Anticancer Activity of Endemic Phlomis Extracts in HCT116 Human Colon Cancer Cells

Yıl 2023, , 227 - 233, 10.05.2023

Yener Kurman , Ozge Pasin

https://doi.org/10.33631/sabd.1208357

Öz

Aim: Previous studies have reported that Phlomis russeliana shows cytotoxic effects against several cancer cell lines; however, its anti cancer activity on HCT-116 cancer cells has not yet been investigated. Therefore, the present study is designed to explore anti-cancer properties of Phlomis russeliana against HCT-116 human colon cancer cell line and HUVEC normal cell line.
Material and Methods: HCT-116 cells and HUVECs treated with different concentrations of Phlomis russeliana (2, 4, 6, 8 and 10 mg/ml) and cell viability evaluated by the MTT assay. Anti-migratory and anti-colonigenic effects of Phlomis russeliana were assessed with wound healing and colony formation assays respectively. Quantitative determination of total antioxidant status (TAS), total oxidant status (TOS) and caspase-3 activation were performed with colorimetric Elisa kits.
Results: Phlomis russeliana significantly decreased cell viability of HCT-116 cells in a concentration dependent and showed weaker toxicity against normal HUVECs. Pholomis russeliana significantly inhibited migration and colony formation potential of HCT-116 cells. A significant increase in caspase-3 activation was observed after treatment with Phlomis russeliana. Phlomis russeliana did not significantly affect the TAS and TOS level in HCT-116 cells.
Conclusion: These results revealed that Phlomis russeliana showed anti-cancer activity in human colon cancer cells, through the suppression of colony formation, inhibition of migration and induction of caspase-3 activation. Phlomis russeliana, could be a promising source for the development of new anti-cancer agents against cancer.

Anahtar Kelimeler

Phlomis russeliana, HCT-116 cells, migration, caspase-3 activation, colony formation

Proje Numarası

Yok

HCT116 İnsan Kolon Kanseri Hücrelerinde Endemik Phlomis Ekstraktlarının Antikanser Aktivitesi

Öz

Amaç: Önceki çalışmalar, Phlomis russeliana'nın çeşitli kanser hücre hatlarına karşı sitotoksik etkiler gösterdiğini bildirmiştir; ancak, HCT-116 kanser hücreleri üzerindeki anti kanser aktivitesi henüz araştırılmamıştır. Bu nedenle, bu çalışma Phlomis russeliana'nın HCT-116 insan kolon kanseri hücre dizisi ve HUVEC normal hücre dizisine karşı anti-kanser özelliklerini araştırmak üzere tasarlanmıştır.
Gereç ve Yöntemler: HCT-116 ve HUVEC hücreleri Phlomis russeliana’nın farklı konsantrasyonları (2, 4, 6, 8 ve 10 mg/ml) ile muamele edildi ve hücre canlılığı MTT yöntemi ile değerlendirildi. Phlomis russeliana'nın anti-migrasyon ve anti-kolonijenik etkileri, sırasıyla yara iyileşmesi ve koloni oluşumu analizleri ile değerlendirildi. Kolorimetrik Elisa kitleri ile toplam antioksidan durumunun (TAS), toplam oksidan durumunun (TOS) ve kaspaz-3 aktivasyonunun kantitatif tespiti yapıldı.
Bulgular: Phlomis russeliana, konsantrasyona bağlı olarak HCT-116 hücrelerinin canlılığını önemli ölçüde azalttı ve normal HUVEC'lere karşı daha zayıf toksisite gösterdi. Phlomis russeliana, HCT-116 hücrelerinin göçünü ve koloni oluşturma potansiyelini önemli ölçüde engelledi. Phlomis russeliana ile tedaviden sonra kaspaz-3 aktivasyonunda önemli bir artış gözlendi. Phlomis russeliana, HCT-116 hücrelerinde TAS ve TOS seviyesini önemli ölçüde etkilemedi.
Sonuç: Bu sonuçlar; Phlomis russeliana'nın koloni oluşumunun baskılanması, migrasyonun engellenmesi ve kaspaz-3 aktivasyonunun indüklenmesi yoluyla insan kolon kanseri hücrelerinde anti-kanser aktivitesi gösterdiğini ortaya koydu. Phlomis russeliana, kansere karşı yeni anti-kanser ajanlarının geliştirilmesi için umut verici bir kaynak olabilir.

Anahtar Kelimeler

Phlomis russeliana, HCT-116 hücreleri, migrasyon, kaspaz-3 aktivasyonu, koloni oluşumu

Destekleyen Kurum

Yok

Proje Numarası

Yok

Kaynakça

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