Hareketsizlik Stresinin Sıçan Testis Dokusu Üzerindeki Histopatolojik Etkileri ve Lavanta Esansiyel Yağının Koruyucu Rolü

Abstract

Amaç: Stres çeşitli fizyolojik ve psikolojik bozukluklara yol açabilir ve ayrıca erkek üreme sağlığı üzerinde önemli bir etkiye sahiptir. Stresin kadın doğurganlığı üzerindeki etkileri kapsamlı bir şekilde incelenmiş olsa da, erkek doğurganlığı üzerindeki etkisi nispeten az araştırılmıştır. Bu çalışma, lavanta esansiyel yağının (LEO) sıçanlarda immobilizasyon stresi ile indüklenen testis hasarına karşı potansiyel koruyucu etkisini incelemeyi amaçlamıştır. Gereç ve Yöntemler: Yirmi dört erkek Wistar Albino sıçanı rastgele dört gruba ayrıldı: Kontrol (C), Stres (S), LEO (L) and Stres + LEO (S+L). Stres gruplarına 14 gün boyunca immobilizasyon stresi uygulandı ve LEO ilgili gruplara inhalasyon yoluyla verildi. Deneyin sonunda elde edilen testis dokuları histopatolojik değerlendirme için hematoksilin ve eozin ve Masson trikromu ile boyandı. Tüm grupların hematoksilin-eozin boyalı testis kesitleri yarı kantitatif olarak değerlendirildi. Bulgular: Bu değerlendirme, S grubundaki testis dokularında önemli dejeneratif değişiklikler, düzensiz seminifer tübüller ve interstisyel ödem ortaya koydu. Buna karşılık, S + L grubundaki stres kaynaklı hasarlı doku, morfolojide önemli bir iyileşme gösterdi. Bu bulgular, LEO'nun antioksidan ve anti-inflamatuar özellikleri nedeniyle immobilizasyon stres kaynaklı testis hasarını hafifletebileceğini düşündürmektedir. Sonuç: Bu çalışma, lavanta esansiyel yağının (LEO), immobilizasyon stresi ile indüklenen testiküler hasarı hafifletmede potansiyel koruyucu etkileri olduğunu göstermektedir. LEO uygulaması, stresin neden olduğu histopatolojik bozuklukları azaltarak testis dokusunun yapısal bütünlüğünü kısmen korumuştur. Bu sonuçlar, LEO’nun stres kaynaklı erkek üreme hasarına karşı alternatif bir destekleyici tedavi olarak değerlendirilebileceğini düşündürmektedir.

Keywords

• Hareketsizleştirme stresi
• lavanta yağı
• testis dokusu

The Histopathological Effects of Immobilization Stress on Rat Testis Tissue, and The Protective Role of Lavender Essential Oil

Abstract

Aim: Stress can lead to various physiological and psychological disorders and also has a significant effect on male reproductive health. Although the effects of stress on female fertility have been extensively studied, its impact on male fertility is relatively under-researched. This study set out to examine the potential protective effect of lavender essential oil (LEO) against testicular damage induced by immobilization stress in rats. Material and Methods: Twenty-four male Wistar Albino rats were randomly divided into four groups: Control (C), Stress (S), LEO (L) and Stress + LEO (S+L). Immobilization stress was applied to the S groups for 14 days, and LEO was administered to the relevant groups by inhalation. The testicular tissues obtained at the end of the experiment were stained with hematoxylin & eosin and Masson’s trichrome for histopathological evaluation. Hematoxylin-eosin-stained testicular sections of all groups were evaluated semi-quantitatively. Results: That evaluation revealed significant degenerative changes, irregular seminiferous tubules, and interstitial edema in the testicular tissues from the S group. In contrast, the stress-induced damaged tissue in the S + L group exhibited a significant improvement in morphology. Conclusion: This study demonstrates the potential protective effects of LEO against testicular damage induced by immobilization stress. The administration of LEO partially preserved the structural integrity of the testicular tissue by mitigating histopathological alterations caused by stress. These findings suggest that LEO may be considered as a supportive therapeutic agent against stress-related male reproductive damage.

Keywords

• Immobilization stress
• testis tissue
• lavender essential oil

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