İdrar Örneklerinden İzole Edilen Streptococcus agalactiae suşlarının Çeşitli Antibiyotiklere Direnç Oranları

Yıl 2023, , 373 - 377, 21.09.2023

İsmail Selçuk Aygar

https://doi.org/10.33631/sabd.1311932

Öz

Amaç: Streptococcus agalactiae olarak da bilinen Grup B Streptokoklar (GBS) insan normal gastrointestinal ve ürogenital florasında bulunan Gram pozitif, kapsüllü bakterilerdir. Yenidoğanlarda, yaşlılarda, bağışıklığı baskılanmış kişilerde çok çeşitli bulaşıcı hastalıklar kişilerde, gebelerde ve erişkinlerde ürogenital sistem enfeksiyonlarına neden olabilirler. Bu çalışmada Kadın Hastalıkları ve Doğum Kliniği'nden laboratuvarımıza gelen idrar örneklerinden izole edilen GBS suşlarının antibiyotik duyarlılıklarını inceleyerek intrapartum antibiyotik profilaksisinde kullanılabilecek ajanlar hakkında klinisyenleri bilgilendirmek amaçlandı.
Gereç ve Yöntemler: Ocak 2022- Aralık 2022 yılları arasında Kadın Hastalıkları ve Doğum Kliniği'nden Tıbbi Mikrobiyoloji laboratuvarımıza gönderilen toplam 222 klinik idrar örneğinden izole edilen S. agalactiae izolatları dahil edildi ve bu izolatlara ait veriler retrospektif olarak değerlendirildi. Laboratuvarımıza gelen klinik örnekler Koyun kanlı agar besiyerine ekildi ve 37°C'de 18-24 saat inkübe edildi. Kantitatif olarak ekim yapılan idrar kültüründe ≥104 CFU/ml üremesi olan bakteriler üropatojen olarak kabul edilirken ürogenital cilt flora elemanı veya üç ve daha fazla farklı tipte bakteri üreyen kültürler kontaminasyon olarak değerlendirildi.
Bulgular: Seçilen hasta grubu nedeniyle hastaların %100’ü kadındı. Hastaların yaşları 12 ile 72 arasında değişirken yaş ortalaması ise 30,16’ydı. GBS izolatlarının tümü penisilin, linezolid, trimetoprim sulfametoksazol, tigesiklin, teikoplanin ve vankomisine duyarlıydı.
Sonuç: Çalışmamızda penisilin direnci saptanmamıştır. Ancak penisilinin kullanılamadığı durumlarda diğer seçenekler olarak öne çıkan eritromisin, klindamisin ve levofloksasine dirençli suşlar tespit edilmiştir.

Anahtar Kelimeler

Grup B streptokok, penisilin, antimikrobiyal duyarlılık

Resistance Rates of Streptococcus agalactiae Strains Isolated from Urine Samples to Various Antibiotics

Öz

Aim: Group B Streptococcus (GBS), also known as Streptococcus agalactiae, are Gram-positive, encapsulated bacteria found in the normal human gastrointestinal and urogenital flora. A wide variety of infectious diseases can cause urogenital system infections in newborns, elderly people, immunocompromised people, pregnant women, and adults. In this study, we aimed to inform clinicians about the agents that can be used in intrapartum antibiotic prophylaxis by examining the antibiotic susceptibility of GBS strains isolated from urine samples from the Gynecology and Obstetrics Clinic in our laboratory.
Material and Methods: Isolates of the S. agalactiae isolated from a total of 222 urine samples from the Gynecology and Obstetrics Clinic that came to our Medical Microbiology laboratory between January 2022 and December 2022 were included and the data of these isolates were evaluated retrospectively. The clinical samples that came to our laboratory were inoculated on Sheep blood agar medium and incubated at 37°C for 18-24 hours. Quantitatively seeded urine culture ≥104 CFU/ml grown bacteria was considered a uropathogenic. Urogenital skin flora element or three and more different types of bacteria growing cultures as contamination evaluated.
Results: Due to the group selected as the sample, 100% of the patients were female. The age range of the patients ranged from 12 to 72, and the mean age was calculated as 30.16. All GBS isolates were susceptible to penicillin, linezolid, trimethoprim-sulfamethoxazole, tigecycline, teicoplanin, and vancomycin.
Conclusion: Penicillin resistance was not found in our study. However, resistant strains were detected in erythromycin, clindamycin, and levofloxacin, which stand out as other options in cases where penicillin cannot be used.

Anahtar Kelimeler

Group B streptococcus, penicillin, antimicrobial susceptibility

Kaynakça

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