METHYLATION AND EXPRESSION VALIDATION RESULTS OF GPRC5C GENE OBTAINED FROM EPIGENOMICS AND TRANSCRIPTOMICS DATA IN ORAL CAVITY TUMORS
Öz
Objective: Demokan et.al idetified G/Protein/Coupled/Receptor/Class-C/Group-5/Member-C (GPRC5C) as a candidate gene in OSCC via expression and
methylation array results (logFC:0.59663; DeltaBeta: -0.130984561) from their previous unpublished data. In our study, the methylation and expression
status of GPRC5C gene was investigated in a larger group of OSCC patients and healthy cohort for further validation. Its potential candidate biomarker was
evaluated for early diagnosis of OSCC by non-invasive methods.
Materials and Methods: DNA and RNA were isolated from tissues/body fluid samples of 30 patients OSCC and 15 healthy individuals. The methylation and
expression levels were analyzed by using QMSP and QRT-PCR methods, respectively. The data were compared with clinical parameters and evaluated by
statistical analysis.
Results: Decreased expression levels were observed in 43% and increased expression levels were observed in 33% in the tumor tissues compared to
corresponding-normal tissues of OSCC patients. Methylation-based expression loss was observed in 4 patients having the ratio between 3.4 and 12.2%.
We also observed a decrease in expression in OSCC patients with observed methylation. There was statistical significance between differentiation degree
and expression in OSCC.
Conclusion: It was concluded that GPRC5C may play a role as a potential methylation biomarker candidate of specific subgroups of the oral cavity. Other
mechanisms need to be explored to understand the differences in expression levels.
Anahtar Kelimeler
Kaynakça
- Grozescu T, Popa F. Prostate cancer between prognosis and adequate/proper therapy. J Med Life. 2017;10(1):5-12
METHYLATION AND EXPRESSION VALIDATION RESULTS OF GPRC5C GENE OBTAINED FROM EPIGENOMICS AND TRANSCRIPTOMICS DATA IN ORAL CAVITY TUMORS
Öz
Objective: Demokan et.al idetified G/Protein/Coupled/Receptor/Class-C/Group-5/Member-C (GPRC5C) as a candidate gene in OSCC via expression and
methylation array results (logFC:0.59663; DeltaBeta: -0.130984561) from their previous unpublished data. In our study, the methylation and expression
status of GPRC5C gene was investigated in a larger group of OSCC patients and healthy cohort for further validation. Its potential candidate biomarker was
evaluated for early diagnosis of OSCC by non-invasive methods.
Materials and Methods: DNA and RNA were isolated from tissues/body fluid samples of 30 patients OSCC and 15 healthy individuals. The methylation and
expression levels were analyzed by using QMSP and QRT-PCR methods, respectively. The data were compared with clinical parameters and evaluated by
statistical analysis.
Results: Decreased expression levels were observed in 43% and increased expression levels were observed in 33% in the tumor tissues compared to
corresponding-normal tissues of OSCC patients. Methylation-based expression loss was observed in 4 patients having the ratio between 3.4 and 12.2%.
We also observed a decrease in expression in OSCC patients with observed methylation. There was statistical significance between differentiation degree
and expression in OSCC.
Conclusion: It was concluded that GPRC5C may play a role as a potential methylation biomarker candidate of specific subgroups of the oral cavity. Other
mechanisms need to be explored to understand the differences in expression levels.
Anahtar Kelimeler
Kaynakça
- Grozescu T, Popa F. Prostate cancer between prognosis and adequate/proper therapy. J Med Life. 2017;10(1):5-12
Ayrıntılar
| Birincil Dil | İngilizce |
|---|---|
| Konular | Klinik Tıp Bilimleri |
| Bölüm | Toplantı Özeti |
| Yazarlar | |
| Yayımlanma Tarihi | 9 Ağustos 2022 |
| Gönderilme Tarihi | 30 Haziran 2022 |
| Yayımlandığı Sayı | Yıl 2022 |