The Effects of Green Tea Leaf Extract on Cytogenetical and Physiological Parameters of Allium cepa L. exposed to Salinity

Yıl 2020, , 338 - 346, 01.04.2020

Dilek Çavuşoğlu

https://doi.org/10.16984/saufenbilder.541835

Cited By: 3

Öz

The aim of this study investigated the effects of 50 mg L-1 green tea leaf extract in decreasing harmful effects of 0.175 M salinity stress on the mitotic activity, chromosomal aberrations, seedling growth (fresh weight, radicle length and radicle number), micronucleus frequency which is the simplest indicator, the most effective of cytological damage and bulb germination of A. cepa L. In only green tea leaf extract medium, the radicle length and radicle number of bulbs were partially reduced compared to the control bulbs germinated in the distilled water medium. While their germination percentage and fresh weight statistically indicated the same values. Besides, the mitotic index and chromosomal abnormalities in the root tip meristematic cells of Allium cepa bulbs germinated in alone green tea leaf extract medium increased compared to germinated control bulbs in the distilled water medium, whereas the micronucleus frequency showed statistically the same value compared to the control. In other words, it can be said that salt stress significantly inhibited the seedling growth and bulb germination of Allium cepa. What’s more, it significantly reduced the mitotic index in the root tip meristems of the bulbs and increased the number of chromosomal abnormalities and micronuclues frequency. On the other hand, inhibitory effects of salt on the mitotic activity, seedling growth, bulb germination, chromosomal abnormalities and micronuclues frequency significantly decreased with the application of green tea leaf extract. The germination percentage, radicle lenght, radicle number, fresh weight, mitotic index, micronuclues frequency and chromosomal aberrations of the seedlings grown in 0.175 M salinity were 23 %, 10.3 mm, 12.7, 7 g, 1.2 %, 13 % and 17 % respectively, while these values became 75 %, 13.4 mm, 17.2, 13.8 g, 6.3 %, 9 % and 9.3 % in the seedlings treated with 50 mg L-1 green tea leaf extract.  

Anahtar Kelimeler

green tea leaf extract, chromosomal aberrations, mitotic index, salt stress, seed germination

Kaynakça

• Referans 1 T.J. Flowers and A.R. Yeo, “Ion relations of plant under drought and salinity”, Australian Journal of Plant Physiology, vol. 13, pp. 75–91, 1986.
• Referans 2 T.J. Flowers and A.R. Yeo, “Breeding for salinity resistance in crop plants: where next?”, Australian Journal of Plant Physiology, vol. 22, pp. 875–884, 1995.
• Referans 3 H. Basaga, C. Tekkaya, and F. Acikel, “Antioxidative and free radical scavenging properties of rosemary extract”, Lebensmittel-Wissenschaft Technologie, vol. 30, pp. 105–8, 1997.
• Referans 4 K. Dvorakova, R.T. Dorr, S. Valcic, B. Timmermann, and D.S. Alberts, “Pharmacokinetics of the green tea derivative, EGCG, by the topical route of administration in mouse and human skin”, Cancer Chemotheraphy and Pharmacology, vol. 43, pp. 331-335, 1999.
• Referans 5 V. Crespy and G. Williamson, “A review of the health effects of green tea catechins in vivo animal models”, The Journal of Nutrition, vol. 134, pp. 3431–3440, 2004.
• Referans 6 D.G. Nagle, D. Ferreira, and Y.D. Zhou, “Molecules of interest epigallocatechin-3-gallate (EGCG): chemical and biomedical perspectives”, Phytochemistry, vol. 67, pp. 1849–1855, 2006.
• Referans 7 S.Y. Asadi, P. Parsaei, M. Karimi, S. Ezzati, A. Zamiri, F. Mohammadizadeh, and M. Rafieian-Kopaei, “Effect of green tea (Camellia sinensis) extract on healing process of surgical wounds in rat”, International Journal of Surgery, vol. 11, no. 4, pp. 332-7, 2013.
• Referans 8 G. Fiskesjo, “The Allium test as a standard in environmental monitoring”, Hereditas, vol. 102, no. 1, pp. 99–112, 1985.
• Referans 9 W.F. Grant, “Chromosome aberration assays in Allium: a report of the united states environmental protection agency’s gene-tox program”, Mutation Research/Reviews in Genetic Toxicology, vol. 99, pp. 273–291, 1982.
• Referans 10 D.B. Duncan, “Multiple range and multiple F tests”, Biometrics, vol. 11, pp. 1-42, 1955.
• Referans 11 K. Çavuşoğlu, S. Cadıl, and D. Çavuşoğlu, “Role of potassium nitrate (KNO3) in alleviation of detrimental effects of salt stress on some physiological and cytogenetical parameters in Allium cepa L.”, Cytologia, vol. 82, no. 3, pp. 279-286, 2017.
• Referans 12 D. Çavuşoğlu, K. Çavuşoğlu, and S. Tabur, “The effects of black cumin (Nigella sativa L.) seed extract on the seed germination, seedling growth, mitotic activity and chromosomal abberations of Allium cepa L. under saline condition”, ARPN Journal of Agricultural and Biological Science, vol. 13, no. 5, pp. 50-57, 2018.
• Referans 13 D. Çavuşoğlu, S. Tabur, and K. Çavuşoğlu, “The effects of Aloe vera L. leaf extract on some physiological and cytogenetical parameters in Allium cepa L. seeds germinated under salt stress”, Cytologia, vol. 81, no. 1, pp. 103-110, 2016a.
• Referans 14 D. Çavuşoğlu, S. Tabur, and K. Çavuşoğlu, “Role of Ginkgo biloba L. leaf extract on some physiological and cytogenetical parameters in Allium cepa L. seeds exposed to salt stress”, Cytologia, vol. 81, no. 2, pp. 207-213, 2016b.
• Referans 15 A.İ. İlbaş, U. Gönen, S. Yılmaz, and M.Y. Dadandı, “Cytotoxicity of Aloe vera gel extracts on Allium cepa root tip cells”, Turkish Journal of Botany, vol. 36, pp. 263-268, 2012.
• Referans 16 G.O. Alege and B.O. Ojomah, “ Cytotoxic effects of Aloe vera leaf extract on Allium sativum root tips”, European Journal of Experimental Biology, vol. 4, pp. 9-14, 2014.
• Referans 17 Y. Eren and A. Özata, “Determination of mutagenic and cytotoxic effects of Limonium globuliferum aqueous ectracts by Allium, Ames, and MTT tests”, Revista Brasileira de Farmacognosia, vol. 24, pp. 51-59, 2014.
• Referans 18 B, Kalpana, S. Lakshmi, and S. Karthikeyan, “Effect of fresh leaf extract of tobacco on the somatic chromosome of Allium cepa Linn”, International Journal of Current Research, vol. 3, pp. 109-112, 2014.
• Referans 19 MC. Karaismailoğlu, “Investigation of the cytotoxic and genotoxic effects of Artemisia annua methanol extract with the Allium test”, Ekoloji, vol. 23, pp. 64-74, 2014.
• Referans 20 S.Siddiqui, “Genotoxic effect of four medicinal plant extracts on Pisum sativum cv. Arikil”, Bangladesh Journal of Botany, vol. 43, pp. 107-111, 2014.
• Referans 21 N.K. Gupta, S.K. Meena, S. Gupta, and S.K. Khandelwal, “Gas exchange, membrane permeability, and ion uptake in two species of Indian jujube differing in salt tolerance”, Photosynthetica, vol. 40, pp. 535-539, 2002.
• Referans 22 R. Munns, “Comparative physiology of salt and water stress”, Plant Cell & Environment, vol. 25, pp. 239-250, 2002.
• Referans 23 W.Y. Kao, T.T. Tsai, and C.N. Shih, “Photosynthetic gas exchange and chlorophyll a fluorescence of three wild soybean species in response to NaCl treatments”, Photosynthetica, vol. 41, pp. 415-419, 2003.
• Referans 24 O.H. Sayed, “Chlorophyll fluorescence as a tool in cereal crop research”, Photosynthetica, vol. 41, pp. 321-330, 2003.
• Referans 25 R.M. Ali, “Role of putrescine in salt tolerance of Atropa belladonna plant”, Plant Science, vol. 152, pp. 173–179, 2000.
• Referans 26 K.F. Mccue and A.D. Hanson, “Drought and salt tolerance: towards understanding and application”, Trends Biotechnology, vol. 8, pp. 358-362, 1990.
• Referans 27 L. Prakash, M. Dutt, and G. Prathapasenan, “NaCl alters contents of nucleic acids, protein, polyamines and seedling growth of rice (Oryza sativa L.)”, Australian Journal of Plant Physiology, vol. 15, pp. 769-776, 1988.
• Referans 28 A.A. Mariod, R.M. İbrahim, M. İsmail, and N. İsmail, “Antioxidant activity and phenolic content of phenolic rich fractions obtained from black cumin (Nigella sativa) seedcake”, Food Chemistry, vol. 116, pp. 306-312, 2009.
• Referans 29 H. Nefic, J. Musanovic, A. Metovic, and K. Kurteshi, “Chromosomal and nuclear alterations in root tip cells of Allium cepa L. induced by alprazolam”, Medical Archives, vol. 67, pp. 388–392, 2013.
• Referans 30 S. Radic, M. Prolic, M. Pavlica, and B. Pevalek-Kozlina, “Cytogenetic effects of osmotic stress on the root meristem cells of Centaurea ragusina L.”, Environmental and Experimental Botany, vol. 54, pp. 213-218, 2005.
• Referans 31 M. Kara, M.A. Tanda, and A. Ateş, “Cytogenetic effects of the insecticide cypermethin on the root meristems of Allium cepa L.” Turkish Journal of Biology, vol. 18, pp. 323–331, 1994.
• Referans 32 F.I. Akaneme and I.V. Iyioke, “Mutagenic potentials of the sterilizing fluid-purital on root tip mitosis of Allium cepa”, Bio-Research, vol. 6, pp. 293–297, 2008.
• Referans 33 P.K. Renjana, S. Anjana, and J.E. Thoppil, “Evaluation of genotoxic effects of baking powder and monosodium glutamate (MSG) using Allium cepa assay”, International Journal of Pharmacy and Pharmaceutical Sciences, vol. 5, 132-139, 2013.
• Referans 34 D.M. Leme and M.A. Marin-Morales, “Allium cepa test in environmental monitoring: a review on its application”, Mutation Research, vol. 682, pp. 71–81, 2009.
• Referans 35 N. Khanna and S. Sharma, “Allium cepa root chromosomal aberration assay”, Indian Journal of Pharmaceutical and Biological Research, vol. 1, pp. 105-119, 2013.
• Referans 36 S. Türkoğlu, “Genotoxicity of five food preservatives tested on root tips of Allium cepa L.” Mutation Research, vol. 626, pp. 4-14, 2007.
• Referans 37 S.M. Amer and E.M. Alı, “Cytological effects of pesticides. V. effects of some herbicides on Vicia faba”, Cytologia, vol. 39, pp. 633-643, 1974.