 Issue: 2, 53 - 56, 31.07.2024

Ömer Topdağı , Mustafa Can Güler , Ersen Eraslan , Fazile Nur Ekinci Akdemir , Ayhan Tanyeli , Saime Özbek Şebin , Engin Şebin

https://doi.org/10.21765/pprjournal.1487201

Abstract

Amaç: Bu çalışma, iki doz apigenin (API) kullanımının renal iskemi-reperfüzyon hasarına (R I/R) karşı etkilerini ortaya koymayı amaçlamaktadır.
Gereç ve Yöntem: Bu amaçla çalışmamızda API'nin 5 ve 10 mg/kg dozları tercih edilmiş ve deneysel protokolün uygulanması için gruplar sham, R I/R, 5 mg/kg API ve 10 mg/kg API grupları olarak tasarlanmıştır. R I/R modelinde 1 saatlik iskemi ve 24 saatlik reperfüzyon periyotları tercih edilmiştir. Deney sonunda alınan örneklerde oksidatif ve inflamatuvar belirteçler biyokimyasal olarak ölçüldü.
Bulgular: Biyokimyasal sonuçlar oksidatif ve inflamatuar belirteçlerin R I/R grubunda anlamlı olarak arttığını, ancak antioksidan aktivitelerin anlamlı olarak azaldığını gösterdi. 5 ve 10 mg/kg API gruplarında, R I/R hasarı önemli ölçüde hafifledi ve bu belirteçler sham grubu değerlerine yaklaştı.
Sonuç: Sonuç olarak, çalışmanın sonuçları iki farklı API dozunun R I/R ile indüklenen böbrek hasarına karşı etkili olduğunu göstermiştir.

Keywords

Apigenin, böbrek, iskemi/reperfüzyon

Can Apigenin Be an Effective Therapeutic Agent Against Experimental Renal Ischemia-Reperfusion Injury?

Abstract

Aim: This study aims to reveal the effects of two doses of apigenin (API) against renal ischemia-reperfusion injury (R I/R).
Material and Methods: For this purpose, 5 and 10 mg/kg doses of API were preferred in our study, and the groups were designed as sham, R I/R, 5 mg/kg API, and 10 mg/kg API groups for the implementation of the experimental protocol. In the R I/R model, 1-hour ischemia and 24-hour reperfusion periods were preferred. Oxidative and inflammatory markers were measured biochemically in samples taken at the end of the experiment.
Results: Biochemical results showed that oxidative and inflammatory markers increased significantly in the R I/R group, but antioxidant activities decreased significantly. In the 5 and 10 mg/kg API groups, R I/R damage was alleviated considerably, with these markers approaching the sham group values.
Conclusion: As a result, the study's results determined that two different doses of API were effective against R I/R-induced kidney damage.

Keywords

Apigenin, kidney, ischemia/reperfusion.

References

• 1. Eltzschig HK, Eckle T. Ischemia and reperfusion--from mechanism to translation. Nat Med. 2011;17(11):1391-401.
• 2. Lee YM, Lee G, Oh TI, Kim BM, Shim DW, Lee KH, et al. Inhibition of glutamine utilization sensitizes lung cancer cells to apigenin-induced apoptosis resulting from metabolic and oxidative stress. Int J Oncol. 2016;48(1):399-408.
• 3. Malek M, Nematbakhsh M. Renal ischemia/reperfusion injury; from pathophysiology to treatment. J Renal Inj Prev. 2015;4(2):20-7.
• 4. Sharfuddin AA, Molitoris BA. Pathophysiology of ischemic acute kidney injury. Nat Rev Nephrol. 2011;7(4):189-200.
• 5. Shan YG, Chen DQ, Hu B, Xu GT, Li WL, Jin Y, et al. Allicin ameliorates renal ischemia/reperfusion injury via inhibition of oxidative stress and inflammation in rats. Biomed Pharmacother. 2021;142.
• 6. Topdagi O, Tanyeli A, Akdemir FNE, Eraslan E, Guler MC, Comakli S. Preventive effects of fraxin on ischemia/reperfusion-induced acute kidney injury in rats. Life Sci. 2020;242:117217.
• 7. Alsadat AM, Nikbakht F, Nia HH, Golab F, Khadem Y, Barati M, et al. GSK-3β as a target for apigenin-induced neuroprotection against Aβ 25-35 in a rat model of Alzheimer's disease. Neuropeptides. 2021;90.
• 8. Sung B, Chung HY, Kim ND. Role of Apigenin in Cancer Prevention via the Induction of Apoptosis and Autophagy. J Cancer Prev. 2016;21(4):216-26.
• 9. Berköz M, Yalın S, Özkan-Yılmaz F, Özlüer-Hunt A, Krośniak M, Francik R, et al. Protective effect of myricetin, apigenin, and hesperidin pretreatments on cyclophosphamide-induced immunosuppression. Immunopharmacol Immunotoxicol. 2021;43(3):353-69.
• 10. Berköz M, Ünal S, Karayakar F, Yunusoğlu O, Özkan-Yılmaz F, Özlüer-Hunt A, et al. Prophylactic effect of myricetin and apigenin against lipopolysaccharide-induced acute liver injury. Mol Biol Rep. 2021;48(9):6363-73.
• 11. Zare MFR, Rakhshan K, Aboutaleb N, Nikbakht F, Naderi N, Bakhshesh M, et al. Apigenin attenuates doxorubicin induced cardiotoxicity via reducing oxidative stress and apoptosis in male rats. Life Sciences. 2019;232.
• 12. Wang F, Zhou RJ, Zhao X, Ye H, Xie ML. Apigenin inhibits ethanol-induced oxidative stress and LPS-induced inflammatory cytokine production in cultured rat hepatocytes. J Appl Biomed. 2018;16(1):75-80.
• 13. Bradley PP, Priebat DA, Christensen RD, Rothstein G. Measurement of Cutaneous Inflammation - Estimation of Neutrophil Content with an Enzyme Marker. J Invest Dermatol. 1982;78(3):206-9.
• 14. Ohkawa H, Ohishi N, Yagi K. Assay for Lipid Peroxides in Animal-Tissues by Thiobarbituric Acid Reaction. Anal Biochem. 1979;95(2):351-8.
• 15. Sun Y, Oberley LW, Li Y. A Simple Method for Clinical Assay of Superoxide-Dismutase. Clin Chem. 1988;34(3):497-500.
• 16. Ak T, Gülçin I. Antioxidant and radical scavenging properties of curcumin. Chem-Biol Interact. 2008;174(1):27-37.
• 17. Gülçin I. Antioxidant activity of food constituents: an overview. Arch Toxicol. 2012;86(3):345-91.
• 18. Guler MC, Tanyeli A, Akdemir FNE, Eraslan E, Sebin SO, Erdogan DG, et al. An Overview of Ischemia-Reperfusion Injury: Review on Oxidative Stress and Inflammatory Response. Eurasian J Med. 2022;54:S62-S5.
• 19. Pomarede N, Chandramouli M. Enhancing the Skin's Natural Antioxidant Enzyme System by the Supplementation or Upregulation of Superoxide Dismutase, Catalase, and Glutathione Peroxidase. Nutritional Cosmetics: Beauty from Within. 2009:245-65.
• 20. Akdemir FNE, Yildirim S, Kandemir FM. The possible beneficial impacts of evodiamine on hepatotoxicity induced by cisplatin. Environ Sci Pollut R. 2022;29(59):89522-9.
• 21. Eraslan E, Tanyeli A, Ekinci Akdemir FN, Guler MC. Reno-Protective Effects of 6-Shogaol on Kidney Tissue in Cecal Ligation and Puncture-Induced Polymicrobial Sepsis Rat Model. Atatürk University Journal of Veterinary Sciences. 2020;15(3):244 - 50.
• 22. Guler MC, Tanyeli A, Eraslan E, Ekinci Akdemir FN. Role of 6-shogaol against ovarian torsion detorsion-induced reproductive organ damage. New Trends in Medicine Sciences. 2020;1(1):29-34.
• 23. Klebanoff SJ. Myeloperoxidase: friend and foe. J Leukocyte Biol. 2005;77(5):598-625.
• 24. Kvietys PR, Granger DN. Role of reactive oxygen and nitrogen species in the vascular responses to inflammation. Free Radical Bio Med. 2012;52(3):556-92.
• 25. Ko SH, Jun JH, Oh JE, Shin E, Kwak YL, Shim JK. Effect of high-dose vitamin C on renal ischemia-reperfusion injury. Biomed Pharmacother. 2024;173:116407.
• 26. Zhu JN, Shen H, Li GH, Chen L, Kang P, Guo YL, et al. Theaflavin pretreatment ameliorates renal ischemia/reperfusion injury by attenuating apoptosis and oxidative stress in vivo and in vitro. Biomed Pharmacother. 2024;171.
• 27. Wang H, Guo JS, Ding XF, Li Y, Xu H, Tian XY, et al. Fullerenol mitigates ischemia/reperfusion-induced kidney injury. Chem Eng J. 2024;485.
• 28. Cai SQ, Tang ZM, Xiong C, Wu FF, Zhao JR, Zhang Q, et al. The anti-inflammatory effects of apigenin and genistein on the rat intestinal epithelial (IEC-6) cells with TNF-alpha stimulation in response to heat treatment. Curr Res Food Sci. 2022;5:918-26.
• 29. Haleagrahara N, Chakravarthi S, Kulur AB, Yee TM. Plant flavone apigenin protects against cyclosporine-induced histological and biochemical changes in the kidney in rats. Biomedicine & Preventive Nutrition. 214;4(4):589-93.
• 30. Liu Y, Wang L, Du Y, Chen Z, Guo J, Weng X, et al. Effects of apigenin pretreatment against renal ischemia/reperfusion injury via activation of the JAK2/STAT3 pathway. Biomed Pharmacother. 2017;95:1799-808.
• 31. Wang X, Wang W, Wang JZ, Yang C, Liang CZ. Effect of apigenin on apoptosis induced by renal ischemia/reperfusion injury in vivo and in vitro. Ren Fail. 2018;40(1):498-505.
• 32. Liu Y, Liu X, Wang L, Du Y, Chen Z, Chen H, et al. Effects of apigenin on the expression levels of B-cell lymphoma-2, Fas and Fas ligand in renal ischemia-reperfusion injury in rats. Exp Ther Med. 2017;14(6):5345-54.